MSK
Search
- Page Path
- HOME > Search
Journal Articles
- Probiotic supplements alleviate gestational diabetes mellitus by restoring the diversity of gut microbiota: a study based on 16S rRNA sequencing
- Qing-Xiang Zheng , Xiu-Min Jiang , Hai-Wei Wang , Li Ge , Yu-Ting Lai , Xin-Yong Jiang , Fan Chen , Ping-Ping Huang
- J. Microbiol. 2021;59(9):827-839. Published online August 12, 2021
- DOI: https://doi.org/10.1007/s12275-021-1094-8
- 62 View
- 0 Download
- 15 Web of Science
- 15 Crossref
-
Abstract
- Probiotics effectively prevent and improve metabolic diseases such as diabetes by regulating the intestinal microenvironment and gut microbiota. However, the effects of probiotics in gestational diabetes mellitus are not clear. Here, we showed that probiotic supplements significantly improved fasting blood glucose in a gestational diabetes mellitus rat model. To further understand the mechanisms of probiotics in gestational diabetes mellitus, the gut microbiota were analyzed via 16S rRNA sequencing. We found that compared with the normal pregnant group, the gestational diabetes mellitus rats had decreased diversity of gut microbiota. Moreover, probiotic supplementation restored the diversity of the gut microbiota in gestational diabetes mellitus rats, and the gut microbiota structure tended to be similar to that of normal pregnant rats. In particular, compared with gestational diabetes mellitus rats, the abundance of Firmicutes and Actinobacteria was higher after probiotic supplementation. Furthermore, activating carbohydrate metabolism and membrane transport pathways may be involved in the potential mechanisms by which probiotic supplements alleviate gestational diabetes mellitus. Overall, our results suggested that probiotic supplementation might be a novel approach to restore the gut microbiota of gestational diabetes mellitus rats and provided an experimental evidence for the use of probiotic supplements to treat gestational diabetes melitus.
-
Citations
Citations to this article as recorded by
- Dietary Polyphenols Support Akkermansia muciniphila Growth via Mediation of the Gastrointestinal Redox Environment
Charlene B. Van Buiten, Valerie A. Seitz, Jessica L. Metcalf, Ilya Raskin
Antioxidants.2024; 13(3): 304. CrossRef - The Intervention of Probiotics on Type 2 Diabetes Mellitus in Animal Models
Qianyu Qu, Penggang He, Yuqi Zhang, Shujuan Yang, Peibin Zeng
Molecular Nutrition & Food Research.2024;[Epub] CrossRef - Lactobacillus gasseri BNR17 and Limosilactobacillus fermentum ABF21069 Ameliorate High Sucrose-Induced Obesity and Fatty Liver via Exopolysaccharide Production and β-oxidation
Yu Mi Jo, Yoon Ji Son, Seul-Ah Kim, Gyu Min Lee, Chang Won Ahn, Han-Oh Park, Ji-Hyun Yun
Journal of Microbiology.2024; 62(10): 907. CrossRef - Probiotic therapy as a promising strategy for gestational diabetes mellitus management
Deborah Emanuelle de Albuquerque Lemos, José Luiz de Brito Alves, Evandro Leite de Souza
Expert Opinion on Biological Therapy.2024; 24(11): 1207. CrossRef - Influence of Symbiotic Fermentation Broth on Regulating Metabolism with Gut Microbiota and Metabolite Profiles Is Estimated Using a Third-Generation Sequencing Platform
Chih-Yin Wu, Chun-Kai Huang, Wei-Sheng Hong, Yin-Hsiu Liu, Ming-Chi Shih, Jung-Chun Lin
Metabolites.2023; 13(9): 999. CrossRef - Neuroprotective Effect of Ponicidin Alleviating the Diabetic Cognitive Impairment: Regulation of Gut Microbiota
Xiaojuan Zhang, Feng Guo, Dujuan Cao, Yinan Yan, Ning Zhang, Kaili Zhang, Xinyi Li, Prashant Kumar, Xiaojuan Zhang
Applied Biochemistry and Biotechnology.2023; 195(2): 735. CrossRef - Antidiabetogenic mechanisms of probiotic action in food matrices: A review
Vanessa Moraes Ramalho Castro, Rosa Helena Luchese
PharmaNutrition.2022; 21: 100302. CrossRef - Prepregnancy body mass index and gestational weight gain are associated with maternal and infant adverse outcomes in Chinese women with gestational diabetes
Qing-Xiang Zheng, Hai-Wei Wang, Xiu-Min Jiang, Yan Lin, Gui-Hua Liu, Mian Pan, Li Ge, Xiao-Qian Chen, Jing-Ling Wu, Xiao-Yun Zhang, Yu-Qing Pan, Hong-Gu He
Scientific Reports.2022;[Epub] CrossRef - Probiotic Intervention in the Treatment of Diabetes Mellitus: A Review
Navya Sreepathi, M.K. Jayanthi, S. Jagadeep Chandra, Shrisha Naik Bajpe, Ramith Ramu
Journal of Pure and Applied Microbiology.2022; 16(3): 1519. CrossRef - Ameliorative Effects of Bifidobacterium animalis subsp. lactis J-12 on Hyperglycemia in Pregnancy and Pregnancy Outcomes in a High-Fat-Diet/Streptozotocin-Induced Rat Model
Jianjun Yang, Yumeng Ma, Tong Li, Yuanxiang Pang, Hongxing Zhang, Yuanhong Xie, Hui Liu, Yanfang Sun, Jianhua Ren, Junhua Jin
Nutrients.2022; 15(1): 170. CrossRef - Probiotic Mechanisms Affecting Glucose Homeostasis: A Scoping Review
Maša Pintarič, Tomaž Langerholc
Life.2022; 12(8): 1187. CrossRef - The Roles of Probiotics in the Gut Microbiota Composition and Metabolic Outcomes in Asymptomatic Post-Gestational Diabetes Women: A Randomized Controlled Trial
