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- Envelope‑Stress Sensing Mechanism of Rcs and Cpx Signaling Pathways in Gram‑Negative Bacteria
- Seung-Hyun Cho , Kilian Dekoninck , Jean-Francois Collet
- J. Microbiol. 2023;61(3):317-329. Published online March 9, 2023
- DOI: https://doi.org/10.1007/s12275-023-00030-y
- 54 View
- 0 Download
- 11 Web of Science
- 9 Crossref
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Abstract
- The global public health burden of bacterial antimicrobial resistance (AMR) is intensified by Gram-negative bacteria,
which have an additional membrane, the outer membrane (OM), outside of the peptidoglycan (PG) cell wall. Bacterial twocomponent
systems (TCSs) aid in maintaining envelope integrity through a phosphorylation cascade by controlling gene
expression through sensor kinases and response regulators. In Escherichia coli, the major TCSs defending cells from envelope
stress and adaptation are Rcs and Cpx, which are aided by OM lipoproteins RcsF and NlpE as sensors, respectively. In
this review, we focus on these two OM sensors. β-Barrel assembly machinery (BAM) inserts transmembrane OM proteins
(OMPs) into the OM. BAM co-assembles RcsF, the Rcs sensor, with OMPs, forming the RcsF-OMP complex. Researchers
have presented two models for stress sensing in the Rcs pathway. The first model suggests that LPS perturbation stress
disassembles the RcsF-OMP complex, freeing RcsF to activate Rcs. The second model proposes that BAM cannot assemble
RcsF into OMPs when the OM or PG is under specific stresses, and thus, the unassembled RcsF activates Rcs. These two
models may not be mutually exclusive. Here, we evaluate these two models critically in order to elucidate the stress sensing
mechanism. NlpE, the Cpx sensor, has an N-terminal (NTD) and a C-terminal domain (CTD). A defect in lipoprotein trafficking
results
in NlpE retention in the inner membrane, provoking the Cpx response. Signaling requires the NlpE NTD, but not the NlpE CTD; however, OM-anchored NlpE senses adherence to a hydrophobic surface, with the NlpE CTD playing a key role in this function. -
Citations
Citations to this article as recorded by
- Transcriptome reveals the role of the htpG gene in mediating antibiotic resistance through cell envelope modulation in Vibrio mimicus SCCF01
Zhenyang Qin, Kun Peng, Yang Feng, Yilin Wang, Bowen Huang, Ziqi Tian, Ping Ouyang, Xiaoli Huang, Defang Chen, Weimin Lai, Yi Geng
Frontiers in Microbiology.2024;[Epub] CrossRef - Rcs signal transduction system in Escherichia coli: Composition, related functions, regulatory mechanism, and applications
Zeyu Li, Yingying Zhu, Wenli Zhang, Wanmeng Mu
Microbiological Research.2024; 285: 127783. CrossRef - Identification of genes used by Escherichia coli to mitigate climatic stress conditions
Styliani Roufou, Sholeem Griffin, Lydia Katsini, Monika Polańska, Jan F.M. Van Impe, Panagiotis Alexiou, Vasilis P. Valdramidis
Gene Reports.2024; 36: 101998. CrossRef - The Role of Propionate-Induced Rearrangement of Membrane Proteins in the Formation of the Virulent Phenotype of Crohn’s Disease-Associated Adherent-Invasive Escherichia coli
Olga V. Pobeguts, Maria A. Galyamina, Elena V. Mikhalchik, Sergey I. Kovalchuk, Igor P. Smirnov, Alena V. Lee, Lyubov Yu. Filatova, Kirill V. Sikamov, Oleg M. Panasenko, Alexey Yu. Gorbachev
International Journal of Molecular Sciences.2024; 25(18): 10118. CrossRef - CpxAR two-component system contributes to virulence properties of Cronobacter sakazakii
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Food Microbiology.2024; 117: 104393. CrossRef - Breaking Barriers: Exploiting Envelope Biogenesis and Stress Responses to Develop Novel Antimicrobial Strategies in Gram-Negative Bacteria
Renu Bisht, Pierre D. Charlesworth, Paola Sperandeo, Alessandra Polissi
Pathogens.2024; 13(10): 889. CrossRef - The protective role of potassium in the adaptation of Pseudomonas protegens SN15-2 to hyperosmotic stress
Jian Wang, Yaping Wang, Shouquan Lu, Haibo Lou, XiaoBing Wang, Wei Wang
Microbiological Research.2024; 289: 127887. CrossRef - Bacterial Regulatory Mechanisms for the Control of Cellular Processes: Simple Organisms’ Complex Regulation
Jin-Won Lee
Journal of Microbiology.2023; 61(3): 273. CrossRef - Physiological and Transcriptomic Analyses of Escherichia coli Serotype O157:H7 in Response to Rhamnolipid Treatment
Shuo Yang, Lan Ma, Xiaoqing Xu, Qing Peng, Huiying Zhong, Yuxin Gong, Linbo Shi, Mengxin He, Bo Shi, Yu Qiao
