Journal of Microbiology

Journal Articles

Prevalence and characteristics of the mcr-1 gene in retail meat samples in Zhejiang Province, China: Biao Tang , Jiang Chang , Yi Luo , Han Jiang , Canying Liu , Xingning Xiao , Xiaofeng Ji , Hua Yang; J. Microbiol. 2022;60(6):610-619. Published online March 31, 2022; DOI: https://doi.org/10.1007/s12275-022-1597-y

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Considering the serious threat to food safety and public health posed by pathogens with colistin resistance, colistin was banned as a growth promoter in 2017 in China. In recent years, the resistance rate of Escherichia coli isolated from animal intestines or feces to colistin has decreased. However, the prevalence and characteristics of the mcr-1 gene in retail meat have not been well explored. Herein, 106 mcr-1-negative and 16 mcr- 1-positive E. coli isolates were randomly recovered from 120 retail meat samples and screened using colistin. The 106 E. coli isolates showed maximum resistance to sulfafurazole (73.58%) and tetracycline (62.26%) but susceptibility to colistin (0.00%). All 16 mcr-1-positive E. coli isolates showed resistance to colistin, were extended spectrum beta-lactamase (ESBL)-positive and exhibited complex multidrug resistance (MDR). For these 16 isolates, 17 plasmid replicons and 42 antibiotic resistance genes were identified, and at least 7 antibiotic resistance genes were found in each isolate. Acquired disinfectant resistance genes were identified in 75.00% (12/16) of the isolates. Furthermore, comparative genomic and phylogenetic analysis
results
indicated that these 16 mcr-1-positive E. coli isolates and the most prevalent mcr-1-harboring IncI2 plasmid in this study were closely related to other previously reported mcr-1-positive E. coli isolates and the IncI2 plasmid, respectively, showing their wide distribution. Taken together, our findings showed that retail meat products were a crucial reservoir of mcr-1 during the colistin ban period and should be continuously monitored.

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Regression models from portable NIR spectra for predicting the carcass traits and meat quality of beef cattle
Nathália Veloso Trópia, Rizielly Saraiva Reis Vilela, Flávia Adriane de Sales Silva, Dhones Rodrigues Andrade, Adailton Camêlo Costa, Fernando Alerrandro Andrade Cidrini, Jardeson de Souza Pinheiro, Pauliane Pucetti, Mario Luiz Chizzotti, Sebastião de Cam
PLOS ONE.2024; 19(5): e0303946. CrossRef
IncHI1 plasmids mediated the tet(X4) gene spread in Enterobacteriaceae in porcine
Jiangang Ma, Juan Wang, Hua Yang, Mengru Su, Ruichao Li, Li Bai, Jie Feng, Yuting Huang, Zengqi Yang, Biao Tang
Frontiers in Microbiology.2023;[Epub] CrossRef
Prevalence and molecular characteristics of polymyxin-resistant Enterobacterales in a Chinese tertiary teaching hospital
Chenlu Xiao, Xuming Li, Lianjiang Huang, Huiluo Cao, Lizhong Han, Yuxing Ni, Han Xia, Zhitao Yang
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
Farm to table: colistin resistance hitchhiking through food
Absar Talat, Carla Miranda, Patrícia Poeta, Asad U. Khan
Archives of Microbiology.2023;[Epub] CrossRef
Detection of mcr-1-harbouring Escherichia coli by quantum dot labelling of synthetic small peptides mimicking lipopolysaccharide receptors
Chenghao Wang, Biao Tang, Jiusheng Wu, Xi Jin, Shuwen Ke, Hua Yang, Yuehuan Liu
International Journal of Antimicrobial Agents.2023; 62(3): 106898. CrossRef
Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China
Biao Tang, Juan Ni, Jiahui Lin, Yangying Sun, Hui Lin, Yuehong Wu, Hua Yang, Min Yue
Frontiers in Microbiology.2022;[Epub] CrossRef
Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China
Wei Zhou, Rumeng Lin, Zhijin Zhou, Jiangang Ma, Hui Lin, Xue Zheng, Jingge Wang, Jing Wu, Yuzhi Dong, Han Jiang, Hua Yang, Zhangnv Yang, Biao Tang, Min Yue
Frontiers in Microbiology.2022;[Epub] CrossRef
The Occurrence and Genomic Characteristics of mcr-1-Harboring Salmonella from Retail Meats and Eggs in Qingdao, China
Changan Li, Xiulei Gu, Liping Zhang, Yuqing Liu, Yan Li, Ming Zou, Baotao Liu
Foods.2022; 11(23): 3854. CrossRef

