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- Descr!ption of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β‑cryptoxanthin Isolated from the Tongtian River Sediments
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Yuyuan Huang , Yifan Jiao , Sihui Zhang , Yuanmeihui Tao , Suping Zhang , Dong Jin , Ji Pu , Liyun Liu , Jing Yang , Shan Lu
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J. Microbiol. 2023;61(4):379-388. Published online March 16, 2023
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DOI: https://doi.org/10.1007/s12275-023-00029-5
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Abstract
- Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and
JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E)
in July 2019. Both strains were shown to grow at 15–35 °C and pH 7.0–10.0, and in the presence of 0–6.0% (w/v) NaCl.
The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH
30183
T (98.6–98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T
(98.5–98.6%) and O. murale 01-Gi-040T
(98.3–98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively,
revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization
(dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other
Ornithinimicrobium species were within the ranges of 19.0–23.9% and 70.8–80.4%, respectively, all below the respective
recommended 70.0% and 95–96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and
JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant
menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin (
C40H56O) can
be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic,
and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which
the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
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