Journal of Microbiology

Journal Article

Sulforaphane kills Mycobacterium tuberculosis H37Ra and Mycobacterium smegmatis mc2155 through a reactive oxygen species dependent mechanism: Yongjie Zhao , Shengwen Shang , Ya Song , Tianyue Li , Mingliang Han , Yuexuan Qin , Meili Wei , Jun Xi , Bikui Tang; J. Microbiol. 2022;60(11):1095-1105. Published online September 1, 2022; DOI: https://doi.org/10.1007/s12275-022-2284-8

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Mycobacterium tuberculosis (M. tuberculosis) is a highly pathogenic intracellular pathogen that causes tuberculosis (TB), the leading cause of mortality from single infections. Redox homeostasis plays a very important role in the resistance of M. tuberculosis to antibiotic damage and various environmental stresses. The antioxidant sulforaphane (SFN) has been reported to exhibit anticancer activity and inhibit the growth of a variety of bacteria and fungi. Nonetheless, it remains unclear whether SFN exhibits anti-mycobacterial activity. Our
results
showed that the SFN against M. tuberculosis H37Ra exhibited bactericidal activity in a time and dose-dependent manner. The anti-tubercular activity of SFN was significantly correlated with bacterial reactive oxygen species (ROS) levels. In addition, SFN promoted the bactericidal effect of macrophages on intracellular bacteria in a dose-dependent manner, mediated by increasing intracellular mitochondrial ROS levels and decreasing cytoplasmic ROS levels. Taken together, our data revealed the previously unrecognized antimicrobial functions of SFN. Future studies focusing on the mechanism of SFN in macrophages against M. tuberculosis are essential for developing new host-directed therapeutic approaches against TB.

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NK-derived exosome miR-1249-3p inhibits Mycobacterium tuberculosis survival in macrophages by targeting SKOR1
Fengqian Ma, Xuan Wang, Zhanghua Qiu, Shoupeng Ding, Wenya Du, Yumei Dai, Tao Ma, Linzhi Yue, Guofu Wang, Tao Wang, Ling Geng, Lixian Wu
Cytokine.2024; 175: 156481. CrossRef
Lactobacillus plantarum increase the sulforaphane formation efficiency via microbial-targeted delivery system in vivo
Yunping Wang, Yiteng Zhang, Xiude Li, Liping Luo, Fangjian Ning, Tao Liu, Jinwang Li
Food Bioscience.2024; 62: 105544. CrossRef
Identifying autophagy-related genes as potential targets for immunotherapy in tuberculosis
Sifang Xiao, Ting Zhou, Jianhua Pan, Xiaohua Ma, Guomin Shi, Binyuan Jiang, Yan-gen Xiang
International Immunopharmacology.2023; 118: 109956. CrossRef

Research Support, Non-U.S. Gov'ts

In Vitro Development and Transfer of Resistance to Chlortetracycline in Bacillus subtilis: Menghong Dai , Junjie Lu , Yulian Wang , Zhenli Liu , Zonghui Yuan; J. Microbiol. 2012;50(5):807-812. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-1454-5

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Abstract: The present criteria and rules controlling the approval of the use of probiotics are limited to antibiotic resistance patterns and the presence of antibiotic resistance genes in bacteria. There is little information available in the literature regarding the risk of the usage of probiotics in the presence of antibiotic pressure. In this study we investigated the development and transfer of antibiotic resistance in Bacillus subtilis selected in vitro by chlortetracycline in a stepwise manner. Bacillus subtilis was exposed to increasing concentrations of chlortetracyclineto induce in vitro resistance to chlortetracycline, and the minimal inhibitory concentrations were determinedfor the mutants. Resistant B. subtilis were conjugated with Escherichia coli NK5449 and Enterococcus faecalis JH2-2 using the filter mating. Three B. subtilis tetracycline resistant mutants (namely, BS-1, BS-2, and BS-3) were derived in vitro. A tetracycline resistant gene, tet (K), was found in the plasmids of BS-1 and BS-2. Three conjugates (BS-1N, BS-2N, and BS-3N) were obtained when the resistant B. subtilis was conjugated with E. coli NK5449. The conjugation frequencies for the BS-1N, BS-2N, and BS-3N conjugates were 4.57×10-7, 1.4×10-7, and 1.3×10-8, respectively. The tet(K) gene was found only in the plasmids of BS-1N. These results indicate that long-term use of probiotics under antibiotic selection pressure could cause antibiotic resistance, and the resistance gene could be transferred to other bacteria. The risk arising from the use of probiotics under antibiotic pressure should be considered in the criteria and rules for the safety assessment of probiotics.