Hymenobacter jeollabukensis sp. nov., isolated from soil: Leonid N. Ten , Young Eun Han , Kyeung Il Park , In-Kyu Kang , Jeung-Sul Han , Hee-Young Jung; J. Microbiol. 2018;56(7):500-506. Published online June 28, 2018; DOI: https://doi.org/10.1007/s12275-018-8085-4

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Abstract

A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterial strain, designated 1-3-3-8T, was isolated from soil and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 1-3-3-8T belongs to the family Cytophagaceae of phylum Bacteroidetes and is most closely related to Hymenobacter paludis KBP-30T (96.8% similarity), Hymenobacter ocellatus Myx2105T (96.8%), Hymenobacter coalescens WW84T (95.6%), and Hymenobacter deserti ZLB-3T (95.4%). The G + C content of the genomic DNA of strain 1-3-3-8T was 63.6 mol%. The isolate contained C15:0 iso (28.4%), summed feature 4 (C17:1 anteiso B/C17:1 iso I; 18.9%), and C15:0 anteiso (17.6%) as major fatty acids, MK-7 as the predominant respiratory quinone, and sym-homospermidine as the predominant polyamine. The major polar lipids were phosphatidylethanolamine and an unidentified lipid. The phenotypic and chemotaxonomic data supported the affiliation of strain 1-3-3-8T with the genus Hymenobacter. The DNA-DNA relatedness between strain 1-3-3-8T and H. paludis KCTC 32237T and H. ocellatus DSM 11117T were 24.5 and 27.4% respectively, clearly showing that the isolate is not related to them at the species level. Overall, the novel strain could be differentiated from its phylogenetic neighbors on the basis of several phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 1-3-3-8T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter jeollabukensis sp. nov. has been proposed. The type strain is 1-3-3-8T (= KCTC 52741T = JCM 32192T).

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Isolation and characterization of two new species, Hymenobacter mellowenesis sp. nov. and Hymenobacter aranciens sp. nov., from soil
Seonjae Kim, Sathiyaraj Srinivasan, Myung Kyum Kim
Archives of Microbiology.2024;[Epub] CrossRef
Hymenobacter canadensis sp. nov., isolated from freshwater of the pond in Cambridge Bay, Canada
Woohyun Kim, Seonghan Jang, Namyi Chae, Mincheol Kim, Jung-Yong Yeh, Sanghee Kim, Yung Mi Lee
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Compositional Data Analysis of 16S rRNA Gene Sequencing Results from Hospital Airborne Microbiome Samples
Maria Rita Perrone, Salvatore Romano, Giuseppe De Maria, Paolo Tundo, Anna Rita Bruno, Luigi Tagliaferro, Michele Maffia, Mattia Fragola
International Journal of Environmental Research and Public Health.2022; 19(16): 10107. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology.2019; 69(1): 5. CrossRef
Hymenobacter oligotrophus sp. nov., isolated from a contaminated agar plate
Yingchao Geng, Yumin Zhang, Jin Tian, Jia Liu, Kun Qin, Yao Huang, Ziyan Wei, Fang Peng
Antonie van Leeuwenhoek.2019; 112(10): 1533. CrossRef
Hymenobacter edaphi sp. nov., isolated from abandoned arsenic-contaminated farmland soil
Li Nie, Xia Fan, Dongfang Xiang, Shuijiao Liao, Gejiao Wang
International Journal of Systematic and Evolutionary Microbiology.2019; 69(9): 2921. CrossRef

