MSK
Search
- Page Path
- HOME > Search
Journal Article
- Promoter exchange of the cryptic nonribosomal peptide synthetase gene for oligopeptide production in Aspergillus oryzae
- Chanikul Chutrakul , Sarocha Panchanawaporn , Sukanya Jeennor , Jutamas Anantayanon , Kobkul Laoteng
- J. Microbiol. 2022;60(1):47-56. Published online November 9, 2021
- DOI: https://doi.org/10.1007/s12275-022-1442-3
- 52 View
- 0 Download
- 6 Web of Science
- 6 Crossref
-
Abstract
-
Oligopeptides with functional activities are of current interest
in the nutraceutical and medical sectors. The development of
the biosynthetic process of oligopeptides through a nonribosomal
peptide synthetase (NRPS) system has become more
challenging. To develop a production platform for nonribosomal
peptides (NRPs), reprogramming of transcriptional
regulation of the acv gene encoded ACV synthetase (ACVS)
was implemented in Aspergillus oryzae using the CRISPRCas9
system. Awakening silent acv expression was successfully
achieved by promoter substitution. Among the three exchanged
promoters, AoPgpdA, AoPtef1, and PtPtoxA, the
replacement of the native promoter with AoPgpdA led to the
highest ACV production in A. oryzae. However, the ACV production
of the AoPGpdA strain was also dependent on the
medium composition, in which urea was the best nitrogen
source, and a C:N ratio of 20:1 was optimal for tripeptide production.
In addition to cell growth, magnesium ions are an
essential element for ACV production and might participate
in ACVS activity. It was also found that ACV was the growthassociated
product of the engineered strain that might be a
result
of constitutive transcriptional control by the AoPgpdA promoter. This study offers a potential strategy for nonribosomal ACV production using the fungal system, which is applicable for redesigning bioactive oligopeptides with industrial relevance. -
Citations
Citations to this article as recorded by
- Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in Aspergillus oryzae
Xiao Jia, Jiayi Song, Yijian Wu, Sai Feng, Zeao Sun, Yan Hu, Mengxue Yu, Rui Han, Bin Zeng
Journal of Fungi.2024; 10(5): 312. CrossRef - Transcriptome-based Mining of the Constitutive Promoters for Tuning Gene Expression in Aspergillus oryzae
Kobkul Laoteng, Jutamas Anantayanon, Chanikul Chutrakul, Sarocha Panchanawaporn, Sukanya Jeennor
Journal of Microbiology.2023; 61(2): 199. CrossRef - Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform
Sukanya Jeennor, Jutamas Anantayanon, Sarocha Panchanawaporn, Chanikul Chutrakul, Wanwipa Vongsangnak, Kobkul Laoteng
Microbial Cell Factories.2023;[Epub] CrossRef - Synthetic microbes and biocatalyst designs in Thailand
Duangthip Trisrivirat, Ruchanok Tinikul, Pimchai Chaiyen
Biotechnology Notes.2023; 4: 28. CrossRef - Potential of Aspergillus oryzae as a biosynthetic platform for indigoidine, a non-ribosomal peptide pigment with antioxidant activity
Sarocha Panchanawaporn, Chanikul Chutrakul, Sukanya Jeennor, Jutamas Anantayanon, Nakul Rattanaphan, Kobkul Laoteng, Daniel Cullen
PLOS ONE.2022; 17(6): e0270359. CrossRef - CRISPR/Cas9-Based Genome Editing and Its Application in Aspergillus Species
Feng-Jie Jin, Bao-Teng Wang, Zhen-Dong Wang, Long Jin, Pei Han
Journal of Fungi.2022; 8(5): 467. CrossRef
- Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in Aspergillus oryzae
First
Prev
TOP
