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- Development of recombinant Yarrowia lipolytica producing virus-like particles of a fish nervous necrosis virus
- Van-Trinh Luu , Hye Yun Moon , Jee Youn Hwang , Bo-Kyu Kang , Hyun Ah Kang
- J. Microbiol. 2017;55(8):655-664. Published online July 28, 2017
- DOI: https://doi.org/10.1007/s12275-017-7218-5
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- 19 Crossref
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Abstract
- Nervous necrosis virus (NNV) causes viral encephalopathy and retinopathy, a devastating disease of many species of cultured marine fish worldwide. In this study, we used the dimorphic non-pathogenic yeast Yarrowia lipolytica as a host to express the capsid protein of red-spotted grouper nervous necrosis virus (RGNNV-CP) and evaluated its potential as a platform for vaccine production. An initial attempt was made to express the codon-optimized synthetic genes encoding intact and N-terminal truncated forms of RGNNV-CP under the strong constitutive TEF1 promoter using autonomously replicating sequence (ARS)-based vectors. The full-length recombinant capsid proteins expressed in Y. lipolytica were detected not only as monomers and but also as trimers, which is a basic unit for formation of NNV virus-like particles (VLPs). Oral immunization of mice with whole recombinant Y. lipolytica harboring the ARSbased plasmids was shown to efficiently induce the formation of IgG against RGNNV-CP. To increase the number of integrated copies of the RGNNV-CP expression cassette, a set of 26S ribosomal DNA-based multiple integrative vectors was constructed in combination with a series of defective Ylura3 with truncated promoters as selection markers, resulting in integrants harboring up to eight copies of the RGNNVCP cassette. Sucrose gradient centrifugation and transmission electron microscopy of this high-copy integrant were carried out to confirm the expression of RGNNV-CPs as VLPs. This is the first report on efficient expression of viral capsid proteins as VLPs in Y. lipolytica, demonstrating high potential for the Y. lipolytica expression system as a platform for recombinant vaccine production based on VLPs.
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Citations
Citations to this article as recorded by
- Elucidation and engineering of Sphingolipid biosynthesis pathway in Yarrowia lipolytica for enhanced production of human-type sphingoid bases and glucosylceramides
Seo Hyeon Shin, Hye Yun Moon, Hae Eun Park, Gi Jeong Nam, Ju Hye Baek, Che Ok Jeon, Hyunwook Jung, Myeong Seok Cha, Sol Choi, Jeong Jun Han, Chen Yuan Hou, Chang Seo Park, Hyun Ah Kang
Metabolic Engineering.2025; 87: 68. CrossRef - Yeast-Based Virus-like Particles as an Emerging Platform for Vaccine Development and Delivery
Vartika Srivastava, Kripa N. Nand, Aijaz Ahmad, Ravinder Kumar
Vaccines.2023; 11(2): 479. CrossRef - Humoral immune response in Asian seabass vaccinated with inactivated and recombinant viral nervous necrosis vaccine
M. Makesh, N. Venkata Satyanarayana, K. Muddukrishnaiah, Sujeet Kumar, G. Thiagarajan, Ashok Kumar Jangam, R. Subburaj, M. Kailasam, K.K. Vijayan
Aquaculture.2023; 569: 739384. CrossRef - Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts
Xiaosong Gu, Lei Huang, Jiazhang Lian
Synthetic and Systems Biotechnology.2023; 8(3): 469. CrossRef - Yeast as carrier for drug delivery and vaccine construction
Yifu Tan, Liwei Chen, Ke Li, Beibei Lou, Yanfei Liu, Zhenbao Liu
Journal of Controlled Release.2022; 346: 358. CrossRef - Construction of Attenuated Strains for Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) via Reverse Genetic System
Yingying Lei, Yu Xiong, Dagang Tao, Tao Wang, Tianlun Chen, Xufei Du, Gang Cao, Jiagang Tu, Jinxia Dai
Viruses.2022; 14(8): 1737. CrossRef - Long-Term Protection and Serologic Response of European Sea Bass Vaccinated with a Betanodavirus Virus-Like Particle Produced in Pichia pastoris
Sofie Barsøe, Anna Toffan, Francesco Pascoli, Ansgar Stratmann, Tobia Pretto, Andrea Marsella, Mériem Er-Rafik, Niccolò Vendramin, Niels J. Olesen, Dagoberto Sepúlveda, Niels Lorenzen
Vaccines.2021; 9(5): 447. CrossRef - Plant-Produced Vaccines: Future Applications in Aquaculture
Hang Su, Igor A. Yakovlev, André van Eerde, Jianguo Su, Jihong Liu Clarke