Proposal of three novel species of soil bacteria, Variovorax ureilyticus, Variovorax rhizosphaerae, and Variovorax robiniae, in the family Comamonadaceae: Tuan Manh Nguyen , Ngoc Hoang Trinh , Jaisoo Kim; J. Microbiol. 2018;56(7):485-492. Published online June 14, 2018; DOI: https://doi.org/10.1007/s12275-018-8025-3

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Abstract

Three novel bacterial strains (UCM-2T, UCM-G28T, and UCM-G35T) were obtained while isolating soil bacteria for the development of antibiotics. Cells of these strains were Gram-negative, non-spore forming, motile by means of a single flagellum, and rod shaped. In all strains, the predominant isoprenoid quinone was ubiquinone-8 (Q-8). Cells contained C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), and C17:0 cyclo as the major fatty acids, and C10:0 3-OH as the major hydroxy fatty acid. The polar lipid profiles of the three novel strains were dominated by diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The genomic DNA G + C contents of strains UCM-2T, UCM-G28T, and UCMG35T were 67.5, 65.9, and 66.4 mol%, respectively. Phylogenetic analyses based on 16S rRNA sequences showed that strain UCM-2T was most closely related to Variovorax soli NBRC 106424T, whereas strains UCM-G28T and UCM-G35T were most similar to Variovorax ginsengisoli Gsoil 3165T. Values indicating DNA-DNA hybridization between the novel isolates and closely related species in the genus Variovorax were lower than the 70% cut-off point. These phenotypic, chemotaxonomic, and phylogenetic data indicate that the three isolates should be classified as new members of the genus Variovorax, for which the names Variovorax ureilyticus sp. nov., Variovorax rhizosphaerae sp. nov., and Variovorax robiniae sp. nov. are proposed. The type strains are UCM-2T (= KACC 18899T = NBRC 112306T), UCMG28T (= KACC 18900T = NBRC 112307T), and UCM-G35T (= KACC 18901T = NBRC 112308T), respectively.

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Encephalartos natalensis association with non-cyanobacterial species increased reliance on atmospheric nitrogen in acidic and nutrient-deficient savanna woodland ecosystem soils
Siphelele Ndlovu, Nqobile Motsomane, Terence Suinyuy, Anathi Magadlela
Transactions of the Royal Society of South Africa.2024; : 1. CrossRef
The first reported case of human infection with Variovorax durovernensis; a novel Variovorax sp. Isolated from the prosthetic aortic graft of a shepherd
Lara Payne, Adela Alcolea-Medina, Luke B Snell, Christopher Alder, Themoula Charalampous, Jake D Turnbull, Jonathan D Edgeworth, Rahul Batra, John L Klein, Anna L Goodman
Clinical Infection in Practice.2024; 21: 100332. CrossRef
Encephalartos natalensis, Their Nutrient-Cycling Microbes and Enzymes: A Story of Successful Trade-Offs
Siphelele Ndlovu, Terence N. Suinyuy, María A. Pérez-Fernández, Anathi Magadlela
Plants.2023; 12(5): 1034. CrossRef
Variovorax durovernensis sp. nov., a novel species isolated from an infected prosthetic aortic graft in a human
Adela Alcolea-Medina, Luke B. Snell, Lara Payne, Christopher J. Alder, Jake D. Turnbull, Themoula Charalampous, Lisa Bryan, John L. Klein, Jonathan D. Edgeworth, Rahul Batra, Anna L. Goodman
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Variovorax terrae sp. nov. Isolated from Soil with Potential Antioxidant Activity
Chae Yung Woo, Jaisoo Kim
Journal of Microbiology and Biotechnology.2022; 32(7): 855. CrossRef
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Phytobiomes Journal.2021; 5(1): 1. CrossRef
Nitrogen Fertilization Reduces Nitrogen Fixation Activity of Diverse Diazotrophs in Switchgrass Roots
Rahul A. Bahulikar, Srinivasa R. Chaluvadi, Ivone Torres-Jerez, Jagadish Mosali, Jeffrey L. Bennetzen, Michael Udvardi
Phytobiomes Journal.2021; 5(1): 80. CrossRef
Variovorax beijingensis sp. nov., a novel plant-associated bacterial species with plant growth-promoting potential isolated from different geographic regions of Beijing, China
Jun-lian Gao, Yu-chen Sun, Jing Xue, Pengbo Sun, Hui Yan, Mohammad Sayyar Khan, Li-wei Wang, Xiuhai Zhang, Jian-guang Sun
Systematic and Applied Microbiology.2020; 43(6): 126135. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology.2018; 68(11): 3379. CrossRef

