Journal Article
- A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium
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Jin-Il Park , Sun-Min Seo , Jong-Hyung Park , Hee-Yeon Jeon , Jun-Young Kim , Seung-Hyun Ryu , Yang-Kyu Choi
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J. Microbiol. 2018;56(4):272-279. Published online April 2, 2018
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DOI: https://doi.org/10.1007/s12275-018-7504-x
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Abstract
- Adult mice were treated with dextran sulfate sodium (DSS)
and infected with Citrobacter rodentium for developing a
novel murine colitis model. C57BL/6N mice (7-week-old)
were divided into four groups. Each group composed of control,
dextran sodium sulfate-treated (DSS), C. rodentiuminfected
(CT), and DSS-treated and C. rodentium-infected
(DSS-CT) mice. The DSS group was administered 1% DSS
in drinking water for 7 days. The CT group was supplied
with normal drinking water for 7 days and subsequently infected
with C. rodentium via oral gavage. The DSS-CT group
was supplied with 1% DSS in drinking water for 7 days and
subsequently infected with C. rodentium via oral gavage. The
mice were sacrificed 10 days after the induction of C. rodentium
infection. The DSS-CT group displayed significantly
shorter colon length, higher spleen to body weight ratio, and
higher histopathological score compared to the other three
groups. The mRNA expression levels of tumor necrosis factor
(TNF)-α and interferon (INF)-γ were significantly upregulated;
however, those of interleukin (IL)-6 and IL-10 were
significantly downregulated in the DSS-CT group than in
the control group. These results demonstrated that a combination
of low DSS concentration (1%) and C. rodentium
infection could effectively induce inflammatory bowel disease
(IBD) in mice. This may potentially be used as a novel
IBD model, in which colitis is induced in mice by the combination
of a chemical and a pathogen.
Research Support, Non-U.S. Gov't
- NOTE] The Presence of Borrelia valaisiana-Related Genospecies in Ticks and a Rodent in Taiwan
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Chun-Man Huang , Hsi-Chieh Wang , Ying-Chun Lin , Shih-Hui Chiu , Ying-Shun Kao , Pei-Lung Lee , Hsiu-I Wang , Ruei-Chen Hung , Huang-I Chan , Ho-Sheng Wu , Chuen-Sheue Chiang , Jung-Jung Mu
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J. Microbiol. 2010;48(6):877-880. Published online January 9, 2011
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DOI: https://doi.org/10.1007/s12275-010-0331-3
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Abstract
- A field survey was conducted to investigate the presence of Borrelia burgdorferi sensu lato (s.l.) in six counties of Taiwan. Spirochetes were successfully isolated from one rodent ear sample out of 485 rodent ears and 53 live, fed tick (Ixodes granulatus) samples. The spirochetes were confirmed to be B. burgdorferi s.l. by real-time PCR. In addition, 23 of 113 tick samples were tested positive for Borrelia DNA according to real-time PCR. The Borrelia isolate from the rodent and the 23 Borrelia DNA samples from the ticks were identified as B. valaisiana-related genospecies by phylogenetic analysis based on flagellin gene sequences. These findings suggest that the Borrelia valaisiana-related strains are maintained in a zoonotic cycle between tick vectors and reservoir hosts in Taiwan.