Journal of Microbiology

Journal Article

Genetic Characteristics and Phylogeographic Dynamics of Echovirus: Yan Wang , Pir Tariq Shah , Yue Liu , Amina Nawal Bahoussi , Li Xing; J. Microbiol. 2023;61(9):865-877. Published online September 15, 2023; DOI: https://doi.org/10.1007/s12275-023-00078-w

42 View
0 Download
1 Web of Science

Abstract: Echoviruses belong to the genus Enterovirus in the Picornaviridae family, forming a large group of Enterovirus B (EVB) within the Enteroviruses. Previously, Echoviruses were classified based on the coding sequence of VP1. In this study, we performed a reliable phylogenetic classification of 277 sequences isolated from 1992 to 2019 based on the full-length genomes of Echovirus. In this report, phylogenetic, phylogeographic, recombination, and amino acid variability landscape analyses were performed to reveal the evolutional characteristics of Echovirus worldwide. Echoviruses were clustered into nine major clades, e.g., G1–G9. Phylogeographic analysis showed that branches G2–G9 were linked to common strains, while the branch G1 was only linked to G5. In contrast, strains E12, E14, and E16 clustered separately from their G3 and G7 clades respectively, and became a separate branch. In addition, we identified a total of 93 recombination events, where most of the events occurred within the VP1-VP4 coding regions. Analysis of amino acid variation showed high variability in the a positions of VP2, VP1, and VP3. This study updates the phylogenetic and phylogeographic information of Echovirus and indicates that extensive recombination and significant amino acid variation in the capsid proteins drove the emergence of new strains.

Observational Study

Early gut microbiota in very low and extremely low birth weight preterm infants with feeding intolerance: a prospective case-control study: Ling Liu , Dang Ao , Xiangsheng Cai , Peiyi Huang , Nali Cai , Shaozhu Lin , Benqing Wu; J. Microbiol. 2022;60(10):1021-1031. Published online August 19, 2022; DOI: https://doi.org/10.1007/s12275-022-2180-2

57 View
0 Download
12 Web of Science
10 Crossref

The potential role of the gut microbiota in the pathogenesis of feeding intolerance (FI) remains unclear. Understanding the role of the gut microbiota could provide a new avenue for microbiota-targeted therapeutics. This study aimed to explore the associations between aberrant gut microbiota and FI in very low or extremely low birth weight (VLBW/ELBW) preterm infants. In this observational case-control study, VLBW/ ELBW infants were divided into two groups: FI group and feeding tolerance (FT) group. 16S rRNA gene sequencing was performed to analyze the gut microbial diversity and composition of the infants. The differences in the gut microbiota of the two groups were compared. In total, 165 stool samples were obtained from 44 infants, among which, 31 developed FI and 13 served as controls. Alpha diversity was the highest in the meconium samples of the two groups. LEfSe analysis revealed that the abundances of Peptostreptococcaceae, Clostridiales and Clostridia in the FT group were significantly higher than in the FI group. At the phylum level, the FI group was dominated by Proteobacteria, and the FT group was dominated by Firmicutes. The meconium samples of the FI group had higher proportions of γ-proteobacteria and Escherichia-Shigella and a lower proportion of Bacteroides compared with the FT group. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that aberrant gut bacteria in the FI group were strongly associated with dysregulation of C5-Brancheddibasic- acid-metabolism, protein kinases, and sporulation. These findings reveal candidate microbial markers to prevent FI. Increased relative abundances of γ-proteobacteria and Escherichia-Shigella and decreased abundance of Bacteroides in meconium were associated with an increased risk of FI, while Peptostreptococcaceae, Clostridiales and Clostridia reduced the risk of FI in VLBW/ELBW infants.