Zubaidah Hasain, Raja Affendi Raja Ali, Hajar Fauzan Ahmad, Ummul Fahri Abdul Rauf, Seok Fang Oon, Norfilza Mohd Mokhtar
Nutrients.2022; 14(18): 3878. CrossRef - Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation
Qing-Xiang Zheng, Hai-Wei Wang, Xiu-Min Jiang, Li Ge, Yu-Ting Lai, Xin-Yong Jiang, Ping-Ping Huang, Fan Chen, Xiao-Qian Chen
Frontiers in Microbiology.2022;[Epub] CrossRef - Microorganisms in the reproductive system and probiotic's regulatory effects on reproductive health
Tao Feng, Yan Liu
Computational and Structural Biotechnology Journal.2022; 20: 1541. CrossRef - Several Shaping Characteristics of OneCurve Continuously Rotating System versus Three Different Kinematic Systems: ProTaper Universal, Twisted File Adaptive and WaveOne Gold
Ali Türkyılmaz, Volkan Arıkan
Meandros Medical and Dental Journal.2022; 23(1): 67. CrossRef
- Dietary Polyphenols Support Akkermansia muciniphila Growth via Mediation of the Gastrointestinal Redox Environment
- STATR: A simple analysis pipeline of Ribo-Seq in bacteria
- Donghui Choe , Bernhard Palsson , Byung-Kwan Cho
- J. Microbiol. 2020;58(3):217-226. Published online January 28, 2020
- DOI: https://doi.org/10.1007/s12275-020-9536-2
- 49 View
- 0 Download
- 7 Web of Science
- 8 Crossref
-
Abstract
- Gene expression changes in response to diverse environmental stimuli to regulate numerous cellular functions. Genes are expressed into their functional products with the help of messenger RNA (mRNA). Thus, measuring levels of mRNA in cells is important to understand cellular functions. With advances in next-generation sequencing (NGS), the abundance of cellular mRNA has been elucidated via transcriptome sequencing. However, several studies have found a discrepancy between mRNA abundance and protein levels induced by translational regulation, including different rates of ribosome entry and translational pausing. As such, the levels of mRNA are not necessarily a direct representation of the protein levels found in a cell. To determine a more precise way to measure protein expression in cells, the analysis of the levels of mRNA associated with ribosomes is being adopted. With an aid of NGS techniques, a single nucleotide resolution footprint of the ribosome was determined using a method known as Ribo- Seq or ribosome profiling. This method allows for the highthroughput measurement of translation in vivo, which was further analyzed to determine the protein synthesis rate, translational pausing, and cellular responses toward a variety of environmental changes. Here, we describe a simple analysis pipeline for Ribo-Seq in bacteria, so-called simple translatome analysis tool for Ribo-Seq (STATR). STATR can be used to carry out the primary processing of Ribo-Seq data, subsequently allowing for multiple levels of translatome study, from experimental validation to in-depth analyses. A command- by-command explanation is provided here to allow a broad spectrum of biologists to easily reproduce the analysis.
-
Citations
Citations to this article as recorded by- Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation
Olga Iwańska, Przemysław Latoch, Natalia Kopik, Mariia Kovalenko, Małgorzata Lichocka, Remigiusz Serwa, Agata L. Starosta
Nature Communications.2024;[Epub] CrossRef - Comparative Transcriptome Analysis of Zerumbone-Treated Helicobacter pylori
Hyun Jun Woo, Ji Yeong Yang, Sa-Hyun Kim
Microbiology and Biotechnology Letters.2022; 50(2): 301. CrossRef - Synthetic 3′-UTR valves for optimal metabolic flux control in Escherichia coli
Donghui Choe, Kangsan Kim, Minjeong Kang, Seung-Goo Lee, Suhyung Cho, Bernhard Palsson, Byung-Kwan Cho
Nucleic Acids Research.2022; 50(7): 4171. CrossRef - Comparative Transcriptome Analysis of Caryophyllene- Treated Helicobacter pylori
Hyun Jun Woo, Ji Yeong Yang, Hye Jin Kwon, Hyun Woo Kim, Sa-Hyun Kim, Jong-Bae Kim
Microbiology and Biotechnology Letters.2021;[Epub] CrossRef - RiboRid: A low cost, advanced, and ultra-efficient method to remove ribosomal RNA for bacterial transcriptomics
Donghui Choe, Richard Szubin, Saugat Poudel, Anand Sastry, Yoseb Song, Yongjae Lee, Suhyung Cho, Bernhard Palsson, Byung-Kwan Cho, Lefu Lan
PLOS Genetics.2021; 17(9): e1009821. CrossRef - Omics-based microbiome analysis in microbial ecology: from sequences to information
Jang-Cheon Cho
Journal of Microbiology.2021; 59(3): 229. CrossRef - HRIBO: high-throughput analysis of bacterial ribosome profiling data
Rick Gelhausen, Sarah L Svensson, Kathrin Froschauer, Florian Heyl, Lydia Hadjeras, Cynthia M Sharma, Florian Eggenhofer, Rolf Backofen, Valencia Alfonso
Bioinformatics.2021; 37(14): 2061. CrossRef - User guides for biologists to learn computational methods
Dokyun Na
Journal of Microbiology.2020; 58(3): 173. CrossRef
- Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation
First
Prev
TOP