Larkinella roseus sp. nov., a species of the family Cytophagaceae isolated from beach soil: Jae-Bong Lee , Sumin Hong , Seung-Yeol Lee , Su-Jin Park , Kyeung Il Park , Seok-Gwan Choi , Myung Kyum Kim , Leonid N. Ten , Hee-Young Jung; J. Microbiol. 2018;56(1):30-35. Published online January 4, 2018; DOI: https://doi.org/10.1007/s12275-018-7476-x

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Abstract

The taxonomic position of bacterial strain, designated 15J16- 1T3AT, recovered from a soil sample was established using a polyphasic approach. Phylogenic analysis based on the 16S rRNA gene sequence showed that strain 15J16-1T3AT belonged to the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to ‘Larkinella harenae’ 15J9-9 (95.9% similarity), Larkinella ripae 15J11-11T (95.6%), Larkinella bovis M2TB15T (94.7%), Larkinella arboricola Z0532T (93.9%), and Larkinella insperata LMG 22510T (93.5%). Cells were rod-shaped, Gram-stain-negative, aerobic, and nonmotile. The isolate grew on NA, R2A, TSA, but not on LB agar. The strain was able to grow at temperature range from 10°C to 30°C with an optimum at 25°C and pH 6–8. Menaquinone MK-7 was the predominant respiratory quinone. The major cellular fatty acids comprised C16:1 ω5c (48.6%) and C15:0 iso (24.1%). Phosphatidylethanolamine, phosphatidylserine, and an unidentified lipid were the major polar lipids. The G + C content of the genomic DNA was 49.5 mol%. Strain 15J16-1T3AT could be distinguished from its closest phylogenetic neighbors based on its phenotypic, genotypic, and chemotaxonomic features. Therefore, the isolate is considered to represent a novel species in the genus Larkinella, for which the name Larkinella roseus sp. nov. is proposed. The type strain is 15J16-1T3AT (= KCTC 52004T = JCM 31991T).

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Larkinella humicola sp. nov., a gamma radiation-resistant bacterium isolated from soil
Yuna Park, Leonid N. Ten, Young Koung Lee, Hee‑Young Jung, Myung Kyum Kim
Archives of Microbiology.2022;[Epub] CrossRef
Larkinella punicea sp. nov., isolated from manganese mine soil
Zijie Zhou, Lin Zhu, Yixuan Dong, Xian Xia, Shijuan Wu, Gejiao Wang
Archives of Microbiology.2020; 202(9): 2517. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George Garrity
International Journal of Systematic and Evolutionary Microbiology.2018; 68(5): 1411. CrossRef
Hymenobacter segetis sp. nov., isolated from soil
Leonid N. Ten, Soo Jeong Lim, Byung-Oh Kim, In-Kyu Kang, Hee-Young Jung
Archives of Microbiology.2018; 200(8): 1167. CrossRef

Spirosoma flavus sp. nov., a novel bacterium from soil of Jeju Island: Nabil Elderiny , Seung-Yeol Lee , Sangkyu Park , In-Kyu Kang , Myung Kyum Kim , Dae Sung Lee , Leonid N. Ten , Hee-Young Jung; J. Microbiol. 2017;55(11):850-855. Published online October 27, 2017; DOI: https://doi.org/10.1007/s12275-017-7360-0