Citations

Citations to this article as recorded by

Reduced Gut Bacterial Diversity in Early Life Predicts Feeding Intolerance in Preterm Neonates
Maria Di Chiara, Alessandro Lazzaro, Daniela Scribano, Maria Trancassini, Valeria Pietropaolo, Michele Sonnessa, Chiara De Luca, Rita Prota, Elisa Onestà, Gianluigi Laccetta, Gianluca Terrin
Tropical Medicine and Infectious Disease.2024; 9(8): 174. CrossRef
Calorie restriction during gestation impacts maternal and offspring fecal microbiome in mice
Stephanie P. Gilley, Meghan L. Ruebel, Sree V. Chintapalli, Clyde J. Wright, Paul J. Rozance, Kartik Shankar
Frontiers in Endocrinology.2024;[Epub] CrossRef
Dynamics alteration of the gut microbiota and faecal metabolomes in very low or extremely low birth weight infants: a Chinese single-center, prospective cohort study
Ling Liu, Chaohong Chen, YeShan Li, Dang Ao, Jiayuan Wu, Nali Cai, Wen Li, Min Xiang
Frontiers in Microbiology.2024;[Epub] CrossRef
Metabolic and fecal microbial changes in adult fetal growth restricted mice
Stephanie P. Gilley, Miguel A. Zarate, Lijun Zheng, Purevsuren Jambal, Deaunabah N. Yazza, Sree V. Chintapalli, Paul S. MacLean, Clyde J. Wright, Paul J. Rozance, Kartik Shankar
Pediatric Research.2024; 95(3): 647. CrossRef
A digital twin of the infant microbiome to predict neurodevelopmental deficits
Nicholas Sizemore, Kaitlyn Oliphant, Ruolin Zheng, Camilia R. Martin, Erika C. Claud, Ishanu Chattopadhyay
Science Advances.2024;[Epub] CrossRef
Investigating prenatal and perinatal factors on meconium microbiota: a systematic review and cohort study
Jenni Turunen, Mysore V. Tejesvi, Niko Paalanne, Tytti Pokka, Sajeen Bahadur Amatya, Surbhi Mishra, Anna Kaisanlahti, Justus Reunanen, Terhi Tapiainen
Pediatric Research.2024; 95(1): 135. CrossRef
Novel scoring system for early diagnosis of necrotizing enterocolitis: integrating clinical and laboratory data with urinary caveolin-1 levels
Brigitta I.R.V. Corebima, Rinawati Rohsiswatmo, Dewi Santosaningsih, Wisnu Barlianto, Kusworini Handono
Archives of Medical Science.2023; 20(2): 444. CrossRef
Dynamics and Crosstalk between Gut Microbiota, Metabolome, and Fecal Calprotectin in Very Preterm Infants: Insights into Feeding Intolerance
Luyang Hong, Yihuang Huang, Junyan Han, Shujuan Li, Lan Zhang, Siyuan Jiang, Qi Zhou, Xincheng Cao, Weiyin Yu, Yi Yang, Shangyu Hong, Yufeng Zhou, Weili Yan, Yun Cao
Nutrients.2023; 15(22): 4849. CrossRef
Characteristics of Gut Microbiota in Small for Gestational Age Infants with Very Low Birth Weight
Hung-Yang Chang, Jen-Shiu Chiang Chiau, Jui-Hsing Chang, Chyong-Hsin Hsu, Chia-Ying Lin, Mary Hsin-Ju Ko, Hung-Chang Lee
Nutrients.2022; 14(23): 5158. CrossRef
Compositional Differences of Meconium Microbiomes of Preterm and Term Infants, and Infants That Developed Necrotizing Enterocolitis or Feeding Intolerance
Hyun Mi Kang, Sol Kim, Seok Hwang-Bo, In Hyuk Yoo, Yu-Mi Seo, Moon Yeon Oh, Soo-Ah Im, Young-Ah Youn
Pathogens.2022; 12(1): 55. CrossRef

Research Support, Non-U.S. Gov'ts

Functional properties of the major outer membrane protein in Stenotrophomonas maltophilia: Yih-Yuan Chen , Han-Chiang Wu , Juey-Wen Lin , Shu-Fen Weng; J. Microbiol. 2015;53(8):535-543. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5202-5

47 View
0 Download
5 Crossref

Abstract

Stenotrophomonas maltophilia is an opportunistic pathogen that is closely associated with high morbidity and mortality in debilitated and immunocompromised individuals. Therefore, to investigate the pathogenesis mechanism is urgently required. However, there are very few studies to evaluate the functional properties of outer membrane protein, which may contribute to the pathogenesis in S. maltophilia. In this study, three abundant proteins in the outer membrane fraction of S. maltophilia were identified by liquid chromatography- tandem mass spectrometry as OmpW1, MopB, and a hypothetical protein. MopB, a member of the OmpA family, was firstly chosen for functional investigation in this study because many OmpA-family proteins are known to be involved in pathogenesis and offer potential as vaccines. Membrane fractionation analyses demonstrated that MopB was indeed the most abundant outer membrane protein (OMP) in S. maltophilia. For functional studies, the mopB mutant of S. maltophilia (SmMopB) was constructed by insertional mutation. MopB deficiency resulted in a change in the protein composition of OMPs and altered the architecture of the outer membrane. The SmMopB strain exhibited reduced cytotoxicity toward L929 fibroblasts and was more sensitive to numerous stresses, including human serum, sodium dodecyl sulfate, and hydrogen peroxide compared with wildtype S. maltophilia. These results suggest that MopB may be a good candidate for the design of vaccines or anti-MopB drugs for controlling serious nosocomial infections of multidrug- resistant S. maltophilia, especially in immunosuppressed patients.

