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- Rotavirus-mediated alteration of gut microbiota and its correlation with physiological characteristics in neonatal calves
- Ja-Young Jang , Suhee Kim , Min-Sung Kwon , Jieun Lee , Do-Hyeon Yu , Ru-Hui Song , Hak-Jong Choi , Jinho Park
- J. Microbiol. 2019;57(2):113-121. Published online November 19, 2018
- DOI: https://doi.org/10.1007/s12275-019-8549-1
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Abstract
- Diarrhea is a fatal disease to neonatal calves, and rotavirus is
the main pathogen associated with neonatal calf diarrhea.
Although previous studies have reported that the gut microbiota
is changed in calves during diarrhea, less is known
about whether rotavirus infection alters the structure of the
gut microbiota. Here, we characterized fecal microbial communities
and identified possible relationships between the
gut microbiota profiles and physiological parameters. Five
fecal specimens of rotavirus-infected calves from 1 to 30 days
after birth and five fecal specimens of age-matched healthy
calves were used for the microbial community analysis using
the Illumina MiSeq sequencer. Rotavirus infection was
associated with reduced rotavirus infection significantly reduced
the richness and diversity of the bacterial community.
Weighted unique fraction metric analysis exhibited significant
differences in community membership and structure between
healthy and rotavirus-infected calves. Based on relative abundance
analysis and linear discriminant analysis effect size, we
found that the representative genera from Lactobacillus, Subdoligranulum,
Blautia, and Bacteroides were closely related to
healthy calves, while the genera Escherichia and Clostridium
were closely affiliated to rotavirus-infected calves. Furthermore,
canonical correlation analysis and Pearson correlation
coefficient results revealed that the increased relative abundances
of Lactobacillus, Subdoligranulum, and Bacteroides
were correlated with normal levels of physiological characteristics
such as white blood cells, blood urea nitrogen, serum
amyloid protein A, and glucose concentration in serum. These
results
suggest that rotavirus infection alters the structure of the gut microbiota, correlating changes in physiological
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