Journal of Microbiology

Journal Article

Characterization of antibiotic-resistant, coagulase-negative staphylococci from fresh produce and description of Staphylococcus shinii sp. nov. isolated from chives: Gyu-Sung Cho , Bo Li , Erik Brinks , Charles , M.A.P. Franz; J. Microbiol. 2022;60(9):877-889. Published online June 22, 2022; DOI: https://doi.org/10.1007/s12275-022-2100-5

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Coagulase-negative Staphylococcus (CoNS) species may possess antibiotic resistance genes and have been associated with nosocomial infections. In this study, 91 CoNS with decreased susceptibility to oxacillin were isolated from fresh produce using oxacillin containing agar plates. Their antibiotic resistances were determined phenotypically and all isolates were identified by rep-PCR, 16S rRNA and rpoB gene sequencing. Furthermore, the genomes of representative strains were sequenced in order to confirm species identification by phylogenomics. The majority (64 of 91) of the CoNS strains could be identified as Mammaliicoccus (M.) fleurettii, while 13 were identified as M. sciuri, 8 as M. vitulinus, 2 as Staphylococcus (S.) epidermidis and single strains each as S. warneri, S. xylosus, Staphylococcus spp. and S. casei. Most of the strains were generally susceptible to clinically-relevant antibiotics, but only few (< 7%) strains possessed multiple resistances. Both oxacillin and cefoxitin resistant isolates were considered to be presumptive methicillin-resistant CoNS. From whole genome sequencing data of 6 representative strains, the mecA gene, accessory genes and the SCC loci were compared, which revealed high variability between some of the strains. The major fatty acids of K22-5MT strain included anteiso-C15:0, iso-C15:0, iso-C17:0, anteiso-C17:0, C18:0, and C20:0. Average nucleotide identity and digital DNA-DNA hybridization values indicated that Staphylococcus strain K22-5MT was below the species delineation cutoff values for ANI (less than 91%) and DDH (less than 44.4%), with the most closely related species being the S. pseudoxylosus S04009T type strain. Thus, strain K22- 5MT (=DSM 112532T, =LMG 32324T) represents a novel species, for which the name Staphylococcus shinii sp. nov. is proposed.

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Scandinavium lactucae sp. nov. Isolated from Healthy Lettuce in South Korea
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Morphological changes in human gastric epithelial cells induced by nuclear targeting of Helicobacter pylori urease subunit A: Jung Hwa Lee , So Hyun Jun , Jung-Min Kim , Seung Chul Baik , Je Chul Lee; J. Microbiol. 2015;53(6):406-414. Published online May 30, 2015; DOI: https://doi.org/10.1007/s12275-015-5085-5

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Abstract

Nuclear targeting of bacterial proteins and their pathological effects on host cells are an emerging pathogenic mechanism in bacteria. We have previously reported that urease subunit A (UreA) of Helicobacter pylori targets the nuclei of COS-7 cells through nuclear localization signals (NLSs). This study further investigated whether UreA of H. pylori targets the nuclei of gastric epithelial cells and then induces molecular and cellular changes in the host cells. H. pylori 26695 strain produced and secreted outer membrane vesicles (OMVs). UreA was translocated into gastric epithelial AGS cells through outer membrane vesicles (OMVs) and then targeted the nuclei of AGS cells. Nuclear targeting of rUreA did not induce host cell death, but resulted in morphological changes, such as cellular elongation, in AGS cells. In contrast, AGS cells treated with rUreAΔNLS proteins did not show this morphological change. Next generation sequencing revealed that nuclear targeting of UreA differentially regulated 102 morphogenesis- related genes, of which 67 and 35 were up-regulated and down-regulated, respectively. Our results suggest that nuclear targeting of H. pylori UreA induces both molecular and cellular changes in gastric epithelial cells.