Citations

Citations to this article as recorded by

σ P -NagA-L1/L2 Regulatory Circuit Involved in ΔompA 299-356 -Mediated Increase in β-Lactam Susceptibility in Stenotrophomonas maltophilia
Li-Hua Li, Cheng-Mu Wu, Chia-Lun Chang, Hsin-Hui Huang, Chao-Jung Wu, Tsuey-Ching Yang, Silvia T. Cardona
Microbiology Spectrum.2022;[Epub] CrossRef
Advances in the Microbiology of Stenotrophomonas maltophilia
Joanna S. Brooke
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Recombinant Ax21 protein is a promising subunit vaccine candidate against Stenotrophomonas maltophilia in a murine infection model
Amal Tarek Sarhan, Mohammed Bahey-El-Din, Taha Ibrahim Zaghloul
Vaccine.2021; 39(32): 4471. CrossRef
Intranasal immunization with recombinant outer membrane protein A induces protective immune response against Stenotrophomonas maltophilia infection
Yan Li, Xueping Tang, Zunquan Zhao, Hui Wang, Xin Wang, Xueyi Shang, Peng Liu, Zhihua Kou, Yongqiang Jiang, Yan Li, Daniela Flavia Hozbor
PLOS ONE.2019; 14(4): e0214596. CrossRef
The Major Outer Membrane Protein MopB Is Required for Twitching Movement and Affects Biofilm Formation and Virulence in Two Xylella fastidiosa strains
Hongyu Chen, Prem P. Kandel, Luisa F. Cruz, Paul A. Cobine, Leonardo De La Fuente
Molecular Plant-Microbe Interactions®.2017; 30(11): 896. CrossRef

Proteomic and Functional Analyses of a Novel Porin-like Protein in Xanthomonas oryzae pv. oryzae: Hye-Jee Park , Sang-Won Lee , Sang-Wook Han; J. Microbiol. 2014;52(12):1030-1035. Published online November 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4442-0

43 View
0 Download
13 Crossref

Abstract

Proteomic analysis is a useful technique for postulating and elucidating protein functions. In the present work, a shotgun proteomic analysis was used to identify functions of the PXO_03968 gene (previously known as the ax21) from Xanthomonas oryzae pv. oryzae (Xoo), a causal agent for bacterial blight disease in rice. Structural prediction performed on the protein sequence encoded by PXO_03968 reveals that it encodes a putative porin-like protein, possessing a β-barrel domain with 10 β-strands and a signal peptide at the Nterminus. We renamed the gene as an omp1X (outer membrane protein 1 in Xoo), generated its knock out mutant (XooΔomp1X), and compared the protein expression level in the mutant to that in the wild type. A total of 106 proteins displayed more than 1.5-fold difference in expression between the mutant and the wild type strains. COG analysis revealed that these proteins are involved in cell motility as well as signal transduction. In addition, phenotypic analysis demonstrated that motility and biofilm formation in XooΔomp1X are lower than the wild type. These results provide new insights into the functions of outer membrane proteins in Gram-negative bacteria.

