Research Support, U.S. Gov't, Non-P.H.S.
- Functional Shifts in Unvegetated, Perhumid, Recently-Deglaciated Soils Do Not Correlate with Shifts in Soil Bacterial Community Composition
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Sarah R. Sattin , Cory C. Cleveland , Eran Hood , Sasha C. Reed , Andrew J. King , Steven K. Schmidt , Michael S. Robeson , Nataly Ascarrunz , Diana R. Nemergut
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J. Microbiol. 2009;47(6):673-681. Published online February 4, 2010
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DOI: https://doi.org/10.1007/s12275-009-0194-7
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Abstract
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Past work in recently deglaciated soils demonstrates that microbial communities undergo shifts prior to plant colonization. To date, most studies have focused on relatively ‘long’ chronosequences with the ability to sample plant-free sites over at least 50 years of development. However, some recently deglaciated soils feature rapid plant colonization and questions remain about the relative rate of change in the microbial community in the unvegetated soils of these chronosequences. Thus, we investigated the forelands of the Mendenhall Glacier near Juneau, AK, USA, where plants rapidly establish. We collected unvegetated samples representing soils that had been ice-free for 0, 1, 4, and 8 years. Total nitrogen (N) ranged from 0.00~0.14 mg/g soil, soil organic carbon pools ranged from 0.6~2.3 mg/g soil, and both decreased in concentration between the 0 and 4 yr soils. Biologically available phosphorus (P) and pH underwent similar dynamics. However, both pH and available P increased in the 8 yr soils. Nitrogen fixation was nearly undetectable in the most recently exposed soils, and increased in the 8 yr soils to ~5 ng N fixed/cm2/h, a trend that was matched by the activity of the soil N-cycling enzymes urease and β-1,4-N-acetyl-glucosaminidase. 16S rRNA gene clone libraries revealed no significant differences between the 0 and 8 yr soils; however, 8 yr soils featured the presence of cyanobacteria, a division wholly absent from the 0 yr soils. Taken together, our results suggest that microbes are consuming allochtonous organic matter sources in the most recently exposed soils. Once this carbon source is depleted, a competitive advantage may be ceded to microbes not reliant on in situ nutrient sources.
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Soil Biology and Biochemistry.2021; 154: 108127. CrossRef - Contrasting early successional dynamics of bacterial and fungal communities in recently deglaciated soils of the maritime Antarctic
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Molecular Ecology.2021; 30(17): 4231. CrossRef - Topsoil organic matter build‐up in glacier forelands around the world
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Global Change Biology.2021; 27(8): 1662. CrossRef - Living at the Edge: Increasing Stress for Plants 2–13 Years After the Retreat of a Tropical Glacier
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Frontiers in Ecology and Evolution.2021;[Epub] CrossRef - Basal thermal regime affects the biogeochemistry of subglacial systems
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Geomicrobiology Journal.2020; 37(6): 497. CrossRef - The disappearing periglacial ecosystem atop Mt. Kilimanjaro supports both cosmopolitan and endemic microbial communities
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Scientific Reports.2019;[Epub] CrossRef - High nitrogen contribution by Gunnera magellanica and nitrogen transfer by mycorrhizas drive an extraordinarily fast primary succession in sub‐Antarctic Chile
Alberto Benavent‐González, José Raggio, Johana Villagra, José Manuel Blanquer, Ana Pintado, Ricardo Rozzi, T. G. Allan Green, Leopoldo G. Sancho
New Phytologist.2019; 223(2): 661. CrossRef - Soil microbial phosphorus turnover and identity of algae and fungi in biological soil crusts along a transect in a glacier foreland
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FEMS Microbiology Letters.2019;[Epub] CrossRef - Genomic profiling of four cultivated Candidatus Nitrotoga spp. predicts broad metabolic potential and environmental distribution
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The ISME Journal.2018; 12(12): 2864. CrossRef - Metagenomic insights into diazotrophic communities across Arctic glacier forefields
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FEMS Microbiology Ecology.2018;[Epub] CrossRef - Distribution of soil nitrogen and nitrogenase activity in the forefield of a High Arctic receding glacier