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Effects of Exosomes Derived From Helicobacter pylori Outer Membrane Vesicle-Infected Hepatocytes on Hepatic Stellate Cell Activation and Liver Fibrosis Induction
Masoumeh Ebadi Zahmatkesh, Mariyeh Jahanbakhsh, Negin Hoseini, Saina Shegefti, Amir Peymani, Hossein Dabin, Rasoul Samimi, Shahin Bolori
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Significance of Helicobacter pylori and Its Serological Typing in Gastric Cancer
碧玉张
Advances in Clinical Medicine.2022; 12(12): 11694. CrossRef
Rational Development of Bacterial Ureases Inhibitors
Saurabh Loharch, Łukasz Berlicki
The Chemical Record.2022;[Epub] CrossRef
Emerging therapeutic targets for gastric cancer from a host-Helicobacter pylori interaction perspective
Esmat Abdi, Saeid Latifi-Navid, Fatemeh Abedi Sarvestani, Mohammad Hassan Esmailnejad
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Non-enzymatic properties of Proteus mirabilis urease subunits
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Nuclear trafficking of bacterial effector proteins
Lena Hoang My Le, Le Ying, Richard L. Ferrero
Cellular Microbiology.2021;[Epub] CrossRef
Proteus mirabilis Urease: Unsuspected Non-Enzymatic Properties Relevant to Pathogenicity
Matheus V. C. Grahl, Augusto F. Uberti, Valquiria Broll, Paula Bacaicoa-Caruso, Evelin F. Meirelles, Celia R. Carlini
International Journal of Molecular Sciences.2021; 22(13): 7205. CrossRef
Helicobacter pylori Outer Membrane Vesicles and Extracellular Vesicles from Helicobacter pylori-Infected Cells in Gastric Disease Development
María Fernanda González, Paula Díaz, Alejandra Sandoval-Bórquez, Daniela Herrera, Andrew F. G. Quest
International Journal of Molecular Sciences.2021; 22(9): 4823. CrossRef
Tracking the cargo of extracellular symbionts into host tissues with correlated electron microscopy and nanoscale secondary ion mass spectrometry imaging
Stephanie K. Cohen, Marie‐Stéphanie Aschtgen, Jonathan B. Lynch, Sabrina Koehler, Fangmin Chen, Stéphane Escrig, Jean Daraspe, Edward G. Ruby, Anders Meibom, Margaret McFall‐Ngai
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Role of Probiotics in Prophylaxis of Helicobacter pylori Infection
Kashyapi Chakravarty, Smriti Gaur
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Helicobacter pylori: molecular basis for colonization and survival in gastric environment and resistance to antibiotics. A short review
Sharmila Fagoonee, Rinaldo Pellicano
Infectious Diseases.2019; 51(6): 399. CrossRef
Cross‐Reactivity of Polyclonal Antibodies against Canavalia ensiformis (Jack Bean) Urease and Helicobacter pylori Urease Subunit A Fragments
Zbigniew Jerzy Kaminski, Inga Relich, Iwona Konieczna, Wieslaw Kaca, Beata Kolesinska
Chemistry & Biodiversity.2018;[Epub] CrossRef
Ureases: Historical aspects, catalytic, and non-catalytic properties – A review
Karine Kappaun, Angela Regina Piovesan, Celia Regina Carlini, Rodrigo Ligabue-Braun
Journal of Advanced Research.2018; 13: 3. CrossRef
The Impact of Helicobacter pylori Urease upon Platelets and Consequent Contributions to Inflammation
Adriele Scopel-Guerra, Deiber Olivera-Severo, Fernanda Staniscuaski, Augusto F. Uberti, Natália Callai-Silva, Natália Jaeger, Bárbara N. Porto, Celia R. Carlini
Frontiers in Microbiology.2017;[Epub] CrossRef
A New Role for Helicobacter pylori Urease: Contributions to Angiogenesis
Deiber Olivera-Severo, Augusto F. Uberti, Miguel S. Marques, Marta T. Pinto, Maria Gomez-Lazaro, Céu Figueiredo, Marina Leite, Célia R. Carlini
Frontiers in Microbiology.2017;[Epub] CrossRef

Effect of Salmonella Treatment on an Implanted Tumor (CT26) in a Mouse Model: Misun Yun , SangO Pan , Sheng- Nan Jiang , Vu Hong Nguyen , Seung-Hwan Park , Che-Hun Jung , Hyung-Seok Kim , Jung-Joon Min , Hyon E. Choy , Yeongjin Hong; J. Microbiol. 2012;50(3):502-510. Published online June 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2090-9