Citations

Citations to this article as recorded by

Stenotrophomonas maltophilia virulence: a current view
Vladimir Mikhailovich, Rustam Heydarov, Danila Zimenkov, Igor Chebotar
Frontiers in Microbiology.2024;[Epub] CrossRef
Molecular-genetic portrait of virulence of Stenotrophomonas maltophilia
Vladimir M. Mikhailovich, Rustam N. Geydarov, Julia A. Bocharova, Igor V. Chebotar
Journal of microbiology, epidemiology and immunobiology.2023; 100(5): 380. CrossRef
p‐Aminobenzoic acid inhibits the growth of soybean pathogen Xanthomonas axonopodis pv. glycines by altering outer membrane integrity
Yong‐Hui Jiang, Ting Liu, Xin‐Chi Shi, Daniela D Herrera‐Balandrano, Mei‐Ting Xu, Su‐Yan Wang, Pedro Laborda
Pest Management Science.2023; 79(10): 4083. CrossRef
Expression and function of clpS and clpA in Xanthomonas campestris pv. campestris
Hsueh-Hsia Lo, Hsiao-Ching Chang, Chao-Tsai Liao, Yi-Min Hsiao
Antonie van Leeuwenhoek.2022; 115(5): 589. CrossRef
Outer Membrane Vesicles: Biogenesis, Functions, and Issues
Rokas Juodeikis, Simon R. Carding
Microbiology and Molecular Biology Reviews.2022;[Epub] CrossRef
Recombinant Ax21 protein is a promising subunit vaccine candidate against Stenotrophomonas maltophilia in a murine infection model
Amal Tarek Sarhan, Mohammed Bahey-El-Din, Taha Ibrahim Zaghloul
Vaccine.2021; 39(32): 4471. CrossRef
Deciphering the functions of the outer membrane porin OprBXo involved in virulence, motility, exopolysaccharide production, biofilm formation and stress tolerance in Xanthomonas oryzae pv. oryzae
Nahee Bae, Hye‐Jee Park, Hanbi Park, Minyoung Kim, Sang‐Wook Han
Molecular Plant Pathology.2018; 19(12): 2527. CrossRef
The Ax21 protein influences virulence and biofilm formation in Stenotrophomonas maltophilia
Shi-qi An, Ji-liang Tang
Archives of Microbiology.2018; 200(1): 183. CrossRef
Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11
Ruping Wang, Huiyong Xu, Yangyang Zhao, Juan Zhang, Gary Y Yuen, Guoliang Qian, Fengquan Liu
AMB Express.2017;[Epub] CrossRef
Systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium Xanthomonas campestris
Sarah Schatschneider, Jessica Schneider, Jochen Blom, Fabien Létisse, Karsten Niehaus, Alexander Goesmann, Frank-Jörg Vorhölter
Microbiology.2017; 163(8): 1117. CrossRef
Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria
Hye-Jee Park, Sang-Wook Han
The Plant Pathology Journal.2017; 33(6): 602. CrossRef
Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
Hye-Jee Park, Chang-Jin Park, Nahee Bae, Sang-Wook Han
The Plant Pathology Journal.2016; 32(3): 266. CrossRef
The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia
Simon Devos, Laurence Van Oudenhove, Stephan Stremersch, Wouter Van Putte, Riet De Rycke, Gonzalez Van Driessche, Jolien Vitse, Koen Raemdonck, Bart Devreese
Frontiers in Microbiology.2015;[Epub] CrossRef

Journal Article

Comparative Proteomics Analysis of Sarcosine Insoluble Outer Membrane Proteins from Clarithromycin Resistant and Sensitive Strains of Helicobacter pylori: Rebecca Smiley , James Bailey , Mahadevan Sethuraman , Norberto Posecion , M. Showkat Ali; J. Microbiol. 2013;51(5):612-618. Published online October 31, 2013; DOI: https://doi.org/10.1007/s12275-013-3029-5

46 View
0 Download
23 Crossref

Abstract

Helicobacter pylori causes disease manifestations in humans including chronic gastric and peptic ulcers, gastric cancer, and lymphoid tissue lymphoma. Increasing rates of H. pylori clarithromycin resistance has led to higher rates of disease development. Because antibiotic resistance involves modifications of outer membrane proteins (OMP) in other Gram-negative bacteria, this study focuses on identification of H. pylori OMP’s using comparative proteomic analyses of clarithromycin-susceptible and -resistant H. pylori strains. Comparative proteomics analyses of isolated sarcosine-insoluble OMP fractions from clarithromycin-susceptible and -resistant H. pylori strains were performed by 1) one dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis protein separation and 2) in-gel digestion of the isolated proteins and mass spectrometry analysis by Matrix Assisted Laser Desorption Ionization-tandem mass spectrometry. Iron-regulated membrane protein, UreaseB, EF-Tu, and putative OMP were down-regulated; HopT (BabB) transmembrane protein, HofC, and OMP31 were up-regulated in clarithromycin-resistant H. pylori. Western blotting and real time PCR, respectively, validated UreaseB subunit and EF-Tu changes at the protein level, and mRNA expression of HofC and HopT. This limited proteomic study provides evidence that alteration of the outer membrane proteins’ profile may be a novel mechanism involved in clarithromycin resistance in H. pylori.