Microorganisms.2023; 11(8): 2112. CrossRef
- Transcriptome reveals the role of the htpG gene in mediating antibiotic resistance through cell envelope modulation in Vibrio mimicus SCCF01
Journal Article
- Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate
- Woo-Chang Chung , Kwang Yeon Hwang , Suk-Jo Kang , Jae-Ouk Kim , Moon Jung Song
- J. Microbiol. 2020;58(1):46-53. Published online November 25, 2019
- DOI: https://doi.org/10.1007/s12275-020-9384-0
- 44 View
- 0 Download
- 7 Web of Science
- 5 Crossref
-
Abstract
- The Chikungunya virus (CHIKV) belongs to the Alphavirus genus of Togaviridae family and contains a positive-sense single stranded RNA genome. Infection by this virus mainly causes sudden high fever, rashes, headache, and severe joint pain that can last for several months or years. CHIKV, a mosquito- borne arbovirus, is considered a re-emerging pathogen that has become one of the most pressing global health concerns due to a rapid increase in epidemics. Because handling of CHIKV is restricted to Biosafety Level 3 (BSL-3) facilities, the evaluation of prophylactic vaccines or antivirals has been substantially hampered. In this study, we first identified the whole structural polyprotein sequence of a CHIKV strain isolated in South Korea (KNIH/2009/77). Phylogenetic analysis showed that this sequence clustered within the East/ Central/South African CHIKV genotype. Using this sequence information, we constructed a CHIKV-pseudotyped lentivirus expressing the structural polyprotein of the Korean CHIKV isolate (CHIKVpseudo) and dual reporter genes of green fluorescence protein and luciferase. We then developed a pseudovirus-based neutralization assay (PBNA) using CHIKVpseudo. Results from this assay compared to those from the conventional plaque reduction neutralization test showed that our PBNA was a reliable and rapid method to evaluate the efficacy of neutralizing antibodies. More importantly, the neutralizing activities of human sera from CHIKVinfected individuals were quantitated by PBNA using CHIKVpseudo. Taken together, these results suggest that our PBNA for CHIKV may serve as a useful and safe method for testing the neutralizing activity of antibodies against CHIKV in BSL-2 facilities.
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Citations
Citations to this article as recorded by- Identification of RACK1 as a novel regulator of non-structural protein 4 of chikungunya virus
Yao Yan, Fengyuan Zhang, Meng Zou, Hongyu Chen, Jingwen Xu, Shuaiyao Lu, Hongqi Liu
Acta Biochimica et Biophysica Sinica.2024; 56(10): 1425. CrossRef - Facile quantitative diagnostic testing for neutralizing antibodies against Chikungunya virus
Hui-Chung Lin, Shu-Fen Chang, Chien-Ling Su, Huai-Chin Hu, Der-Jiang Chiao, Yu-Lin Hsu, Hsuan-ying Lu, Chang-Chi Lin, Pei-Yun Shu, Szu-Cheng Kuo
BMC Infectious Diseases.2024;[Epub] CrossRef - Development of a Novel Chikungunya Virus-Like Replicon Particle for Rapid Quantification and Screening of Neutralizing Antibodies and Antivirals
Hui-Chung Lin, Der-Jiang Chiao, Pei-Yun Shu, Hui-Tsu Lin, Chia-Chu Hsiung, Chang-Chi Lin, Szu-Cheng Kuo, Juan E. Ludert
Microbiology Spectrum.2023;[Epub] CrossRef - Preparation and application of chikungunya pseudovirus containing double reporter genes
Chunyan Su, Kaiyun Ding, Jingwen Xu, Jianchao Wu, Jiansheng Liu, Jiayuan Shen, Hongning Zhou, Hongqi Liu
Scientific Reports.2022;[Epub] CrossRef - Prevalence of Malaria and Chikungunya Co-Infection in Febrile Patients: A Systematic Review and Meta-Analysis
Wanida Mala, Polrat Wilairatana, Kwuntida Uthaisar Kotepui, Manas Kotepui
Tropical Medicine and Infectious Disease.2021; 6(3): 119. CrossRef
- Identification of RACK1 as a novel regulator of non-structural protein 4 of chikungunya virus
Validation Study
- Comparison of JEV Neutralization Assay Using Pseudotyped JEV with the Conventional Plaque-Reduction Neutralization Test
- Hee-Jung Lee , Kyung-Il Min , Ki Hoon Park , Hyo Jung Choi , Min-Kyoung Kim , Chi-Young Ahn , Young-Jin Hong , Young Bong Kim
- J. Microbiol. 2014;52(5):435-440. Published online March 7, 2014
- DOI: https://doi.org/10.1007/s12275-014-3529-y
- 41 View
- 0 Download
- 12 Crossref
-
Abstract
- We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV- PV). JEV-PV-based neutralization assay offers several advan-tages compared with the current standard plaque-reduc-tion neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque re-duction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.
