Journal of Microbiology

Journal Article

Effects of Nicotine on the Growth and Protein Expression of Porphyromonas gingivalis: Orson Baek , Weidong Zhu , Hyeong C. Kim Kim , Seok-Woo Lee Lee; J. Microbiol. 2012;50(1):143-148. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1212-8

40 View
0 Download
19 Crossref

Tobacco smoking is considered one of the most significant environmental risk factors for destructive periodontal disease. The effect of smoking on periodontopathic microbiota has not yet been elucidated, as previous studies failed to identify a concrete relationship between periodontopathic microorganisms and smoking. However, it is likely that smoking, as an environmental stress factor, may affect the behavior of dental plaque microorganisms, ultimately leading to alteration of the host-parasite interaction. The goal of this study was to examine the effect of nicotine, a major component of tobacco, on the growth and protein expression of the crucial periodontal pathogen Porphyromonas gingivalis. The growth of P. gingivalis 381 was measured after bacterial cells were cultivated in liquid broth containing various nicotine concentrations. First, P. gingivalis cells were allowed to grow in the presence of a single dose of nicotine (the single exposure protocol) at 0, 1, 2, 4, and 8 mg/L, respectively. Second, P. gingivalis cells were exposed to five consecutive doses of nicotine (the multiple exposure protocol) at 0, 1, 2, and 4 mg/L, respectively. Bacterial growth was measured by optical density and protein expression was analyzed by SDS-PAGE and 2-D gel electrophoresis. In the single nicotine exposure protocol, it was observed that the growth of P. gingivalis 381 was inhibited by nicotine in a dose-dependent manner. In the multiple nicotine exposure protocol, the growth rate of P. gingivalis increased with each subsequent nicotine exposure, even though bacterial growth was also inhibited in a dose dependent fashion. SDS-PAGE and 2-D gel electrophoresis analyses revealed a minor change in the pattern of protein expression, showing differences in proteins with low molecular weights (around 20 kDa) on exposure to nicotine. The results of this study suggest that nicotine exerts an inhibitory effect on the growth of P. gingivalis, and has a potential to modulate protein expression in P. gingivalis.