Citations

Citations to this article as recorded by

Testing for Helicobacter pylori in an era of antimicrobial resistance
Audrey N. Schuetz, Elitza S. Theel, Nicolynn C. Cole, Tanner E. Rothstein, Garrett G. Gordy, Robin Patel, Romney M. Humphries
Journal of Clinical Microbiology.2024;[Epub] CrossRef
Bovine lactoferrin inhibits resistant Helicobacter pylori in vitro and protects gastric mucosal injury in vivo
Nannan Wang, Tingting Cai, Xia Liu, Wanping Zhu
International Dairy Journal.2023; 147: 105770. CrossRef
Antibiotic resistance in Helicobacter pylori: From potential biomolecular mechanisms to clinical practice
Yifan Lin, Yongfu Shao, Jianing Yan, Guoliang Ye
Journal of Clinical Laboratory Analysis.2023;[Epub] CrossRef
Multidrug resistance in Helicobacter pylori infection
Raluca Ioana Dascălu, Alexandra Bolocan, Dan Nicolae Păduaru, Alexandru Constantinescu, Magda Mihaela Mitache, Anca Daniela Stoica, Octavian Andronic
Frontiers in Microbiology.2023;[Epub] CrossRef
Antimicrobial resistance patterns and genetic elements associated with the antibiotic resistance of Helicobacter pylori strains from Shanghai
Yixin Liu, Su Wang, Feng Yang, Wenjing Chi, Li Ding, Tao Liu, Feng Zhu, Danian Ji, Jun Zhou, Yi Fang, Jinghao Zhang, Ping Xiang, Yanmei Zhang, Hu Zhao
Gut Pathogens.2022;[Epub] CrossRef
FBPAII and rpoBC, the Two Novel Secreted Proteins Identified by the Proteomic Approach from a Comparative Study between Antibiotic-Sensitive and Antibiotic-Resistant Helicobacter pylori-Associated Gastritis Strains
Suthathip Kittisenachai, Panadda Rojpibulstit, Ratha-Korn Vilaichone, Pornpen Gamnarai, Narumon Phaonakrop, Nuchanart Suealek, Denise Monack
Infection and Immunity.2021;[Epub] CrossRef
Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm
Agnieszka Maszewska, Magdalena Moryl, Junli Wu, Bin Liu, Lu Feng, Antoni Rozalski
Scientific Reports.2021;[Epub] CrossRef
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches
Andreia T. Marques, Jorge M. B. Vítor, Andrea Santos, Mónica Oleastro, Filipa F. Vale
Microbial Genomics .2020;[Epub] CrossRef
Proteomic Applications in Antimicrobial Resistance and Clinical Microbiology Studies

Ehsaneh Khodadadi, Elham Zeinalzadeh, Sepehr Taghizadeh, Bahareh Mehramouz, Fadhil S Kamounah, Ehsan Khodadadi, Khudaverdi Ganbarov, Bahman Yousefi, Milad Bastami, Hossein Samadi Kafil
Infection and Drug Resistance.2020; Volume 13: 1785. CrossRef
Protective effects of Helicobacter pylori membrane vesicles against stress and antimicrobial agents
Benjamin Oliver Murray, Robin Andrew Dawson, Lolwah Mohammad Alsharaf, Jody Anne Winter
Microbiology .2020; 166(8): 751. CrossRef
Proteomics approach to understand bacterial antibiotic resistance strategies
Bo Peng, Hui Li, Xuanxian Peng
Expert Review of Proteomics.2019; 16(10): 829. CrossRef
Helicobacter pylori eradication rates with concomitant and tailored therapy based on 23S rRNA point mutation: A multicenter randomized controlled trial
Sungmoon Ong, Sung Eun Kim, Ji Hyun Kim, Nam Hee Yi, Tae Young Kim, Kyoungwon Jung, Moo In Park, Hwoon‐Yong Jung
Helicobacter.2019;[Epub] CrossRef
Resistance mechanisms ofHelicobacter pyloriand its dual target precise therapy
Yuehua Gong, Yuan Yuan
Critical Reviews in Microbiology.2018; 44(3): 371. CrossRef
The Bifunctional Enzyme SpoT Is Involved in the Clarithromycin Tolerance of Helicobacter pylori by Upregulating the Transporters HP0939, HP1017, HP0497, and HP0471
Xiwen Geng, Wen Li, Zhenghong Chen, Sizhe Gao, Wei Hong, Xiaoran Ge, Guihua Hou, Zhekai Hu, Yabin Zhou, Beini Zeng, Wenjuan Li, Jihui Jia, Yundong Sun
Antimicrobial Agents and Chemotherapy.2017;[Epub] CrossRef
Application of proteomics to the study ofHelicobacter pyloriand implications for the clinic
Giulia Bernardini, Natale Figura, Antonio Ponzetto, Barbara Marzocchi, Annalisa Santucci
Expert Review of Proteomics.2017; 14(6): 477. CrossRef
Novel and Effective Therapeutic Regimens for Helicobacter pylori in an Era of Increasing Antibiotic Resistance
Yi Hu, Yin Zhu, Nong-Hua Lu
Frontiers in Cellular and Infection Microbiology.2017;[Epub] CrossRef
Primary Antibiotic Resistance of Helicobacter pylori in China
Yi Hu, Yin Zhu, Nong-hua Lu
Digestive Diseases and Sciences.2017; 62(5): 1146. CrossRef
Proteomics As a Tool for Studying Bacterial Virulence and Antimicrobial Resistance
Francisco J. Pérez-Llarena, Germán Bou
Frontiers in Microbiology.2016;[Epub] CrossRef
Helicobacter pylori and Antibiotic Resistance, A Continuing and Intractable Problem
Yue Hu, Meng Zhang, Bin Lu, Jinfeng Dai
Helicobacter.2016; 21(5): 349. CrossRef
Global analysis of bacterial membrane proteins and their modifications
Boumediene Soufi, Boris Macek
International Journal of Medical Microbiology.2015; 305(2): 203. CrossRef
Morphological changes in human gastric epithelial cells induced by nuclear targeting of Helicobacter pylori urease subunit A
Jung Hwa Lee, So Hyun Jun, Jung-Min Kim, Seung Chul Baik, Je Chul Lee
Journal of Microbiology.2015; 53(6): 406. CrossRef
High antibiotic resistance rate: A difficult issue forHelicobacter pylorieradication treatment
Mei Zhang
World Journal of Gastroenterology.2015; 21(48): 13432. CrossRef
Proteomic and functional analyses of a novel porin-like protein in Xanthomonas oryzae pv. oryzae
Hye-Jee Park, Sang-Won Lee, Sang-Wook Han
Journal of Microbiology.2014; 52(12): 1030. CrossRef