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Citations
Citations to this article as recorded by- Role of pseudotyped viruses in understanding epidemiology, pathogenesis and immunity of viral diseases affecting both horses and humans
Rebecca L. Sedgwick, Ola ElBohy, Janet M. Daly
Virology.2024; 597: 110164. CrossRef - Correlation between pseudotyped virus and authentic virus neutralisation assays, a systematic review and meta-analysis of the literature
Diego Cantoni, Craig Wilkie, Emma M. Bentley, Martin Mayora-Neto, Edward Wright, Simon Scott, Surajit Ray, Javier Castillo-Olivares, Jonathan Luke Heeney, Giada Mattiuzzo, Nigel James Temperton
Frontiers in Immunology.2023;[Epub] CrossRef - Changes in age-specific seroprevalence of Japanese encephalitis virus and impact of Japanese encephalitis vaccine in Korea
Byung Ok Kwak, Young Jin Hong, Dong Hyun Kim
Clinical and Experimental Pediatrics.2022; 65(3): 108. CrossRef - Prevalence of Malaria and Chikungunya Co-Infection in Febrile Patients: A Systematic Review and Meta-Analysis
Wanida Mala, Polrat Wilairatana, Kwuntida Uthaisar Kotepui, Manas Kotepui
Tropical Medicine and Infectious Disease.2021; 6(3): 119. CrossRef - Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate
Woo-Chang Chung, Kwang Yeon Hwang, Suk-Jo Kang, Jae-Ouk Kim, Moon Jung Song
Journal of Microbiology.2020; 58(1): 46. CrossRef - Retention of neutralizing antibodies to Japanese encephalitis vaccine in age groups above fifteen years in Korea
Hee-Jung Lee, Hanul Choi, Ki Hoon Park, Yuyeon Jang, Young-jin Hong, Young Bong Kim
International Journal of Infectious Diseases.2020; 100: 53. CrossRef - Graphene functionalized field-effect transistors for ultrasensitive detection of Japanese encephalitis and Avian influenza virus
Akanksha Roberts, Neha Chauhan, Saurav Islam, Subhasis Mahari, Bhaskar Ghawri, Ravi Kumar Gandham, S. S. Majumdar, Arindam Ghosh, Sonu Gandhi
Scientific Reports.2020;[Epub] CrossRef - Seroprevalence of Neutralizing Antibodies against Japanese Encephalitis Virus among Adolescents and Adults in Korea: A Prospective Multicenter Study
Byung Ok Kwak, Young Se Kwon, Young Jin Hong, Chung Hyun Nahm, Woori Jang, Young Uh, Yong Gon Cho, Jimyung Kim, Myungshin Kim, Dong Hyun Kim
Vaccines.2020; 8(2): 328. CrossRef - The Functions and Mechanism of a New Oligopeptide BP9 from Avian Bursa on Antibody Responses, Immature B Cell, and Autophagy
Xiu Li Feng, Man Man Zong, Guang Fang Zhou, Yang Zheng, Yuan Nan Yu, Rui Bing Cao, Pu Yan Chen, Mei Yang
Journal of Immunology Research.2019; 2019: 1. CrossRef - MLKL Mediated Necroptosis Accelerates JEV-Induced Neuroinflammation in Mice
Peiyu Bian, Xuyang Zheng, Li Wei, Chuantao Ye, Hong Fan, Yanhui Cai, Ying Zhang, Fanglin Zhang, Zhansheng Jia, Yingfeng Lei
Frontiers in Microbiology.2017;[Epub] CrossRef - Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus
Suk Fun Chin, Lih Shan Lim, Suh Cem Pang, Magdline Sia Henry Sum, David Perera
Microchimica Acta.2017; 184(2): 491. CrossRef - Mesenchymal stem cells alleviate Japanese encephalitis virus-induced neuroinflammation and mortality
Peiyu Bian, Chuantao Ye, Xuyang Zheng, Jing Yang, Wei Ye, Yuan Wang, Yun Zhou, Hongwei Ma, Peijun Han, Hai Zhang, Ying Zhang, Fanglin Zhang, Yingfeng Lei, Zhansheng Jia
Stem Cell Research & Therapy.2017;[Epub] CrossRef
- Role of pseudotyped viruses in understanding epidemiology, pathogenesis and immunity of viral diseases affecting both horses and humans
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