Prevalence of Tetracycline Resistance Genes in Greek Seawater Habitats: Theodora L. Nikolakopoulou , Eleni P. Giannoutsou , Adamandia A. Karabatsou , Amalia D. Karagouni; J. Microbiol. 2008;46(6):633-640. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0080-8

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The presence of selected tetracycline resistance (TcR) genes was studied in different Greek seawater habitats, originated from wastewater treatment facilities, fishfarm, and coastal environments. The methods employed included assessment of the presence of twelve gene clusters by PCR, followed by hybridization with specific probes, in habitat extracted DNA, TcR bacteria, and exogenous isolated plasmids conferring TcR. The direct DNA-based analysis showed that tet(Α) and tet(K) genes were detected in all habitats, whilst tet(C) and tet(E) were present in fishfarm and wastewater effluent samples and tet(M) was detected in fishfarm and coastal samples. Resistance genes tet(A), tet(C), tet(K), and tet(M) were detected in 60 of the 89 isolates screened. These isolates were identified by fatty acid methyl ester analysis (FAME) as Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Staphylococcus strains. The presence of the TcR genes in 15% of the bacterial isolates coincided with the presence of IncP plasmids. A habitat-specific dissemination of IncP alpha plasmids in wastewater effluent isolates and of IncP beta plasmids in fishfarm isolates was observed. Exogenous isolation demonstrated the presence of plasmids harbouring TcR genes in all the habitats tested. Plasmids were shown to carry tet(A), tet(C), tet(E), and tet(K) genes. It is concluded that TcR genes are widespread in the seawater habitats studied and often occur on broad host range plasmids that seem to be well disseminated in the bacterial communities.

Citations

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Ilda Czobor Barbu, Irina Gheorghe-Barbu, Georgiana Alexandra Grigore, Corneliu Ovidiu Vrancianu, Mariana Carmen Chifiriuc
International Journal of Molecular Sciences.2023; 24(9): 7892. CrossRef
Isolation and characterization of a novel Tenacibaculum species and a corresponding bacteriophage from a Mediterranean fish hatchery: Description of Tenacibaculum larymnensis sp. nov. and Tenacibaculum phage Larrie
Maria Ioanna Tsertou, Adriana Triga, Stavros Droubogiannis, Constantina Kokkari, Grammatiki Anasi, Pantelis Katharios
Frontiers in Microbiology.2023;[Epub] CrossRef
Composition and distribution of bacterial communities and antibiotic resistance genes in fish of four mariculture systems
Mingqing Zhang, Liping Hou, Yating Zhu, Cuiping Zhang, Wen Li, Xingxing Lai, Jinlin Yang, Shuisheng Li, Hu Shu
Environmental Pollution.2022; 311: 119934. CrossRef
Antibiotic-Resistant Bacteria in Aquaculture and Climate Change: A Challenge for Health in the Mediterranean Area
Milva Pepi, Silvano Focardi
International Journal of Environmental Research and Public Health.2021; 18(11): 5723. CrossRef
Acinetobacter baumannii Antibiotic Resistance Mechanisms
Ioannis Kyriakidis, Eleni Vasileiou, Zoi Dorothea Pana, Athanasios Tragiannidis
Pathogens.2021; 10(3): 373. CrossRef
Antibiotic-Resistant Bacteria in Greywater and Greywater-Irrigated Soils
Eleonora Troiano, Luciano Beneduce, Amit Gross, Zeev Ronen
Frontiers in Microbiology.2018;[Epub] CrossRef
Interplay of a non-conjugative integrative element and a conjugative plasmid in the spread of antibiotic resistance via suicidal plasmid transfer from an aquaculture Vibrio isolate
Lisa Nonaka, Tatsuya Yamamoto, Fumito Maruyama, Yuu Hirose, Yuki Onishi, Takeshi Kobayashi, Satoru Suzuki, Nobuhiko Nomura, Michiaki Masuda, Hirokazu Yano, Axel Cloeckaert
PLOS ONE.2018; 13(6): e0198613. CrossRef
Multiple Antimicrobial Resistance and Novel Point Mutation in Fluoroquinolone-ResistantEscherichia coliIsolates from Mangalore, India
Kogaluru Shivakumaraswamy Santhosh, Vijaya Kumar Deekshit, Moleyuru Nagarajappa Venugopal, Iddya Karunasagar, Indrani Karunasagar
Microbial Drug Resistance.2017; 23(8): 994. CrossRef
Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment
Monika Harnisz, Ewa Korzeniewska, Sebastian Niestępski, Adriana Osińska, Beata Nalepa, B. Kaźmierczak, M. Kutyłowska, K. Piekarska, H. Jouhara, J. Danielewicz
E3S Web of Conferences.2017; 22: 00063. CrossRef
Exogenous isolation of conjugative plasmids from pesticide contaminated soil
Reshma Anjum, Elisabeth Grohmann, Abdul Malik
World Journal of Microbiology and Biotechnology.2012; 28(2): 567. CrossRef
Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2012 update)