Research Support, Non-U.S. Gov'ts

Identification of Conserved Surface Proteins as Novel Antigenic Vaccine Candidates of Actinobacillus pleuropneumoniae: Xiabing Chen , Zhuofei Xu , Lu Li , Huanchun Chen , Rui Zhou; J. Microbiol. 2012;50(6):978-986. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2214-2

38 View
0 Download
15 Scopus

Abstract: Actinobacillus pleuropneumoniae is an important swine respiratory pathogen causing great economic losses worldwide. Identification of conserved surface antigenic proteins is helpful for developing effective vaccines. In this study, a genome-wide strategy combined with bioinformatic and experimental approaches, was applied to discover and characterize surface-associated immunogenic proteins of A. pleuropneumoniae. Thirty nine genes encoding outer membrane proteins (OMPs) and lipoproteins were identified by comparative genomics and gene expression profiling as beinghighly conserved and stably transcribed in the different serotypes of A. pleuropneumoniae reference strains. Twelve of these conserved proteins were successfully expressed in Escherichia coli and their immunogenicity was estimated by homologous challenge in the mouse model, and then three of these proteins (APJL_0126, HbpA and OmpW) were further tested in the natural host (swine) by homologous and heterologous challenges. The results showed that these proteins could induce high titers of antibodies, but vaccination with each protein individually elicited low protective immunity against A. pleuropneumoniae. This study gives novel insights into immunogenicity of the conserved OMPs and lipoproteins of A. pleuropneumoniae. Although none of the surface proteins characterized in this study could individually induce effective protective immunity against A. pleuropneumoniae, they are potential candidates for subunit vaccines in combination with Apx toxins.

Structural and Functional Importance of Outer Membrane Proteins in Vibrio cholerae Flagellum: Wasimul Bari , Kang-Mu Lee , Sang Sun Yoon; J. Microbiol. 2012;50(4):631-637. Published online August 25, 2012; DOI: https://doi.org/10.1007/s12275-012-2116-3

41 View
0 Download
16 Scopus

Abstract: Vibrio cholerae has a sheath-covered monotrichous flagellum that is known to contribute to virulence. Although the structural organization of the V. cholerae flagellum has been extensively studied, the involvement of outer membrane proteins as integral components in the flagellum still remains elusive. Here we show that flagella produced by V. cholerae O1 El Tor strain C6706 were two times thicker than those from two other Gram-negative bacteria. A C6706 mutant strain (SSY11) devoid of two outer membrane proteins (OMPs), OmpU and OmpT, produced thinner flagella. SSY11 showed significant defects in the flagella-mediated motility as compared to its parental strain. Moreover, increased shedding of the flagella-associated proteins was observed in the culture supernatant of SSY11. This finding was also supported by the observation that culture supernatants of the SSY11 strain induced the production of a significantly higher level of IL-8 in human colon carcinoma HT29 and alveolar epithelial A549 cells than those of the wild-type C6706 strain. These results further suggest a definite role of these two OMPs in providing the structural integrity of the V. cholerae flagellum as part of the surrounding sheath.