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Abstract

A novel, Gram-staining negative, yellow pigmented bacterial strain, designated 15J11-2T, was isolated from soil sample on Jeju Island, Republic of Korea. The strain was subjected to a taxonomic study using a polyphasic approach. The strain was able to grow at temperature range from 10°C to 30°C, pH 7–8, and in presence of 0–1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain 15J11-2T belongs to the genus Spirosoma and levels of 16S rRNA gene sequence similarity ranged from 91.5% to 89.8%. The genomic DNA G + C content of strain 15J11-2T was 46.0 mol%. The isolate contained phosphatidylethanolamine and an unidentified aminophospholipid as the main polar lipids, menaquinone MK-7 as the predominant respiratory quinone, and summed feature 3 (C16:1 ω6c/C16:1 ω7c; 39.4%), C16:1 ω5c (27.1%), and C16:0 (13.0%) as the major fatty acids, which supported the affiliation of strain 15J11-2T to the genus Spirosoma. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J11-2T from recognized Spirosoma species. On the basis of its phenotypic properties, genotypic distinctiveness, chemotaxonomic features, strain 15J11-2T represents a novel species of the genus Spirosoma, for which the name Spirosoma flavus sp. nov. is proposed. The type strain is 15J11-2T (= KCTC 52026T = JCM 31998T).

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Spirosoma profusum sp. nov., and Spirosoma validum sp. nov., radiation-resistant bacteria isolated from soil in South Korea
Yuna Park, Soohyun Maeng, Tuvshinzaya Damdintogtokh, Jing Zhang, Min-Kyu Kim, Sathiyaraj Srinivasan, Myung Kyum Kim
Antonie van Leeuwenhoek.2021; 114(7): 1155. CrossRef

Research Support, Non-U.S. Gov'ts

Antibacterial potential of a small peptide from Bacillus sp. RPT-0001 and its capping for green synthesis of silver nanoparticles: Supriya Deepak Patil , Rajnikant Sharma , Tapas Bhattacharyya , Piyush Kumar , Manasi Gupta , Bhupinder Singh Chaddha , Naveen Kumar Navani , Ranjana Pathania; J. Microbiol. 2015;53(9):643-652. Published online August 1, 2015; DOI: https://doi.org/10.1007/s12275-015-4686-3

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Abstract

Infirmity and death from diseases caused by unsafe food are a continual hazard to communal health safety and socio-economic growth throughout the world. Chemical preservatives are associated with health hazards and toxicity issues. In the study reported here, 200 soil isolates from Western Himalayan region in India were screened for potential antibacterial activity against food-borne pathogens. This study led to the isolation of a bacterial strain belonging to the Genus Bacillus and was designated as RPT-0001. The associated antibacterial activity was sensitive to pronase E treatment. Bioassay-guided fractionation using reverse phase high performance liquid chromatography (RP-HPLC) led to isolation of the antibacterial peptide designated as RPT-0001. The molecular weight of RPT-0001 was determined by electro- spray ionization mass spectroscopy (ESI-MS) as 276.9 Da. RPT-0001 was inhibitory to both Gram-negative and Grampositive food-borne bacteria tested. The characteristics of RPT-0001 do not match with that of any other known antibacterial peptides produced by Bacillus sp. or related genera. Purified RPT-0001 was successfully used in synthesis of silver nanoparticles effective against food-borne pathogenic bacteria. The antibacterial peptide and silver nanoparticles synthesized utilizing it as a capping and reducing agent hold promising potential in food preservation, in packaging material and as a therapeutic agent in the treatment of foodborne infections.