EFSA Journal.2012;[Epub] CrossRef
Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

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Tetracycline Resistance-Encoding Plasmid from Bacillus sp. Strain #24, Isolated from the Marine Sponge Haliclona simulans
Robert W. Phelan, Charles Clarke, John P. Morrissey, Alan D. W. Dobson, Fergal O'Gara, Teresa M. Barbosa
Applied and Environmental Microbiology.2011; 77(1): 327. CrossRef
Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2010 update)

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The pattern, association with multidrug-resistance and transferability of plasmid-mediated tetracycline resistance inEscherichia coli isolates from the poultry in Iran
Tahereh Falsafi, Monir Ebrahimi, Ezat Asgarani, Vahid Mirtorabi
Annals of Microbiology.2009;[Epub] CrossRef

Molecular Characterization of Antibiotic Resistant Escherichia coli Strains Isolated from Tap and Spring Waters in a Coastal Region in Turkey: Osman Birol Ozgumus , Elif Celik-Sevim , Sengul Alpay-Karaoglu , Cemal Sandalli , Ali Sevim; J. Microbiol. 2007;45(5):379-387.; DOI: https://doi.org/2600 [pii]

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Abstract PDF: A hundred and seventeen antibiotic-resistant Escherichia coli strains were isolated from public tap and spring waters which were polluted by fecal coliforms. There were no significant differences between two water sources as to the coliform pollution level (p> 0.05). All E. coli isolates were detected to be resistant to one or more antibiotics tested. Nearly 42% of the isolates showed multiresistant phenotype. Three (2.5%) of these isolates contained class 1 integron. Sequencing analysis of variable regions of the class 1 integrons showed two gene cassette arrays, dfr1-aadA1 and dhfrA17-aadA5. Resistance to ampicillin, tetracycline or trimethoprim-sulfamethoxazole was transferable according to the results of conjugation experiments. The rate of tetracycline resistance was 15%. tet(A)-mediated tetracycline resistance was widespread among tetracycline-resistant E. coli isolates. Genotyping by BOX-polymerase chain reaction (BOX-PCR) showed that some of the strains were epidemiologically related. This is the first report on the prevalence and characterization of class 1 integron-containing E. coli isolates of environmental origin in Turkey.

The Diversity of Multi-drug Resistance Profiles in Tetracycline-Resistant Vibrio Species Isolated from Coastal Sediments and Seawater: Farzana Ashrafi Neela , Lisa Nonaka , Satoru Suzuki; J. Microbiol. 2007;45(1):64-68.; DOI: https://doi.org/2489 [pii]

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Abstract PDF: In this study we examined the multi-drug resistance profiles of the tetracycline (TC) resistant genus Vibrio to determine its susceptibility to two β-lactams, ampicillin (ABPC), and mecillinam (MPC), as well as to macrolide, erythromycin (EM). The results showed various patterns of resistance among strains that were isolated from very close geographical areas during the same year, suggesting diverse patterns of drug resistance in environmental bacteria from this area. In addition, the cross-resistance patterns suggested that the resistance determinants among Vibrio spp. are acquired differently within the sediment and seawater environments.

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