Frontiers in Plant Science.2021;[Epub] CrossRef - Recombinant Baculovirus-Produced Grass Carp Reovirus Virus-Like Particles as Vaccine Candidate That Provides Protective Immunity against GCRV Genotype II Infection in Grass Carp
Ting Gao, Caixia Gao, Siyu Wu, Yingying Wang, Jiyuan Yin, Yingying Li, Weiwei Zeng, Sven M. Bergmann, Qing Wang
Vaccines.2021; 9(1): 53. CrossRef - Developing oral nanovaccines for fish: a modern trend to fight infectious diseases
Carlos Angulo, Marlene Tello‐Olea, Martha Reyes‐Becerril, Elizabeth Monreal‐Escalante, Luis Hernández‐Adame, Miriam Angulo, José M. Mazon‐Suastegui
Reviews in Aquaculture.2021; 13(3): 1172. CrossRef - Contribution of yeast models to virus research
R Sahaya Glingston, Jyoti Yadav, Jitika Rajpoot, Neha Joshi, Shirisha Nagotu
Applied Microbiology and Biotechnology.2021; 105(12): 4855. CrossRef - Yarrowia lipolytica, health benefits for animals
Francisco A. Guardiola, María Ángeles Esteban, Carlos Angulo
Applied Microbiology and Biotechnology.2021; 105(20): 7577. CrossRef - Betanodavirus and VER Disease: A 30-year Research Review
Isabel Bandín, Sandra Souto
Pathogens.2020; 9(2): 106. CrossRef - Yeast synthetic biology for designed cell factories producing secretory recombinant proteins
Eun Jung Thak, Su Jin Yoo, Hye Yun Moon, Hyun Ah Kang
FEMS Yeast Research.2020;[Epub] CrossRef - Yeast-based vaccines: New perspective in vaccine development and application
Ravinder Kumar, Piyush Kumar
FEMS Yeast Research.2019;[Epub] CrossRef - Development of conditional cell lysis mutants of Saccharomyces cerevisiae as production hosts by modulating OCH1 and CHS3 expression
Van-Trinh Luu, Hye Yun Moon, Su Jin Yoo, Jin Ho Choo, Eun Jung Thak, Hyun Ah Kang
Applied Microbiology and Biotechnology.2019; 103(5): 2277. CrossRef - An effective and rapid method for RNA preparation from non-conventional yeast species
Dong Wook Lee, Chang Pyo Hong, Hyun Ah Kang
Analytical Biochemistry.2019; 586: 113408. CrossRef - A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches
Jie Ma, Timothy J. Bruce, Evan M. Jones, Kenneth D. Cain
Microorganisms.2019; 7(11): 569. CrossRef - Vaccination with UV-inactivated nodavirus partly protects European sea bass against infection, while inducing few changes in immunity
Yulema Valero, Djamal Mokrani, Elena Chaves-Pozo, Marta Arizcun, Mustapha Oumouna, José Meseguer, M.Ángeles Esteban, Alberto Cuesta
Developmental & Comparative Immunology.2018; 86: 171. CrossRef
- Elucidation and engineering of Sphingolipid biosynthesis pathway in Yarrowia lipolytica for enhanced production of human-type sphingoid bases and glucosylceramides
Research Support, Non-U.S. Gov't
- Molecular Characterization of Polychlorinated Biphenyl-Dechlorinating Populations in Contaminated Sediments
- Kyoung-Hee Oh , Ellen B. Ostrofsky , Young-Cheol Cho
- J. Microbiol. 2008;46(2):165-173. Published online June 11, 2008
- DOI: https://doi.org/10.1007/s12275-007-0214-4
- 35 View
- 0 Download
- 11 Scopus
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Abstract
- Polychlorinated biphenyl (PCB)-dechlorinating microorganisms were characterized in PCB-contaminated sediments using amplified ribosomal DNA restriction analysis (ARDRA). The sediments were prepared by spiking Aroclor 1248 into PCB-free sediments, and were inoculated with microorganisms eluted from St. Lawrence River sediments. PCB-free sediments inoculated with the same inoculum served as the control. Four restriction fragment length polymorphism (RFLP) groups in the eubacterial and two in the archaeal domain were found exclusively in PCB-spiked sediment clone libraries. Sequence analysis of the four eubacterial clones showed homology to Escherichia coli, Lactosphaera pasteurii, Clostridium thermocellum, and Dehalobacter restrictus. The predominant archaeal sequence in the PCB-spiked sediment clone library was closely related to Methanosarcina barkeri, which appear to support earlier findings that methanogens are involved in PCB dechlorination. When the dot-blot hybridization was performed between the sediment DNA extract and the probes designed with eubacterial RFLP groups, the intensity of two of eubacterial RFLP groups, which showed high sequence homology to C. pascui and D. restrictus, was highly correlated with the number of dechlorinating microorganisms suggesting these two members intend to contribute to PCB dechlorination.
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