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Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from Lactobacillus acidophilus-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities
Arif Jamal Siddiqui, Mitesh Patel, Mohd Adnan, Sadaf Jahan, Juhi Saxena, Mohammed Merae Alshahrani, Abdelmushin Abdelgadir, Fevzi Bardakci, Manojkumar Sachidanandan, Riadh Badraoui, Mejdi Snoussi, Allal Ouhtit
Pharmaceutics.2023; 15(2): 403. CrossRef
Purification and characterization of bacteriocin Bac23 extracted from Lactobacillus plantarum PKLP5 and its interaction with silver nanoparticles for enhanced antimicrobial spectrum against food-borne pathogens
Parveen Kaur Sidhu, Kiran Nehra
LWT.2021; 139: 110546. CrossRef
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Keyur Bhatt, Anita Kongor, Mohd Atharb, Vinod Jain
Journal of Metals, Materials and Minerals.2021; 31(3): 1. CrossRef
Physicochemical-guided design of cathelicidin-derived peptides generates membrane active variants with therapeutic potential
Nelson G. J. Oliveira, Marlon H. Cardoso, Nadya Velikova, Marcel Giesbers, Jerry M. Wells, Taia M. B. Rezende, Renko de Vries, Octávio L. Franco
Scientific Reports.2020;[Epub] CrossRef
Bacteriocin‐capped silver nanoparticles for enhanced antimicrobial efficacy against food pathogens
Parveen Kaur Sidhu, Kiran Nehra
IET Nanobiotechnology.2020; 14(3): 245. CrossRef
Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review
Hidayat Mohd Yusof, Rosfarizan Mohamad, Uswatun Hasanah Zaidan, Nor’ Aini Abdul Rahman
Journal of Animal Science and Biotechnology.2019;[Epub] CrossRef
Advances in Lipid and Metal Nanoparticles for Antimicrobial Peptide Delivery
Marcin Makowski, Ítala C. Silva, Constança Pais do Amaral, Sónia Gonçalves, Nuno C. Santos
Pharmaceutics.2019; 11(11): 588. CrossRef
The therapeutic applications of antimicrobial peptides (AMPs): a patent review
Hee-Kyoung Kang, Cheolmin Kim, Chang Ho Seo, Yoonkyung Park
Journal of Microbiology.2017; 55(1): 1. CrossRef
Bacilli as Biological Nano-factories Intended for Synthesis of Silver Nanoparticles and Its Application in Human Welfare
Varish Ahmad, Qazi Mohammad Sajid Jamal, Arun K. Shukla, Javed Alam, Ahamad Imran, Usama Mohamed Abaza
Journal of Cluster Science.2017; 28(4): 1775. CrossRef
High-resolution imaging of the microbial cell surface
Ki Woo Kim
Journal of Microbiology.2016; 54(11): 703. CrossRef

Massilia kyonggiensis sp. nov., Isolated from Forest Soil in Korea: Jaisoo Kim; J. Microbiol. 2014;52(5):378-383. Published online May 9, 2014; DOI: https://doi.org/10.1007/s12275-014-4010-7

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Abstract

A Gram-negative, short, rod-shaped bacterium, TSA1T, was isolated from forest soil collected at Kyonggi University, South Korea. Assessment of 16S rRNA gene sequence sim-ilarity indicated that the strain is related to Massilia niastensis 5516S-1T (98.3%), M. haematophila CCUG 38318T (97.9%), M. aerilata 5516S-11T (97.9%), M. tieshanensis TS3T (97.6%), and M. varians CCUG 3529T (97.1%). Colonies grown on Reasoner’s 2A agar at 30°C for 2 days were transparent, white, round, smooth, and glossy. The cells grew at 10–42°C (optimum: 25–37°C) and pH 5–9 (optimum: 5–9) and in 0–2% NaCl (optimum: 0–1%). TSA1T was able to grow on trypticase soy and nutrient agar, but not on Luria-Bertani or MacConkey agar. The strain was catalase- and oxidase- positive and able to degrade starch and casein, but not car-boxymethyl cellulose. The predominant quinone of TSA1T was Q-8, the major fatty acids were summed feature 3 and C16:0, and the DNA G+C content was 66.7 mol%. Given these findings, we propose that this strain is a novel species of the genus Massilia. We suggest the name Massilia kyonggiensis sp. nov. (type strain, KACC 17471T =KEMB 9005-031T =JCM 19189T).

