Journal Article
- Host-specificity of symbiotic mycorrhizal fungi for enhancing seed germination, protocorm formation and seedling development of over-collected medicinal orchid, Dendrobium devonianum
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Hui Huang , Xiao-Meng Zi , Hua Lin , Jiang-Yun Gao
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J. Microbiol. 2018;56(1):42-48. Published online January 4, 2018
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DOI: https://doi.org/10.1007/s12275-018-7225-1
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Abstract
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All orchids maintain an obligate relationship with mycorrhizal
symbionts during seed germination. In most cases,
germination-enhancing fungi have been isolated from roots
of mature plants for conservation and cultivation purposes.
To understand the germination biology of Dendrobium devonianum,
an over-collected medicinal orchid, the seeds of
D. devonianum were inoculated with a fungal strain (FDd1)
isolated from naturally occurring protocorms of D. devonianum
and two other germination-enhancing fungal strains
(FDaI7 and FCb4) from D. aphyllum and Cymbidium mannii,
respectively. The fungal strain was isolated from five
protocorms of D. devonianum and identified as a species of
the genus Epulorhiza. In germination trials, treatments with
all of the three fungal strains showed a significant promoting
effect on seed germination and protocorm formation, compared
with the control treatment (no inoculation). However,
FDd1 fungal strain showed the greatest effectiveness followed
by FDaI7 and FCb4. For all inoculation and control treatments,
seeds developed to protocorms regardless of the presence
of illumination, whereas protocorms did not develop
to seedlings unless illumination was provided. The results
of our manipulative experiments confirmed the hypothesis
that mycorrhizae associated with orchid seedlings are highly
host-specific, and the degree of specificity may be life stagespecific
under in vitro conditions. The specific mycorrhizal
symbionts from protocorms can enhance restoration efforts
and the conservation of orchids such as D. devonianum.
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Research Support, Non-U.S. Gov't
- Arbuscular mycorrhizal fungal diversity, root colonization, and soil alkaline phosphatase activity in response to maize-wheat rotation and no-tillage in North China
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Junli Hu , Anna Yang , Anning Zhu , Junhua Wang , Jue Dai , Ming Hung Wong , Xiangui Lin
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J. Microbiol. 2015;53(7):454-461. Published online June 27, 2015
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DOI: https://doi.org/10.1007/s12275-015-5108-2
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Abstract
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Monitoring the effects of no-tillage (NT) in comparison with
conventional tillage (CT) on soil microbes could improve
our understanding of soil biochemical processes and thus
help us to develop sound management strategies. The objective
of this study was to compare the species composition and
ecological function of soil arbuscular mycorrhizal (AM) fungi
during the growth and rotation of crops under NT and CT.
From late June 2009 to early June 2010, 32 topsoil (0쭯15 cm)
samples from four individual plots per treatment (CT and
NT) were collected at both the jointing and maturation stages
of maize (Zea mays L.) and wheat (Triticum aestivum L.)
from a long-term experimental field that was established in
an Aquic Inceptisol in North China in June 2006. The AM
fungal spores were isolated and identified and then used to
calculate species diversity indices, including the Shannon-
Wiener index (H▽), Evenness (E), and Simpson’s index (D).
The root mycorrhizal colonization and soil alkaline phosphatase
activity were also determined. A total of 34 species
of AM fungi within nine genera were recorded. Compared
with NT, CT negatively affected the soil AM fungal community
at the maize sowing stage, leading to decreases in the
average diversity indices (from 2.12, 0.79, and 0.82 to 1.79,
0.72, and 0.74 for H▽, E, and D, respectively), root mycorrhizal
colonization (from 28% to 20%), soil alkaline phosphatase
activity (from 0.24 to 0.19 mg/g/24 h) and available phosphorus concentration (from 17.4 to 10.5 mg/kg) at the
maize jointing stage. However, reductions in diversity indices
of H▽, E, and D were restored to 2.20, 0.81, and 0.84,
respectively, at the maize maturation stage. CT should affect
the community again at the wheat sowing stage; however, a
similar restoration in the species diversity of AM fungi was
completed before the wheat jointing stage, and the highest
Jaccard index (0.800) for similarity in the species composition
of soil AM fungi between CT and NT was recorded at
the wheat maturation stage. Our results also demonstrated
that NT resulted in the positive protection of the community
structure of AM fungi and played an important role in maintaining
their functionality especially for maize seedlings.
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