Citations

Citations to this article as recorded by

Smoking Impacts Alzheimer’s Disease Progression Through Oral Microbiota Modulation
Xingzhu Dai, Rui Liang, Manqiong Dai, Xiaoyu Li, Wanghong Zhao
Molecular Neurobiology.2025; 62(1): 19. CrossRef
Nicotine promotes pathogenic bacterial growth and biofilm formation in peri-implant
Rong Hu, Huifen Qian, Xiangyun Wang, Bei Peng, Dahai Huang
Journal of Medical Microbiology .2024;[Epub] CrossRef
Identification of habit specific bacteria in human saliva through Next-Generation Sequencing
Shubham Yadav, Vijay Tripathi, Vaibhav Saran
Forensic Science International.2023; 353: 111871. CrossRef
The influence of smoking on the periodontal biome. A review.
Luminița Lazăr, Timea Dakó, Andreea Cozma, Ana-Petra Lazăr
Acta Stomatologica Marisiensis Journal.2022; 5(1): 6. CrossRef
An in vitro comparison of four antibacterial agents with and without nicotine and their effects on human gingival fibroblasts
Chandni Batra, Marwa Alalshaikh, Richard L. Gregory, L. Jack Windsor, Steven B. Blanchard, Yusuke Hamada
Journal of Periodontology.2022;[Epub] CrossRef
A Systematic Review of the Literature Examining the Effects of Cigarette Smoke and e-Cigarette Vapor on the Virulence of Human Pathogenic Bacteria
Kamal Bagale, Ritwij Kulkarni
International Journal of Environmental Research and Public Health.2022; 19(19): 12518. CrossRef
The Impact of Smoking on Subgingival Plaque and the Development of Periodontitis: A Literature Review
Jiaxin Zhang, Jialu Yu, Jinge Dou, Pingyue Hu, Qiang Guo
Frontiers in Oral Health.2021;[Epub] CrossRef
Environmental factors and periodontal microbiome
Nurcan Buduneli
Periodontology 2000.2021; 85(1): 112. CrossRef
The Synergistic Effect of Nicotine and Staphylococcus aureus on Peri-Implant Infections
Yao Hu, Wen Zhou, Chengguang Zhu, Yujie Zhou, Qiang Guo, Xiaoyu Huang, Bina Yang, Biao Ren, Lei Cheng
Frontiers in Bioengineering and Biotechnology.2021;[Epub] CrossRef
A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing
Roland Wirth, Gergely Maróti, Róbert Mihók, Donát Simon-Fiala, Márk Antal, Bernadett Pap, Anett Demcsák, Janos Minarovits, Kornél L. Kovács
Journal of Oral Microbiology.2020; 12(1): 1773067. CrossRef
The Impact of Smoking on Subgingival Microflora: From Periodontal Health to Disease
Yaling Jiang, Xuedong Zhou, Lei Cheng, Mingyun Li
Frontiers in Microbiology.2020;[Epub] CrossRef
The effect of cigarette smoking onPorphyromonas gingivalis, a crucial periodontal pathogen
Junghoon Shin, Seok-Woo Lee
Oral Biology Research.2019; 43(1): 17. CrossRef
Effect of tobacco on periodontal disease and oral cancer
Yixin Zhang, Jinxiu He, Bing He, Ruijie Huang, Mingyun Li
Tobacco Induced Diseases.2019;[Epub] CrossRef
Smoking and periodontal microorganisms
Takashi Hanioka, Manabu Morita, Tatsuo Yamamoto, Koji Inagaki, Pao-Li Wang, Hiroshi Ito, Toshiya Morozumi, Toru Takeshita, Nao Suzuki, Hideo Shigeishi, Masaru Sugiyama, Kouji Ohta, Toru Nagao, Nobuhiro Hanada, Miki Ojima, Hiroshi Ogawa
Japanese Dental Science Review.2019; 55(1): 88. CrossRef
Observational cross-sectional study of Trichomonas tenax in patients with periodontal disease attending a Chilean university dental clinic
Casandra Bracamonte-Wolf, Patricio R. Orrego, Christian Muñoz, Daniel Herrera, Joel Bravo, Jorge Gonzalez, Héctor Varela, Alejandro Catalán, Jorge E. Araya
BMC Oral Health.2019;[Epub] CrossRef
Dynamic metabolomic responses ofEscherichia colito nicotine stress
Lijian Ding, Juanjuan Chen, Jianding Zou, Limin Zhang, Yangfang Ye
Canadian Journal of Microbiology.2014; 60(8): 547. CrossRef
Detection and Quantification of Periodontal Pathogens in Smokers and Never‐Smokers With Chronic Periodontitis by Real‐Time Polymerase Chain Reaction
Mariana R. Guglielmetti, Ecinele F. Rosa, Daniele S. Lourenção, Gislene Inoue, Elaine F. Gomes, Giorgio De Micheli, Fausto Medeiros Mendes, Rosário D.C. Hirata, Mario H. Hirata, Claudio M. Pannuti
Journal of Periodontology.2014; 85(10): 1450. CrossRef
Periodontal pathogens and associated factors in aggressive periodontitis: results 5–17 years after active periodontal therapy
Amelie Meyer‐Bäumer, Sigrun Eick, Christian Mertens, Lorenz Uhlmann, Daniel Hagenfeld, Peter Eickholz, Ti‐Sun Kim, Raluca Cosgarea
Journal of Clinical Periodontology.2014; 41(7): 662. CrossRef
Proteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine
K. Cogo, A. de Andrade, C. A. Labate, C. C. Bergamaschi, L. A. Berto, G. C. N. Franco, R. B. Gonçalves, F. C. Groppo
Journal of Periodontal Research.2012; 47(6): 766. CrossRef

First
Prev
Page of 1
Next
Last

Search