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Massilia luteola sp. nov., a novel indole-producing and cellulose-degrading bacterium isolated from soil
Xinbing Huang, Shengtao Qi, Wenshuai Song, Xiaoxin Yu, Haifeng Zhang, Wensheng Xiang, Junwei Zhao, Xiangjing Wang
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef
Genome-wide and constrained ordination-based analyses of EC code data support reclassification of the species of Massilia La Scola et al. 2000 into Telluria Bowman et al. 1993, Mokoshia gen. nov. and Zemynaea gen. nov.
John P. Bowman
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Cold-shock gene cspC in the genome of Massilia polaris sp. nov. revealed cold-adaptation
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Dong-Uk Kim, Jaisoo Kim
Antonie van Leeuwenhoek.2021; 114(8): 1275. CrossRef
Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
Scientific Reports.2021;[Epub] CrossRef
Massilia rhizosphaerae sp. nov., a rice-associated rhizobacterium with antibacterial activity against Ralstonia solanacearum
Chenxu Li, Peng Cao, Chuanjiao Du, Xue Zhang, Hui Bing, Lei Li, Peng Sun, Wensheng Xiang, Junwei Zhao, Xiangjing Wang
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef
Large Blooms of Bacillales ( Firmicutes ) Underlie the Response to Wetting of Cyanobacterial Biocrusts at Various Stages of Maturity
Ulas Karaoz, Estelle Couradeau, Ulisses Nunes da Rocha, Hsiao-Chien Lim, Trent Northen, Ferran Garcia-Pichel, Eoin L. Brodie, Mark J. Bailey
mBio.2018;[Epub] CrossRef
Massilia variansIsolated from a Clinical Specimen
Jooyoung Cho, Keon-Han Kim, Jung Ok Kim, Jun Sung Hong, Seok Hoon Jeong, Kyungwon Lee
Infection & Chemotherapy.2017; 49(3): 219. CrossRef
Massilia phosphatilytica sp. nov., a phosphate solubilizing bacteria isolated from a long-term fertilized soil
Bang-Xiao Zheng, Qing-Fang Bi, Xiu-Li Hao, Guo-Wei Zhou, Xiao-Ru Yang
International Journal of Systematic and Evolutionary Microbiology.2017; 67(8): 2514. CrossRef
Massilia neuiana sp. nov., isolated from wet soil
Xin Zhao, Xuejie Li, Nan Qi, Meijun Gan, Yujin Pan, Tianfang Han, Xiaomin Hu
International Journal of Systematic and Evolutionary Microbiology.2017; 67(12): 4943. CrossRef
Massilia agri sp. nov., isolated from reclaimed grassland soil
Dhiraj Kumar Chaudhary, Jaisoo Kim
International Journal of Systematic and Evolutionary Microbiology.2017; 67(8): 2696. CrossRef
Massilia psychrophila sp. nov., isolated from an ice core
Bixi Guo, Yongqin Liu, Zhengquan Gu, Liang Shen, Keshao Liu, Ningliang Wang, Tingting Xing, Hongcan Liu, Yuguang Zhou, Jiule Li
International Journal of Systematic and Evolutionary Microbiology.2016; 66(10): 4088. CrossRef
Massilia pinisoli sp. nov., isolated from forest soil
Khulan Altankhuu, Jaisoo Kim
International Journal of Systematic and Evolutionary Microbiology.2016; 66(9): 3669. CrossRef
Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing
Guang-Da Feng, Song-Zhen Yang, Hua-Ping Li, Hong-Hui Zhu
International Journal of Systematic and Evolutionary Microbiology.2016; 66(1): 50. CrossRef
Efficient biodegradation of phenanthrene by a novel strain Massilia sp. WF1 isolated from a PAH-contaminated soil
Haizhen Wang, Jun Lou, Haiping Gu, Xiaoyan Luo, Li Yang, Laosheng Wu, Yong Liu, Jianjun Wu, Jianming Xu
Environmental Science and Pollution Research.2016; 23(13): 13378. CrossRef
Massilia arvi sp. nov., isolated from fallow-land soil previously cultivated with Brassica oleracea, and emended description of the genus Massilia
Hina Singh, Juan Du, KyungHwa Won, Jung-Eun Yang, ChangShik Yin, MooChang Kook, Tae-Hoo Yi
International Journal of Systematic and Evolutionary Microbiology .2015; 65(Pt_10): 3690. CrossRef
Characterizing microbial communities dedicated for conversion of coal to methane in situ and ex situ
Ji Zhang, Yanna Liang, Rohit Pandey, Satya Harpalani
International Journal of Coal Geology.2015; 146: 145. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology .2014; 64(Pt_9): 2927. CrossRef

