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- A Genome-Wide Identification of Genes Potentially Associated with Host Specificity of Brucella Species
- Kyung Mo Kim , Kyu-Won Kim , Samsun Sung , Heebal Kim
- J. Microbiol. 2011;49(5):768-775. Published online November 9, 2011
- DOI: https://doi.org/10.1007/s12275-011-1084-3
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Abstract
- Brucella species are facultative intracellular pathogenic α-Proteobacteria that can cause brucellosis in humans and domestic animals. The clinical and veterinary importance of the bacteria has led to well established studies on the molecular mechanisms of Brucella infection of host organisms. However, to date, no genome-wide study has scanned for genes related to the host specificity of Brucella spp. The majority of bacterial genes related to specific environmental adaptations such as host specificity are well-known to have evolved under positive selection pressure. We thus detected signals of positive selection for individual orthologous genes among Brucella genomes and identified genes related to host specificity. We first determined orthologous sets from seven completely sequenced Brucella genomes using the Reciprocal Best Hits (RBH). A maximum likelihood analysis based on the branch-site test was accomplished to examine the presence of positive selection signals, which was subsequently confirmed by phylogenetic analysis. Consequently, 12 out of 2,033 orthologous genes were positively selected by specific Brucella lineages, each of which belongs to a particular animal host. Extensive literature reviews revealed that half of these computationally identified genes are indeed involved in Brucella host specificity. We expect that this genome-wide approach based on positive selection may be reliably used to screen for genes related to environmental adaptation of a particular species and that it will provide a set of appropriate candidate genes.
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Citations
Citations to this article as recorded by
- Clade-specific positive selection on a developmental gene: BRANCHLESS TRICHOME and the evolution of stellate trichomes in Physaria (Brassicaceae)
Abigail R. Mazie, David A. Baum
Molecular Phylogenetics and Evolution.2016; 100: 31. CrossRef - Identification of Recombination and Positively Selected Genes in Brucella
Udayakumar S. Vishnu, Jagadesan Sankarasubramanian, Jayavel Sridhar, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
Indian Journal of Microbiology.2015; 55(4): 384. CrossRef - Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
Miryan Margot Sánchez-Jiménez, Juan Pablo Isaza, Juan F. Alzate, Martha Olivera-Angel
Genomics.2015; 106(1): 43. CrossRef - Complete Genome Sequence of Brucella canis Strain 118, a Strain Isolated from Canine
Guangjun Gao, Jing Li, Tiefeng Li, Zhengfang Zhang, Liping Wang, Xitong Yuan, Yufei Wang, Jie Xu, Yuehua Ke, Liuyu Huang, Dali Wang, Zeliang Chen, Xingran Xu
Journal of Bacteriology.2012; 194(23): 6680. CrossRef - Complete Genome Sequence of Brucella canis BCB018, a Strain Isolated from a Human Patient
Yufei Wang, Yuehua Ke, Qing Zhen, Xitong Yuan, Jie Xu, Yefeng Qiu, Zhoujia Wang, Tiefeng Li, Dali Wang, Liuyu Huang, Zeliang Chen
Journal of Bacteriology.2012; 194(23): 6697. CrossRef
- Clade-specific positive selection on a developmental gene: BRANCHLESS TRICHOME and the evolution of stellate trichomes in Physaria (Brassicaceae)
- Construction of Multiple Mutant Strains by Mating Procedures for the Cloning of pmn and pmb Genes Encoding Amino Acid Permeases in Neurospora crassa
- Han, Hyo Young , Min, Kyung Hee
- J. Microbiol. 1995;33(2):142-145.
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Abstract
- The pmb gene encoding a basic amino acid transport protein in Neurospora crassa could be cloned by using a mutant strain defective in pmb gene as a host strain, using a negative selection on the media containing amino acid analogue canavanine. To select positive transformants of the genes for cloning, an auxotrophic marker (his-2) was added to a pmb mutant strain by mating ; a triple mutant (pmn : pmb : his-2) was constructued by crossing a strain defective in basic amino acid transport system (# 1683-bat um 535 "A") to a double mutant strain defective in neutral amino acid transport and histidine production (mitr6r : his-2 "a"). Crossing was performed on synthetic crossing (SC) media containing histidine. The pmn : pmb and pmn :pmb : his-2 strains were selected among the progeny colonies from crosses on plates containing 50㎍/㎖ para-fluoro-phenylalanine (PFPA), 200㎍/㎖ canavanine, and 500㎍/㎖ histidine. The selected colonies were cultured on minimal media with or without histidine for discarding pmn : pmb strain, because the pmn : pmb : his -2 strain grows only on histidine containing media. The pmn :pmb : his-2 strain selected can be used as a host strain for the cloning of the pmb and the pmn genes from a Neurospora genomic library by means of positive selections.
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