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- Comparative and bioinformatics analyses of pathogenic bacterial secretomes identified by mass spectrometry in Burkholderia species
- Thao Thi Nguyen , Tae-Soo Chon , Jaehan Kim , Young-Su Seo , Muyoung Heo
- J. Microbiol. 2017;55(7):568-582. Published online June 30, 2017
- DOI: https://doi.org/10.1007/s12275-017-7085-0
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Abstract
- Secreted proteins (secretomes) play crucial roles during bacterial pathogenesis in both plant and human hosts. The identification and characterization of secretomes in the two plant pathogens Burkholderia glumae BGR1 and B. gladioli BSR3, which cause diseases in rice such as seedling blight, panicle blight, and grain rot, are important steps to not only understand the disease-causing mechanisms but also find remedies for the diseases. Here, we identified two datasets of secretomes in B. glumae BGR1 and B. gladioli BSR3, which consist of 118 and 111 proteins, respectively, using mass spectrometry approach and literature curation. Next, we characterized the functional properties, potential secretion pathways and sequence information properties of secretomes of two plant pathogens in a comparative analysis by various computational approaches. The ratio of potential non-classically secreted proteins (NCSPs) to classically secreted proteins (CSPs) in B. glumae BGR1 was greater than that in B. gladioli BSR3. For CSPs, the putative hydrophobic regions (PHRs) which are essential for secretion process of CSPs were screened in detail at their N-terminal sequences using hidden Markov model (HMM) – based method. Total 31 pairs of homologous proteins in two bacterial secretomes were indicated based on the global alignment (identity ≥ 70%). Our results may facilitate the understanding of the species-specific features of secretomes in two plant pathogenic Burkholderia species.
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Citations
Citations to this article as recorded by
- Proteomics approaches: A review regarding an importance of proteome analyses in understanding the pathogens and diseases
Muhammad Zubair, Jia Wang, Yanfei Yu, Muhammad Faisal, Mingpu Qi, Abid Ullah Shah, Zhixin Feng, Guoqing Shao, Yu Wang, Qiyan Xiong
Frontiers in Veterinary Science.2022;[Epub] CrossRef - Characterisation of Pythium aristosporum Oomycete—A Novel Pathogen Causing Rice Seedling Blight in China
Jinxin Liu, Ruisi Zhang, Chuzhen Xu, Chunlai Liu, Yanyan Zheng, Xue Zhang, Shasha Liu, Yonggang Li
Journal of Fungi.2022; 8(9): 890. CrossRef - Bacterial Panicle Blight and Burkholderia glumae: From Pathogen Biology to Disease Control
Laura Ortega, Clemencia M. Rojas
Phytopathology®.2021; 111(5): 772. CrossRef
- Proteomics approaches: A review regarding an importance of proteome analyses in understanding the pathogens and diseases
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