Isolation of Paenibacillus pinesoli sp. nov. from Forest Soil in Gyeonggi-Do, Korea: Jeongsuk Moon , Jaisoo Kim; J. Microbiol. 2014;52(4):273-277. Published online March 29, 2014; DOI: https://doi.org/10.1007/s12275-014-3622-2

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Abstract

Using a new culture method for unculturable soil bacteria, strain NB5T was isolated from forest soil at Kyonggi University, and characterized taxonomically on the basis of 16S rRNA gene sequence as well as phenotypic and chemotaxonomic characteristics. The novel strain was a Gram- and catalase-positive, rod-shaped bacterium, which grew in the pH range 6.0–9.5 (optimum, 6.5–9.5) and at temperatures between 15°C and 45°C (optimum, 25–40°C). Growth was possible at 0–5% NaCl (optimum, 0% to 3%) in nutrient, Luria-Bertani, and trypticase soy broths (TSB), as well as R2A medium (with optimal growth in TSB). A phylogenetic analysis of the 16S rRNA gene sequence showed that the novel strain was affiliated with the genus Paenibacillus and had 96.8% and 96.5% similarity to P. nanensis MX2-3T and P. agaridevorans DSM 1355T, respectively. The predominant menaquinone in NB5T was MK-7; the major fatty acids were anteiso-C15:0 and iso-C16:0; and the DNA G+C content was 54.5 mol%. We propose this strain as a novel species of the genus Paenibacillus, and suggest the name Paenibacillus pinesoli sp. nov. (type strain, KACC 17472T =KEMB 9005-025T =JCM 19203T).

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Monitoring of Soil Bacterial Community and Some Inoculated Bacteria After Prescribed Fire in Microcosm: Hong-Gyu Song , Ok-Sun Kim , Jae-Jun Yoo , Sun-Ok Jeon , Sun-Hee Hong , Dong-Hun Lee , Tae-Seok Ahn; J. Microbiol. 2004;42(4):285-291.; DOI: https://doi.org/2109 [pii]

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Abstract: The soil bacterial community and some inoculated bacteria were monitored to assess the microbial responses to prescribed fire in their microcosm. An acridine orange direct count of the bacteria in the unburned control soil were maintained at a relatively stable level (2.0~2.7 x10^9 cells/g^-1 . soil) during the 180 day study period. The number of bacteria in the surface soil was decreased by fire, but was restored after 3 months. Inoculation of some bacteria increased the number of inoculated bacteria several times and these elevated levels lasted several months. The ratios of eubacteria detected by a fluorescent in situ hybridization (FISH) method to direct bacterial count were in the range of 60~80% during the study period, with the exception of some lower values at the beginning, but there were no definite differences between the burned and unburned soils or the inoculated and uninoculated soils. In the unburned control soil, the ratios of [alpha]-, [beta]- and [gamma]-subgroups of the proteobacteria, Cytophaga-Flavobacterium and other eubacteria groups to that of the entire eubacteria were 13.7, 31.7, 17.1, 16.8 and 20.8%, respectively, at time 0. The overall change on the patterns of the ratios of the 5 subgroups of eubacteria in the uninoculated burned and inoculated soils were similar to those of the unburned control soil, with the exception of some minor variations during the initial period. The proportions of each group of eubacteria became similar in the different microcosms after 6 months, which may indicate the recovery of the original soil microbial community structure after fire or the inoculation of some bacteria. The populations of Azotobacter vinelandii, Bacillus megaterium and Pseudomonas fluorescens, which had been inoculated to enhance the microbial activities, and monitored by FISH method, showed similar changes in the microcosms, and maintained high levels for several months.

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