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Abstract: The use of bacteria has contributed to recent advances in targeted cancer therapy especially for its tumor-specific accumulation and proliferation. In this study, we investigated the molecular events following bacterial therapy using an attenuated Salmonella Typhimurium defective in ppGpp synthesis (ΔppGpp), by analyzing those proteins differentially expressed in tumor tissues from treated and untreated mice. CT26 murine colon cancer cells were implanted in BALB/c mice and allowed to form tumors. The tumor-bearing mice were treated with the attenuated Salmonella Typhimurium. Tumor tissues were analyzed by 2D-PAGE. Fourteen differentially expressed proteins were identified by mass spectrometry. The analysis revealed that cytoskeletal components, including vimentin, drebrin-like protein, and tropomyosinalpha 3, were decreased while serum proteins related to heme or iron metabolism, including transferrin, hemopexin, and haptoglobin were increased. Subsequent studies revealed that the decrease in cytoskeletal components occurred at the transcriptional level and that the increase in heme and iron metabolism proteins occurred in liver. Most interestingly, the same pattern of increased expression of transferrin, hemopexin, and haptoglobin was observed following radiotherapy at the dosage of 14 Gy.

Differential Expression of citA Gene Encoding the Mitochondrial Citrate Synthase of Aspergillus nidulans in Response to Developmental Status and Carbon Sources: In Sook Min , Ji Young Bang , Soon Won Seo , Cheong Ho Lee , Pil Jae Maeng; J. Microbiol. 2010;48(2):188-198. Published online May 1, 2010; DOI: https://doi.org/10.1007/s12275-010-0096-8

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Abstract: As an extension of our previous studies on the mitochondrial citrate synthase of Aspergillus nidulans and cloning of its coding gene (citA), we analyzed differential expression of citA in response to the progress of development and change of carbon source. The cDNA consisted of 1,700 nucleotides and was predicted to encode a 474-amino acid protein. By comparing the cDNA sequence with the corresponding genomic sequence, we confirmed that citA gene contains 7 introns and that its transcription starts at position -26 (26-nucleotide upstream from the initiation codon). Four putative CreA binding motifs and three putative stress-response elements (STREs) were found within the 1.45-kb citA promoter region. The mode of citA expression was examined by both Northern blot and confocal microscopy using green fluorescent protein (sGFP) as a vital reporter. During vegetative growth and asexual development, the expression of citA was ubiqiutous throughout the whole fungal body including mycelia and conidiophores. During sexual development, the expression of citA was quite strong in cleistothecial shells, but significantly weak in the content of cleistothecia including ascospores. Acetate showed a strong inductive effect on citA expression, which is subjected to carbon catabolite repression (CCR) caused by glucose. The recombinant fusion protein CitA40::sGFP (sGFP containing the 40-amino acid N-terminal segment of CitA) was localized into mitochondria, which supports that a mitochondrial targeting signal is included within the 40-amino acid N-terminal segment of CitA.

Enhancement of Gene Delivery to Cancer Cells by a Retargeted Adenovirus: Kwang Seok Oh , Jeffrey A. Engler , Insil Joung; J. Microbiol. 2005;43(2):179-182.; DOI: https://doi.org/2164 [pii]

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Abstract: The inefficiency of in vivo gene transfer using currently available vectors reflects a major hurdle in cancer gene therapy. Both viral and non-viral approaches that improve gene transfer efficiency have been described, but suffer from a number of limitations. Herein, a fiber-modified adenovirus, carrying the small peptide ligand on the capsid, was tested for the delivery of a transgene to cancer cells. The fiber-modified adenovirus was able to mediate the entry and expression of a [beta]-galactosidase into cancer cells with increased efficiency compared to the unmodified adenovirus. Particularly, the gene transfer efficiency was improved up to 5 times in OVCAR3 cells, an ovarian cancer cell line. Such transduction systems hold promise for delivering genes to transferrin receptor overexpressing cancer cells, and could be used for future cancer gene therapy.

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