Journal Article
- Rotavirus-mediated alteration of gut microbiota and its correlation with physiological characteristics in neonatal calves
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Ja-Young Jang , Suhee Kim , Min-Sung Kwon , Jieun Lee , Do-Hyeon Yu , Ru-Hui Song , Hak-Jong Choi , Jinho Park
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J. Microbiol. 2019;57(2):113-121. Published online November 19, 2018
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DOI: https://doi.org/10.1007/s12275-019-8549-1
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Abstract
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Diarrhea is a fatal disease to neonatal calves, and rotavirus is
the main pathogen associated with neonatal calf diarrhea.
Although previous studies have reported that the gut microbiota
is changed in calves during diarrhea, less is known
about whether rotavirus infection alters the structure of the
gut microbiota. Here, we characterized fecal microbial communities
and identified possible relationships between the
gut microbiota profiles and physiological parameters. Five
fecal specimens of rotavirus-infected calves from 1 to 30 days
after birth and five fecal specimens of age-matched healthy
calves were used for the microbial community analysis using
the Illumina MiSeq sequencer. Rotavirus infection was
associated with reduced rotavirus infection significantly reduced
the richness and diversity of the bacterial community.
Weighted unique fraction metric analysis exhibited significant
differences in community membership and structure between
healthy and rotavirus-infected calves. Based on relative abundance
analysis and linear discriminant analysis effect size, we
found that the representative genera from Lactobacillus, Subdoligranulum,
Blautia, and Bacteroides were closely related to
healthy calves, while the genera Escherichia and Clostridium
were closely affiliated to rotavirus-infected calves. Furthermore,
canonical correlation analysis and Pearson correlation
coefficient results revealed that the increased relative abundances
of Lactobacillus, Subdoligranulum, and Bacteroides
were correlated with normal levels of physiological characteristics
such as white blood cells, blood urea nitrogen, serum
amyloid protein A, and glucose concentration in serum. These
results
suggest that rotavirus infection alters the structure
of the gut microbiota, correlating changes in physiological
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Citations
Citations to this article as recorded by

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Shengwei Cui, Shihui Guo, Qingmei Zhao, Yong Li, Yun Ma, Yongtao Yu
Frontiers in Microbiology.2023;[Epub] CrossRef - Neonatal Calf Diarrhea Is Associated with Decreased Bacterial Diversity and Altered Gut Microbiome Profiles
Wei Li, Xin Yi, Baoyun Wu, Xiang Li, Boping Ye, Ziqi Deng, Runa A, Sanlong Hu, Dongdong Li, Hao Wu, Zhenming Zhou
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Journal of Animal Science and Biotechnology.2023;[Epub] CrossRef -
Intestinal mucin-type
O
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Naukovij vìsnik veterinarnoï medicini.2023; (2 (184)): 192. CrossRef - Analysis of Fecal Microbial Changes in Young Calves Following Bovine Rotavirus Infection
Seon-Ho Kim, Youyoung Choi, Michelle A. Miguel, Shin-Ja Lee, Sung-Sill Lee, Sang-Suk Lee
Veterinary Sciences.2023; 10(8): 496. CrossRef - Host innate immune responses and microbiome profile of neonatal calves challenged with Cryptosporidium parvum and the effect of bovine colostrum supplementation
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Weiwei Ni, Xinwei Jiao, Huihuang Zou, Mengjuan Jing, Ming Xia, Shichao Zhu, Liming Li
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Carolyn Bomidi, Matthew Robertson, Cristian Coarfa, Mary K. Estes, Sarah E. Blutt
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Xiang-Dong Zeng, Wen-Guang Hu
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Yanyan Wu, Lili Wang, Ruiqing Luo, Hongli Chen, Cunxi Nie, Junli Niu, Cheng Chen, Yongping Xu, Xiaoyu Li, Wenjun Zhang
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Mengling Yang, Yang Yang, Qingnan He, Ping Zhu, Mengqi Liu, Jiahao Xu, Mingyi Zhao
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Research Support, Non-U.S. Gov'ts
- Antiviral effects of Lactobacillus ruminis SPM0211 and Bifidobacterium longum SPM1205 and SPM1206 on rotavirus-infected Caco-2 cells and a neonatal mouse model
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Joo Yeon Kang , Do Kyung Lee , Nam Joo Ha , Hea Soon Shin
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J. Microbiol. 2015;53(11):796-803. Published online October 28, 2015
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DOI: https://doi.org/10.1007/s12275-015-5302-2
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Abstract
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Rotavirus is worldwide cause of severe gastroenteritis including
severe diarrhea and fatal dehydration in infants
and young children. There is an available vaccination program
for preventing rotavirus infection, but it has limits
and restrictions. Probiotics therapy could be an alternative
method
of antiviral prevention and modulation against rotavirus
infection. In this study, we screened the antiviral activity
of probiotic bacteria such as 3 Lactobacillus spp. and
14 Bifidobacterium spp. isolated from young Korean. Three
of the bacteria, Lactobacillus ruminis SPM0211, Bifidobacterium
longum SPM1205, and SPM1206, inhibited human
strain Wa rotavirus infection in Caco-2 cells. Furthermore,
these bacterial strains inhibited rotavirus replication in a
rotavirus-infected neonatal mouse model. To clarify the mechanism
of inhibition, we investigated gene expression of
Interferon (IFN)-signaling components and IFN-inducible
antiviral effectors. All 3 probiotics increased IFN-α and IFN-
β levels compared with the control. Gene expression of IFNsignaling
components and IFN-inducible antiviral effectors
also increased. Overall, these results indicate that L. ruminis
SPM0211, B. longum SPM1205 and 1206 efficiently inhibit
rotavirus replication in vitro and in vivo. Especially, the antiviral
effect of Lactobacillus ruminis SPM0211 is worthy of
notice. This is the first report of L. ruminis with antiviral activity.