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Annals of Glaciology.2018; 59(77): 87. CrossRef - Time lag between glacial retreat and upward migration alters tropical alpine communities
Anaïs Zimmer, Rosa I. Meneses, Antoine Rabatel, Alvaro Soruco, Olivier Dangles, Fabien Anthelme
Perspectives in Plant Ecology, Evolution and Systematics.2018; 30: 89. CrossRef - Interspecific Plant Interactions Reflected in Soil Bacterial Community Structure and Nitrogen Cycling in Primary Succession
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Oecologia.2017; 185(3): 513. CrossRef - Biogeochemical Stoichiometry Reveals P and N Limitation Across the Post-glacial Landscape of Denali National Park, Alaska
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Journal of Ecology.2016; 104(6): 1555. CrossRef - Relative Abundance of Nitrotoga spp. in a Biofilter of a Cold-Freshwater Aquaculture Plant Appears To Be Stimulated by Slightly Acidic pH
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Applied and Environmental Microbiology.2016; 82(6): 1838. CrossRef - Changes in the fungal community of Austre Brøggerbreen deglaciation area, Ny-Ålesund, Svalbard, High Arctic
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Mycoscience.2016; 57(6): 448. CrossRef -
Nitrotoga-like bacteria are previously unrecognized key nitrite oxidizers in full-scale wastewater treatment plants
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Polar Biology.2015; 38(5): 689. CrossRef - Pyrosequencing-Based Assessment of the Microbial Community Structure of Pastoruri Glacier Area (Huascarán National Park, Perú), a Natural Extreme Acidic Environment
Elena González-Toril, Esther Santofimia, Yolanda Blanco, Enrique López-Pamo, Manuel J. Gómez, Miguel Bobadilla, Rolando Cruz, Edwin Julio Palomino, Ángeles Aguilera
Microbial Ecology.2015; 70(4): 936. CrossRef - Multiple adaptations to polar and alpine environments within cyanobacteria: a phylogenomic and Bayesian approach
Nathan A. M. Chrismas, Alexandre M. Anesio, Patricia Sánchez-Baracaldo
Frontiers in Microbiology.2015;[Epub] CrossRef - SHIMMER (1.0): a novel mathematical model for microbial and biogeochemical dynamics in glacier forefield ecosystems
J. A. Bradley, A. M. Anesio, J. S. Singarayer, M. R. Heath, S. Arndt
Geoscientific Model Development.2015; 8(10): 3441. CrossRef - Patterns of organic acids exuded by pioneering fungi from a glacier forefield are affected by carbohydrate sources
Ivano Brunner, Asena Goren, Alessandro Schlumpf
Environmental Research Letters.2014; 9(2): 025002. CrossRef - The nitrogen cycle in cryoconites: naturally occurring nitrification‐denitrification granules on a glacier
Takahiro Segawa, Satoshi Ishii, Nobuhito Ohte, Ayumi Akiyoshi, Akinori Yamada, Fumito Maruyama, Zhongqin Li, Yuichi Hongoh, Nozomu Takeuchi
Environmental Microbiology.2014; 16(10): 3250. CrossRef - Do bacterial and fungal communities assemble differently during primary succession?
S. K. Schmidt, D. R. Nemergut, J. L. Darcy, R. Lynch
Molecular Ecology.2014; 23(2): 254. CrossRef - Microbial community dynamics in the forefield of glaciers
James A. Bradley, Joy S. Singarayer, Alexandre M. Anesio
Proceedings of the Royal Society B: Biological Sciences.2014; 281(1795): 20140882. CrossRef - Diversity, resistance and resilience of the bacterial communities at two alpine glacier forefields after a reciprocal soil transplantation
Marco Meola, Anna Lazzaro, Josef Zeyer
Environmental Microbiology.2014; 16(6): 1918. CrossRef - Identification of biomass utilizing bacteria in a carbon‐depleted glacier forefield soil by the use of 13C DNA stable isotope probing
Anita Zumsteg, Stefan Schmutz, Beat Frey
Environmental Microbiology Reports.2013; 5(3): 424. CrossRef - Culturable bacteria community development in postglacial soils of Ecology Glacier, King George Island, Antarctica
Marek K. Zdanowski, Magdalena J. Żmuda-Baranowska, Piotr Borsuk, Aleksander Świątecki, Dorota Górniak, Dorota Wolicka, Katarzyna M. Jankowska, Jakub Grzesiak
Polar Biology.2013; 36(4): 511. CrossRef - Bacterial and fungal community responses to reciprocal soil transfer along a temperature and soil moisture gradient in a glacier forefield
Anita Zumsteg, Erland Bååth, Beat Stierli, Josef Zeyer, Beat Frey
Soil Biology and Biochemistry.2013; 61: 121. CrossRef - Molecular characterization of phototrophic microorganisms in the forefield of a receding glacier in the Swiss Alps
Beat Frey, Lukas Bühler, Stefan Schmutz, Anita Zumsteg, Gerhard Furrer
Environmental Research Letters.2013; 8(1): 015033. CrossRef - Actinobacteria—An Ancient Phylum Active in Volcanic Rock Weathering
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Geomicrobiology Journal.2013; 30(8): 706. CrossRef - Bacterial community structure on two alpine debris-covered glaciers and biogeography of Polaromonas phylotypes
