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- Rapid determination of carbapenem resistance by low-cost colorimetric methods: Propidium Iodide and alamar blue staining
- Jiyoon Choi , Jiwon Baek , Daehyuk Kweon , Kwan Soo Ko , Hyunjin Yoon
- J. Microbiol. 2020;58(5):415-421. Published online March 28, 2020
- DOI: https://doi.org/10.1007/s12275-020-9549-x
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- 4 Web of Science
- 4 Crossref
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Abstract
-
Carbapenems are a class of β-lactam antibiotics with a broad
antimicrobial activity spectrum. Owing to their sturdy structures
resistant to most β-lactamases, they have been regarded
as one of the last-resort antibiotics for combating multidrugresistant
bacterial infections. However, the emergence of carbapenem
resistance increases predominantly in nosocomial
pathogens. To prevent spread of carbapenem resistance in
early stages, it is imperative to develop rapid diagnostic tests
that will substantially reduce the time and cost in determining
carbapenem resistance. Thus, we devised a staining-based
diagnostic method applicable to three different Gram-negative
pathogens of Acinetobacter baumannii, Escherichia coli,
and Klebsiella pneumoniae, all with the high potential to develop
carbapenem resistance. Regardless of the resistance mechanisms
presented by bacterial species and strains, double
staining with propidium iodide (PI) and alamar blue (AB)
identified resistant bacteria with an average sensitivity of
95.35%, 7 h after imipenem treatments in 343 clinical isolates.
Among the three species tested, A. baumannii showed the
highest diagnostic sensitivity of 98.46%. The PI and ABmediated
staining method could be a promising diagnostic
method
with high-throughput efficacy and low cost. -
Citations
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