Acute lung injury caused by Candida albicans could result in
high mortality and morbidity. MicroRNA-155 (miR-155) and
suppressor of cytokine signaling 1 (SOCS1) have been believed
to play a key in the regulation of inflammatory response.
Whether miR-155/SOCS1 axis could regulate the acute lung
injury caused by C. albicans has not been reported. The acute
lung injury animal model was established with acute infection
of C. albicans. miR-155 inhibitor, miR-155 mimic, and
sh-SOCS1 were constructed. The binding site between miR-
155 and SOCS1 was identified with dual luciferase reporter
assay. Knockdown of miR-155 markedly inhibited the germ
tube formation of C. albicans. Knockdown of miR-155 significantly
up-regulated the expression of SOCS1, and the binding
site between miR-155 and SOCS1 was identified. Knockdown
of miR-155 improved the acute lung injury, suppressed
inflammatory factors and fungus loading through SOCS1.
Knockdown of SOCS1 greatly reversed the influence of miR-
155 inhibitor on the cell apoptosis in vitro. The improvement
of acute lung injury caused by C. albicans, suppression of inflammatory
response and C. albicans infection, and inhibitor
of cell apoptosis were achieved by knocking down miR-155
through SOCS1. This research might provide a new thought
for the prevention and treatment of acute lung injury caused
by C. albicans through targeting miR-155/SOCS1 axis.
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The increasing temperature in Arctic tundra deepens the
active layer, which is the upper layer of permafrost soil that
experiences repeated thawing and freezing. The increasing
of soil temperature and the deepening of active layer seem
to affect soil microbial communities. Therefore, information
on soil microbial communities at various soil depths is essential
to understand their potential responses to climate change
in the active layer soil. We investigated the community structure
of soil bacteria in the active layer from moist acidic tundra
in Council, Alaska. We also interpreted their relationship
with some relevant soil physicochemical characteristics along
soil depth with a fine scale (5 cm depth interval). The bacterial
community structure was found to change along soil
depth. The relative abundances of Acidobacteria, Gammaproteobacteria,
Planctomycetes, and candidate phylum WPS-2
rapidly decreased with soil depth, while those of Bacteroidetes,
Chloroflexi, Gemmatimonadetes, and candidate AD3 rapidly
increased. A structural shift was also found in the soil bacterial
communities around 20 cm depth, where two organic
(upper Oi and lower Oa) horizons are subdivided. The quality
and the decomposition degree of organic matter might
have influenced the bacterial community structure. Besides
the organic matter quality, the vertical distribution of bacterial
communities was also found to be related to soil pH and
total phosphorus content. This study showed the vertical
change of bacterial community in the active layer with a fine
scale resolution and the possible influence of the quality of soil
organic matter on shaping bacterial community structure.
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