Journal of Microbiology

Journal Article

Cyclobacterium sediminis sp. nov. isolated from a sea cucumber aquaculture farm and emended description of the genus Cyclobacterium: Seyeon Shin , Hyung-Yeel Kahng; J. Microbiol. 2017;55(2):90-95. Published online January 26, 2017; DOI: https://doi.org/10.1007/s12275-017-6484-6

46 View
0 Download
5 Crossref

An aerobic, Gram-negative bacterium, designated strain SD70T, was isolated from sea cucumber aquaculture farm sediment in Taean, Korea, and its taxonomic status was established by undertaking a polyphasic study. Cells of strain SD70T were non-motile, catalase-, and oxidase-positive, nonspore- forming, and horseshoe-shaped. Optimal growth was observed under 25–30°C, pH 7.0–8.0, and 3.0–5.0% (w/v) NaCl conditions. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SD70T fell within an evolutionary group comprising species of the genus Cyclobacterium. Strain SD70T shared 92.1–98.5% 16S rRNA sequence similarity values with the type strains of species of the genus Cyclobacterium. Relatively low levels of DNA-DNA relatedness were found between strain SD70T and C. marinum DSM 745T (40.2%) and C. amurskyense KMM 6143T (15.8%). The predominant cellular fatty acids were iso-C15:0 (32.1%), and anteiso-C15:0 (9.1%). Menaquinone MK-7 was the only respiratory quinone. The G+C content of the genomic DNA was 36 mol%. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and seven unidentified lipids. On the basis of phenotypic and genotypic data, strain SD70T represents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium sediminis sp. nov. is proposed. An emended description of the genus Cyclobacterium is also provided.

Citations

Citations to this article as recorded by

Taxogenomics of the Genus Cyclobacterium: Cyclobacterium xiamenense and Cyclobacterium halophilum as Synonyms and Description of Cyclobacterium plantarum sp. nov.
Azadeh Shahinpei, Mohammad Ali Amoozegar, Leila Mirfeizi, Mahdi Moshtaghi Nikou, Antonio Ventosa, Cristina Sánchez-Porro
Microorganisms.2020; 8(4): 610. CrossRef
Cyclobacterium salsum sp. nov. and Cyclobacterium roseum sp. nov., isolated from a saline lake
Yong-Hong Liu, Yuan-Guo Xie, Li Li, Hong-Chen Jiang, Osama Abdalla Abdelshafy Mohamad, Wael Hozzein, Bao-Zhu Fang, Wen-Jun Li
International Journal of Systematic and Evolutionary Microbiology .2020; 70(6): 3785. CrossRef
Glacial-interglacial transitions in microbiomes recorded in deep-sea sediments from the western equatorial Atlantic
Lucas Freitas, Luciana Appolinario, Gabriela Calegario, Mariana Campeão, Diogo Tschoeke, Gizele Garcia, Igor Martins Venancio, Carlos A.N. Cosenza, Luciana Leomil, Marcelo Bernardes, Ana Luiza Albuquerque, Cristiane Thompson, Fabiano Thompson
Science of The Total Environment.2020; 746: 140904. CrossRef
Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes
Marina García-López, Jan P. Meier-Kolthoff, Brian J. Tindall, Sabine Gronow, Tanja Woyke, Nikos C. Kyrpides, Richard L. Hahnke, Markus Göker
Frontiers in Microbiology.2019;[Epub] CrossRef
Notification of changes in taxonomic opinion previously published outside the IJSEM
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology .2017; 67(7): 2081. CrossRef

Research Support, Non-U.S. Gov't

Citrimicrobium luteum gen. nov., sp. nov., Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Gut of a Sea Cucumber Stichopus japonicus: Hong-Joo Jung , In-Tae Cha , Kyung June Yim , Hye Seon Song , Kichul Cho , Daekyung Kim , Hae-Won Lee , Jae Kook Lee , Myung-Ji Seo , Seong Woon Roh , Sung-Jae Lee; J. Microbiol. 2014;52(10):819-824. Published online August 27, 2014; DOI: https://doi.org/10.1007/s12275-014-4136-7

46 View
0 Download
1 Crossref

Abstract

A Gram-stain negative, yellow-pigmented, motile, pleomorphic bacterium, designated strain CBA4602T, was isolated from the gut of the sea cucumber Stichopus japonicus, which was collected from Jeju Island in the Republic of Korea. In a phylogenetic analysis based on the 16S rRNA gene, strain CBA4602T belonged to the order Sphingomonadales in the class Alphaproteobacteria. The 16S rRNA gene sequence similarity between strain CBA4602T and ‘Citromicrobium bathyomarinum’ JF-1, the most closely related strain having nonvalidly published name, was 98.4%, followed by 95.2–96.7% identities with sequence of the other closest strains in the genus Erythrobacter. Strain CBA4602T had bacteriochlorophyll a and carotenoids. Strain CBA4602T grew in 0–10% (w/v) NaCl, at 10–42°C and pH 6.0–8.0, with optimal growth in 1–2% NaCl, at 30–37°C and pH 7.0. Strain CBA4602T was positive for catalase and oxidase activities and was able to hydrolyse gelatine and Tween 20 and 40, but not starch, Tween 80 or L-tyrosine. The G+C content of genomic DNA from strain CBA4602T was 68.0 mol% and Q-10 was the major detected isoprenoid quinone. The polar lipids were three unidentified phospholipids, three unidentified glycolipids, and two unidentified lipids. The dominant fatty acids were anteiso-C15:0, C16:0, anteiso-C17:0 and C18:0. As considering the current taxonomic status of the genus ‘Citromicrobium’ and polyphasic taxonomic analyses, strain CBA4602T represents a novel genus and species. The name Citrimicrobium luteum is proposed for the type strain CBA4602T (=KACC 17668T =JCM 19530T).

Citations

Citations to this article as recorded by

Association of aerobic anoxygenic phototrophs and zebra mussels, Dreissena polymorpha, within the littoral zone of Lake Winnipeg
Steven Brady Kuzyk, Kaitlyn Wiens, Xiao Ma, Vladimir Yurkov
Journal of Great Lakes Research.2021; 47(3): 567. CrossRef

First
Prev
Page of 1
Next
Last

Search