Acinetobacter baumannii Outer Membrane Protein A Modulates the Biogenesis of Outer Membrane Vesicles: Dong Chan Moon , Chul Hee Choi , Jung Hwa Lee , Chi-Won Choi , Hye-Yeon Kim , Jeong Soon Park , Seung Il Kim , Je Chul Lee; J. Microbiol. 2012;50(1):155-160. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1589-4

31 View
0 Download
102 Scopus

Abstract: Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo growth, but the biogenesis mechanism by which A. baumannii produces OMVs remains undefined. Outer membrane protein A of A. baumannii (AbOmpA) is a major protein in the outer membrane and the C-terminus of AbOmpA interacts with diaminopimelate of peptidoglycan. This study investigated the role of AbOmpA in the biogenesis of A. baumannii OMVs. Quantitative and qualitative approaches were used to analyze OMV biogenesis in A. baumannii ATCC 19606T and an isogenic ΔAbOmpA mutant. OMV production was significantly increased in the ΔAbOmpA mutant compared to wild-type bacteria as demonstrated by quantitation of proteins and lipopolysaccharides (LPS) packaged in OMVs. LPS profiles prepared from OMVs from wild-type bacteria and the ΔAbOmpA mutant had identical patterns, but proteomic analysis showed different protein constituents in OMVs from wild-type bacteria compared to the ΔAbOmpA mutant. In conclusion, AbOmpA influences OMV biogenesis by controlling OMV production and protein composition.

Immunostimulatory Activity of Dendritic Cells Pulsed with Carbonic Anhydrase IX and Acinetobacter baumannii Outer Membrane Protein A for Renal Cell Carcinoma: Bo Ra Kim , Eun Kyoung Yang , Sun Hee Kim , Dong Chan Moon , Hwa Jung Kim , Je Chul Lee , Duk Yoon Kim; J. Microbiol. 2011;49(1):115-120. Published online March 3, 2011; DOI: https://doi.org/10.1007/s12275-011-1037-x

27 View
0 Download
5 Scopus

Abstract: Dendritic cell (DC)-based immunotherapy is a potent therapeutic modality for treating renal cell carcinoma (RCC), but development of antigens specific for tumor-targeting and anti-tumor immunity is of great interest for clinical trials. The present study investigated the ability of DCs pulsed with a combination of carbonic anhydrase IX (CA9) as an RCC-specific biomarker and Acinetobacter baumannii outer membrane protein A (AbOmpA) as an immunoadjuvant to induce anti-tumor immunity against murine renal cell carcinoma (RENCA) in a murine model. Murine bone-marrow-derived DCs pulsed with a combination of RENCA lysates and AbOmpA were tested for their capacity to induce DC maturation and T cell responses in vitro. A combination of RENCA lysates and AbOmpA up-regulated the surface expression of co-stimulatory molecules, CD80 and CD86, and the antigen presenting molecules, major histocompatibility (MHC) class I and class II, in DCs. A combination of RENCA lysates and AbOmpA also induced interleukin-12 (IL-12) production in DCs. Next, the immunostimulatory activity of DCs pulsed with a combination of CA9 and AbOmpA was determined. A combination of CA9 and AbOmpA up-regulated the surface expression of co-stimulatory molecules and antigen presenting molecules in DCs. DCs pulsed with a combination of CA9 and AbOmpA effectively secreted IL-12 but not IL-10. These cells interacted with T cells and formed clusters. DCs pulsed with CA9 and AbOmpA elicited the secretion of interferon-γ and IL-2 in T cells. In conclusion, a combination of CA9 and AbOmpA enhanced the immunostimulatory activity of DCs, which may effectively induce anti-tumor immunity against human RCC.

Overexpression of Outer Membrane Protein OprT and Increase of Membrane Permeability in phoU Mutant of Toluene-Tolerant Bacterium Pseudomonas putida GM730: Kyunghee Lee , Juna Jung , Kwang Kim , Dongwon Bae , Dongbin Lim; J. Microbiol. 2009;47(5):557-562. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0105-y

38 View
0 Download
5 Scopus

Abstract: Eight toluene-sensitive mutants were previously isolated from the toluene-tolerant bacterium Pseudomonas putida GM730. One of these mutants was TOS6, in which Tn5 had been inserted into phoU. Susceptibility to multiple antibiotics, as well as toluene sensitivity, was increased in the phoU mutant of P. putida GM730. We compared the outer membrane proteins from the phoU mutant and wild-type via two-dimensional gel electrophoresis. A 45 kDa protein was dramatically overexpressed as the result of phoU inactivation, and this protein was identified by peptide mass fingerprinting and microsequencing as a conserved hypothetical protein consisting of 414 amino acids. The protein, designated as OprT, harbors a signal sequence and extended β-sheets, both of which are features common to the bacterial porins. The rate of ethidium bromide accumulation in TOS6 was higher than in GM730, which indicates that the TOS6 membranes may be more permeable to ethidium bromide than are the membranes of GM730. We propose that the toluene sensitivity and increased antibiotic susceptibility observed in the phoU mutant may be attributable to increased membrane permeability.

