Journal Article
- iTRAQ-facilitated proteomic analysis of Bacillus cereus via degradation of malachite green
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Bobo Wang , Jing Lu , Junfang Zheng , Zhisheng Yu
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J. Microbiol. 2021;59(2):142-150. Published online February 1, 2021
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DOI: https://doi.org/10.1007/s12275-021-0441-0
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Abstract
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The wide use of malachite green (MG) as a dye has caused
substantial concern owing to its toxicity. Bacillus cereus can
against the toxic effect of MG and efficiently decolourise it.
However, detailed information regarding its underlying adaptation
and degradation mechanisms based on proteomic
data is scarce. In this study, the isobaric tags for relative and
absolute quantitation (iTRAQ)-facilitated quantitative method
was applied to analyse the molecular mechanisms by
which B. cereus degrades MG. Based on this analysis, 209
upregulated proteins and 198 downregulated proteins were
identified with a false discovery rate of 1% or less during MG
biodegradation. Gene ontology and KEGG analysis determined
that the differentially expressed proteins were enriched
in metabolic processes, catalytic activity, antioxidant activity,
and responses to stimuli. Furthermore, real-time qPCR was
utilised to further confirm the regulated proteins involved
in benzoate degradation. The proteins BCE_4076 (Acetyl-CoA
acetyltransferase), BCE_5143 (Acetyl-CoA acetyltransferase),
BCE_5144 (3-hydroxyacyl-CoA dehydrogenase), BCE_4651
(Enoyl-CoA hydratase), and BCE_5474 (3-hydroxyacyl-CoA
dehydrogenase) involved in the benzoate degradation pathway
may play an important role in the biodegradation of MG
by B. cereus. The results of this study not only provide a comprehensive
view of proteomic changes in B. cereus upon MG
loading but also shed light on the mechanism underlying
MG biodegradation by B. cereus.
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- Engineering globins for efficient biodegradation of malachite green: two case studies of myoglobin and neuroglobin
Jiao Liu, Jia-Kun Xu, Hong Yuan, Xiao-Juan Wang, Shu-Qin Gao, Ge-Bo Wen, Xiang-Shi Tan, Ying-Wu Lin
RSC Advances.2022; 12(29): 18654. CrossRef
Reviews
- REVIEW] The contribution of Aspergillus fumigatus stress responses to virulence and antifungal resistance
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Neil A. Brown , Gustavo H. Goldman
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J. Microbiol. 2016;54(3):243-253. Published online February 27, 2016
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DOI: https://doi.org/10.1007/s12275-016-5510-4
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52
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Abstract
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Invasive aspergillosis has emerged as one of the most common
life-threatening fungal disease of humans. The emergence
of antifungal resistant pathogens represents a current
and increasing threat to society. In turn, new strategies to
combat fungal infection are urgently required. Fungal adaptations
to stresses experienced within the human host are a
prerequisite for the survival and virulence strategies of the
pathogen. Here, we review the latest information on the
signalling pathways in Aspergillus fumigatus that contribute
to stress adaptations and virulence, while highlighting their
potential as targets for the development of novel combinational
antifungal therapies.
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Citations
Citations to this article as recorded by

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- MINIREVIEW] Regulation of Escherichia coli RNase III activity
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Boram Lim , Minji Sim , Howoon Lee , Seogang Hyun , Younghoon Lee , Yoonsoo Hahn , Eunkyoung Shin , Kangseok Lee
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J. Microbiol. 2015;53(8):487-494. Published online July 31, 2015
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DOI: https://doi.org/10.1007/s12275-015-5323-x
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Abstract
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Bacterial cells respond to changes in the environment by
adjusting their physiological reactions. In cascades of cellular
responses to stresses of various origins, rapid modulation
of RNA function is known to be an effective biochemical
adaptation. Among many factors affecting RNA function,
RNase III, a member of the phylogenetically highly conserved
endoribonuclease III family, plays a key role in posttranscriptional
regulatory pathways in Escherichia coli. In
this review, we provide an overview of the factors affecting
RNase III activity in E. coli.
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Citations
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- Identification of [sigma]^B-Dependent Promoters Using Consensus-Directed Search of Streptomyces coelicolor Genome
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Eun-Jin Lee , You-Hee Cho , Hyo-Sub Kim , Jung-Hye Roe
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J. Microbiol. 2004;42(2):147-151.
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DOI: https://doi.org/2030 [pii]
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Abstract
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[sigma]^B plays an important role in both osmoprotection and proper differentiation in Streptomyces coelicolor A3(2). We searched for candidate members of the [sigma]^B regulon from the genome database, using the consensus promoter sequence (GNNTN_14-16GGGTAC/T). The list consists of 115 genes, and includes all the known [sigma]^B target genes and many other genes whose functions are related to stress protection and differentiation.