MSK
Search
- Page Path
- HOME > Search
Journal Article
- Silencing the cleavage factor CFIm25 as a new strategy to control Entamoeba histolytica parasite
- Juan David Ospina-Villa , Nancy Guillén , Cesar Lopez-Camarillo , Jacqueline Soto-Sanchez , Esther Ramirez-Moreno , Raul Garcia-Vazquez , Carlos A. Castañon-Sanchez , Abigail Betanzos , Laurence A. Marchat
- J. Microbiol. 2017;55(10):783-791. Published online September 28, 2017
- DOI: https://doi.org/10.1007/s12275-017-7259-9
- 67 View
- 0 Download
- 10 Crossref
-
Abstract
- The 25 kDa subunit of the Clevage Factor Im (CFIm25) is an essential factor for messenger RNA polyadenylation in human cells. Therefore, here we investigated whether the homologous protein of Entamoeba histolytica, the protozoan responsible for human amoebiasis, might be considered as a biochemical target for parasite control. Trophozoites were cultured with bacterial double-stranded RNA molecules targeting the EhCFIm25 gene, and inhibition of mRNA and protein expression was confirmed by RT-PCR and Western blot assays, respectively. EhCFIm25 silencing was associated with a significant acceleration of cell proliferation and cell death. Moreover, trophozoites appeared as larger and multinucleated cells. These morphological changes were accompanied by a reduced mobility, and erythrophagocytosis was significantly diminished. Lastly, the knockdown of EhCFIm25 affected the poly(A) site selection in two reporter genes and revealed that EhCFIm25 stimulates the utilization of downstream poly(A) sites in E. histolytica mRNA. Overall, our data confirm that targeting the polyadenylation process represents an interesting strategy for controlling parasites, including E. histolytica. To our best knowledge, the present study is the first to have revealed the relevance of the cleavage factor CFIm25 as a biochemical target in parasites.
-
Citations
Citations to this article as recorded by
- Comparative genomics and interactomics of polyadenylation factors for the prediction of new parasite targets: Entamoeba histolytica as a working model
Rodolfo Gamaliel Avila-Bonilla, Jorge Antonio Velazquez-Guzman, Eimy Itzel Reyes-Zepeda, Jorge Luis Gutierrez-Avila, César A Reyes-López, Alondra Cisneros-Sarabia, Emma Saavedra, Angel Lopéz-Sandoval, Esther Ramírez-Moreno, César López-Camarillo, Laurence
Bioscience Reports.2023;[Epub] CrossRef - CFI 25 Subunit of Cleavage Factor I is Important for Maintaining the Diversity of 3ʹ UTR Lengths in Arabidopsis thaliana (L.) Heynh.
Xiaojuan Zhang, Mika Nomoto, Marta Garcia-León, Naoki Takahashi, Mariko Kato, Kei Yura, Masaaki Umeda, Vicente Rubio, Yasuomi Tada, Tsuyoshi Furumoto, Takashi Aoyama, Tomohiko Tsuge
Plant and Cell Physiology.2022; 63(3): 369. CrossRef - Unraveling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica through proteomic analysis
América Itzallana Salgado‐Martínez, Rodolfo Gamaliel Avila‐Bonilla, Esther Ramírez‐Moreno, Carlos Alberto Castañón‐Sánchez, César López‐Camarillo, Laurence A. Marchat
FEBS Open Bio.2021; 11(10): 2819. CrossRef - Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation
Tingting Weng, Jingjing Huang, Eric J. Wagner, Junsuk Ko, Minghua Wu, Nancy E. Wareing, Yu Xiang, Ning-Yuan Chen, Ping Ji, Jose G. Molina, Kelly A. Volcik, Leng Han, Maureen D. Mayes, Michael R. Blackburn, Shervin Assassi
Journal of Experimental Medicine.2020;[Epub] CrossRef - mRNA Polyadenylation Machineries in Intestinal Protozoan Parasites
Juan David Ospina‐Villa, Brisna Joana Tovar‐Ayona, César López‐Camarillo, Jacqueline Soto‐Sánchez, Esther Ramírez‐Moreno, Carlos A. Castañón‐Sánchez, Laurence A. Marchat
Journal of Eukaryotic Microbiology.2020; 67(3): 306. CrossRef - Los aptámeros como novedosa herramienta diagnóstica y terapéutica y su potencial uso en parasitología
Juan David Ospina
Biomédica.2020; 40(Supl. 1): 148. CrossRef - Target identification and intervention strategies against amebiasis
Shruti Nagaraja, Serge Ankri
Drug Resistance Updates.2019; 44: 1. CrossRef - Advances on Aptamers against Protozoan Parasites
Juan David Ospina-Villa, César López-Camarillo, Carlos A. Castañón-Sánchez, Jacqueline Soto-Sánchez, Esther Ramírez-Moreno, Laurence A. Marchat
Genes.2018; 9(12): 584. CrossRef - Targeting the polyadenylation factor EhCFIm25 with RNA aptamers controls survival in Entamoeba histolytica
Juan David Ospina-Villa, Alexandre Dufour, Christian Weber, Esther Ramirez-Moreno, Absalom Zamorano-Carrillo, Nancy Guillen, César Lopez-Camarillo, Laurence A. Marchat
Scientific Reports.2018;[Epub] CrossRef - Life and Death of mRNA Molecules in Entamoeba histolytica
Jesús Valdés-Flores, Itzel López-Rosas, César López-Camarillo, Esther Ramírez-Moreno, Juan D. Ospina-Villa, Laurence A. Marchat
Frontiers in Cellular and Infection Microbiology.2018;[Epub] CrossRef
- Comparative genomics and interactomics of polyadenylation factors for the prediction of new parasite targets: Entamoeba histolytica as a working model
Published Erratum
- Erratum to: UBCG2: Up‑to‑Date Bacterial Core Genes and Pipeline for Phylogenomic Analysis
- Jihyeon Kim , Seong-In Na , Dongwook Kim , Jongsik Chun
- J. Microbiol. 2023;61(10):937-937.
- DOI: https://doi.org/10.1007/s12275-023-00074-0
- 64 View
- 0 Download
- Quantitative Analysis of Expressed Genes in Aspergillus Oryzae by Sequencing 3'-directed cDNA Clones
- Hwang, Hyun Ah , Lee, Dong Whan , Kim, Jong Hwa , Lee, Tae Kyoo , Yang, Moon Sik , Chae, Keon Sang
- J. Microbiol. 1998;36(2):111-117.
- 55 View
- 0 Download
-
Abstract
- Sequence analysis of randomly selected 3'-directed cKNA clones has been known to be one of the most powerful methods of examining the genes highly expressed in a tissue or cell type. We constructed a 3'-directed cDNA libraty from Aspergillus oryzae mycelia, and sequenced 345 randomly selected 3'-directed cDNA clones. Determined nucleotide sequences, not shorter than 30nt, were compared with one other to generate gene signatures (GSs) and were then compared with GenBank entries to analyze sequence similarity to known genes. A GS for the most highly expressed gene appeared six times, one GS five times, five GSs four times, five GSs three times and 22 GSs twice. In total, 324 clones yielded 268 GSs consisting of 34 redundant GSs appeaning at least twice and 234 solitary ones. Forty-three GSs showed similarities ranging from 60% to 99% with known sequences from Genbank. A considerable number of A. oryzae GSs mateched those obtained from the sexual structures of A. nidulans suggests that A. oryzae may not be phylogentically distant from A. nidulans and that A. oryzae may have a sexual life cycle from the ancient period.
First
Prev
TOP