Andrea Franzetti, Valeria Tatangelo, Isabella Gandolfi, Valentina Bertolini, Giuseppina Bestetti, Guglielmina Diolaiuti, Carlo D’Agata, Claudia Mihalcea, Claudio Smiraglia, Roberto Ambrosini
The ISME Journal.2013; 7(8): 1483. CrossRef - Changes in assembly processes in soil bacterial communities following a wildfire disturbance
Scott Ferrenberg, Sean P O’Neill, Joseph E Knelman, Bryan Todd, Sam Duggan, Daniel Bradley, Taylor Robinson, Steven K Schmidt, Alan R Townsend, Mark W Williams, Cory C Cleveland, Brett A Melbourne, Lin Jiang, Diana R Nemergut
The ISME Journal.2013; 7(6): 1102. CrossRef - Bacterial community structure and function change in association with colonizer plants during early primary succession in a glacier forefield
Joseph E. Knelman, Teresa M. Legg, Sean P. O’Neill, Christopher L. Washenberger, Antonio González, Cory C. Cleveland, Diana R. Nemergut
Soil Biology and Biochemistry.2012; 46: 172. CrossRef - Characterizing microbial communities through space and time
Antonio Gonzalez, Andrew King, Michael S Robeson II, Sejin Song, Ashley Shade, Jessica L. Metcalf, Rob Knight
Current Opinion in Biotechnology.2012; 23(3): 431. CrossRef - Prokaryotic diversity in sediments beneath two polar glaciers with contrasting organic carbon substrates
Marek Stibal, Fariha Hasan, Jemma L. Wadham, Martin J. Sharp, Alexandre M. Anesio
Extremophiles.2012; 16(2): 255. CrossRef - Soil microbial carbon and nitrogen transformations at a glacial foreland on Anvers Island, Antarctic Peninsula
Sarah L. Strauss, Ferran Garcia-Pichel, Thomas A. Day
Polar Biology.2012; 35(10): 1459. CrossRef - Bacterial Community Structure in the Drinking Water Microbiome Is Governed by Filtration Processes
Ameet J. Pinto, Chuanwu Xi, Lutgarde Raskin
Environmental Science & Technology.2012; 46(16): 8851. CrossRef - Assessment of soil bacterial communities on Alexander Island (in the maritime and continental Antarctic transitional zone)
C. W. Chong, P. Convey, D. A. Pearce, I. K. P. Tan
Polar Biology.2012; 35(3): 387. CrossRef - Bacterial diversity in the foreland of the Tianshan No. 1 glacier, China
Xiukun Wu, Wei Zhang, Guangxiu Liu, Xuan Yang, Ping Hu, Tuo Chen, Gaosen Zhang, Zhongqin Li
Environmental Research Letters.2012; 7(1): 014038. CrossRef - Diverse microbial community from the coalbeds of the Ordos Basin, China
Yue-Qin Tang, Pan Ji, Guo-Li Lai, Chang-Qiao Chi, Ze-Shen Liu, Xiao-Lei Wu
International Journal of Coal Geology.2012; 90-91: 21. CrossRef - Pioneering fungi from the Damma glacier forefield in the Swiss Alps can promote granite weathering
I. BRUNNER, M. PLÖTZE, S. RIEDER, A. ZUMSTEG, G. FURRER, B. FREY
Geobiology.2011; 9(3): 266. CrossRef - Distribution of High Bacterial Taxa Across the Chronosequence of Two Alpine Glacier Forelands
Laurent Philippot, Dagmar Tscherko, David Bru, Ellen Kandeler
Microbial Ecology.2011; 61(2): 303. CrossRef - Global Distribution of Polaromonas Phylotypes - Evidence for a Highly Successful Dispersal Capacity
John L. Darcy, Ryan C. Lynch, Andrew J. King, Michael S. Robeson, Steven K. Schmidt, Purificación López-García
PLoS ONE.2011; 6(8): e23742. CrossRef
Review
- Effects of Elevated Atmospheric CO_2 Concentrations on Soil Microorganisms
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Chris Freeman , Seon-Young Kim , Seung-Hoon Lee , Hojeong Kang
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J. Microbiol. 2004;42(4):267-277.
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DOI: https://doi.org/2111 [pii]
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Abstract
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Effects of elevated CO_2 on soil microorganisms are known to be mediated by various interactions with plants, for which such effects are relatively poorly documented. In this review, we summarize and synthesize results from studies assessing impacts of elevated CO_2 on soil ecosystems, focusing primarily on plants and a variety the of microbial processes. The processes considered include changes in microbial biomass of C and N, microbial number, respiration rates, organic matter decomposition, soil enzyme activities, microbial community composition, and functional groups of bacteria mediating trace gas emission such as methane and nitrous oxide. Elevated CO_2 in atmosphere may enhance certain microbial processes such as CH_4 emission from wetlands due to enhanced carbon supply from plants. However, responses of extracellular enzyme activities and microbial community structure are still controversy, because interferences with other factors such as the types of plants, nutrient availabilitial in soil, soil types, analysis methods, and types of CO_2 fumigation systems are not fully understood.