Journal Article

Proteomic Analysis of Outer Membrane Proteins from Acinetobacter baumannii DU202 in Tetracycline Stress Condition: Sung-Ho Yun , Chi-Won Choi , Soon-Ho Park , Je Chul Lee , Sun-Hee Leem , Jong-Soon Choi , Soohyun Kim , Seung Il Kim; J. Microbiol. 2008;46(6):720-727. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0202-3

27 View
0 Download
53 Scopus

Abstract: Acinetobacter baumannii readily developed antimicrobial resistance to clinically available antibiotics. A. baumannii DU202 is a multi-drug resistant strain, and is highly resistant to tetracycline (MIC>1,024μg/ml). The surface proteome of A. baumannii DU202 in response to the sub-minimal inhibitory concentration (subMIC) of tetracycline was analyzed by 2-DE/MS-MS and 1-DE/LC/MS-MS to understand the pathways that form barriers for tetracycline. Membrane expression of major outer membrane proteins (Omps) was significantly decreased in response to the subMIC of tetracycline. These Omps with sizes of 38, 32, 28, and 21 kDa were identified as OmpA38, OmpA32, CarO, and OmpW, respectively. However, transcription level of these Omps was not significantly changed. 1-DE/LC/MS-MS analysis of secreted proteins showed that OmpA38, CarO, OmpW, and other Omps were increasingly secreted at tetracycline condition. This result suggests that A. baumannii actively regulates the membrane expression and the secretion of Omps to overcome antibiotic stress condition.

Research Support, Non-U.S. Gov't

Anti-Tumor Activity of Acinetobacter baumannii Outer Membrane Protein A on Dendritic Cell-Based Immunotherapy against Murine Melanoma: Jun Sik Lee , Jung Wook Kim , Chul Hee Choi , Won Kee Lee , Hae Young Chung , Je Chul Lee; J. Microbiol. 2008;46(2):221-227. Published online June 11, 2008; DOI: https://doi.org/10.1007/s12275-008-0052-z

36 View
0 Download
14 Scopus

Abstract: Acinetobacter baumannii outer membrane protein A (AbOmpA) is a major surface protein that is an important pathogen-associated molecular pattern. Based on our previous findings that AbOmpA induced the phenotypic maturation of dendritic cells (DCs) and drove the Th1 immune response in vitro, we investigated the therapeutic efficacy of AbOmpA-pulsed DC vaccines in a murine melanoma model. The surface expression of co-stimulatory molecules (CD80 and CD86) and major histocompatibility complex class I and II molecules was higher in DCs pulsed with AbOmpA alone or with a combination of B16F10 cell lysates than that of DCs pulsed with B16F10 cell lysates. AbOmpA stimulated the maturation of murine splenic DCs in vivo. In a therapeutic model of murine melanoma, AbOmpA-pulsed DCs significantly retarded tumor growth and improved the survival of tumor-bearing mice. AbOmpA-pulsed DCs significantly enhanced CD8+, interleukin-2+ T cells and CD4+, interferon-γ+ T cells in tumor-bearing mice. These results provide evidence that AbOmpA may be therapeutically useful in adjuvant DC immunotherapy against poorly immunogenic melanoma without tumor-specific antigens.

Journal Articles

Outer Membrane Protein H for Protective Immunity Against Pasteurella multocida: Jeongmin Lee , Young Bong Kim , Moosik Kwon; J. Microbiol. 2007;45(2):179-184.; DOI: https://doi.org/2514 [pii]

40 View
0 Download

Abstract: Pasteurella multocida, a Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. For the development of recombinant subunit vaccine against P. multocida, we cloned and analyzed the gene for outer membrane protein H (ompH) from a native strain of Pasteurella multocida in Korea. The OmpH had significant similarity in both primary and secondary structure with those of other serotypes. The full-length, and three short fragments of ompH were expressed in E. coli and the recombinant OmpH proteins were purified, respectively. The recombinant OmpH proteins were antigenic and detectable with antisera produced by either immunization of commercial vaccine for respiratory disease or formalin-killed cell. Antibodies raised against the full-length OmpH provided strong protection against P. multocida, however, three short fragments of recombinant OmpHs, respectively, showed slightly lower protection in mice challenge. The recombinant OmpH might be a useful vaccine candidate antigen for P. multocida.

Immunization with Major Outer Membrane Protein of Vibrio vulnificus Elicits Protective Antibodies in a Murine Model: Cho-Rok Jung , Min-Jung Park , Moon-Soo Heo; J. Microbiol. 2005;43(5):437-442.; DOI: https://doi.org/2278 [pii]

36 View
0 Download

Abstract: Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on 13% SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG , which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice. The group of mice immunized with MOMP and Alum showed higher levels of IgG2b than the group immunized with only MOMP. Vaccination with MOMP resulted in protective antibodies in the mouse infection experiment.

First
Prev
Page of 1
Next
Last

Search