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- Optimal Protocol for Enumeration of Attached Bacteria on Glass Slides
- Hyun Sang Lee , Kae Kyoung Kwon , Jong Ho Lee , Hong Kum Lee
- J. Microbiol. 1999;37(4):263-266.
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Abstract
- In examining bacterial growth on glass surfaces immersed in sea water, we found serious differences between enumeration methods. Therefore, we compared various methods and found sonication and direct count methods were superior to other methods. Since the direct count method was not suitable for long-term investigation, we chose the sonication method and confirmed that sonication periods 8 times for 30 seconds was optimal for the detachment of bacteria from glass surfaces.
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