Anti-rotaviral effects of the 3 probiotics are likely due
to their modulation of the immune response through promoting
type I IFNs, which are key regulators in IFN signaling
pathway.
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- NOTE] Molecular Characterization of Two Strains of Porcine Group C Rotavirus
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Sung-Geun Lee , Soo-Hyun Youn , Mi-Hwa Oh , Ok-Jae Rhee , Sangsuk Oh , Soon-Young Paik
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J. Microbiol. 2011;49(6):1058-1062. Published online December 28, 2011
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DOI: https://doi.org/10.1007/s12275-011-1088-z
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Abstract
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Group C rotaviruses are an important cause of acute gastroenteritis in humans and animals. Fecal samples were collected from a porcine herd in July, 2009. Group C rotavirus RNA was detected using RT-PCR for the VP6 gene. The identified strain was further characterized by sequencing and phylogenetic analysis of the partial VP4, and complete VP6 and VP7 gene sequences. The partial VP4 and complete VP6 gene sequences of the CUK-5 strain were most closely related to those of the CUK-6 strain of group C rotaviruses. Phylogenetic analysis of the VP7 gene of the 2 strains (CUK-5 and CUK-6) and reference strains of group G rotavirus by the neighbor-joining method also confirmed that CUK-5 and CUK-6 belonged to type G5 and G1 strains, respectively. This study provides useful data for the prediction of newly appearing variants of porcine group C rotaviruses in neighboring countries through comparisons with GCRVs and fundamental research for vaccine development.
- cDNA cloning and expresion of human rotavirus outer capsid protein VP4
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Kang, Seok W. , Yang, Jai M.
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J. Microbiol. 1998;36(3):214-221.
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Abstract
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cDNA for the VP4-coding RNA segment 4 of human rotavirus isolated from Korean patients Was synthesized and cloned (HRV-k41), and its nucleotide sequence was determined. Comparative analysis of the nucleotide sequence of JRV-k41 showed 90.6%, 86.6%, 74.6%, 66%, 70.1% and 65.4% homology to P1A[8](Wa), P1B[4](RV5), P2[6](1076), P3[9](AU1), P4[10](69M), and P[14](PA169) genotypes respectively. The deduced amino acid sequence homology of HRV-k41 to P1A[8](Wa), P1B[4](RV5), P2[6](1076), P3[9](AU1), P4[10](69M), and P[14](PA169) genotypes respectively. The deduced amino acid sequence homology of HRV-k41 to P1A[8](Wa), P1B[4](RV5), P2[6](1076), P3[9](AU1), P4[10](69M) and P[14](169) was 92.9% 89.7%. 75.8%, 64.1%, 70.6% and 64.3% respectively. These results suggest that HRV-k41 is closely related to the P1A genotype. Two trypsin cleavage sites (arginine 240 and arginine 246) and four cysteine residues (215, 317, 379, and 773) conserved in VP4 of all rotavirus strains were also found in JRV-k41. Similar to other virulent human rotaviruses, an additional trypsin cleavage site(lysine 245) was also detected in this strain. The cDNA of the VP4-coding RNA segment was cloned into pGEX-4T-3, an Escherichia coli expression vector, and it's expression was confirmed by Western-blot analysis.