MSK
Search
- Page Path
- HOME > Search
Journal Article
- Characterization of Marinilongibacter aquaticus gen. nov., sp. nov., a unique marine bacterium harboring four CRISPR-Cas systems in the phylum Bacteroidota
- Dao-Feng Zhang , Yu-Fang Yao , Hua-Peng Xue , Zi-Yue Fu , Xiao-Mei Zhang , Zongze Shao
- J. Microbiol. 2022;60(9):905-915. Published online August 1, 2022
- DOI: https://doi.org/10.1007/s12275-022-2102-3
- 60 View
- 0 Download
- 5 Web of Science
- 6 Crossref
-
Abstract
- A novel bacterium, designated YYF0007T, was isolated from an agar-degrading co-culture. The strain was found harboring four CRISPR-Cas systems of two classes in the chromosome and subsequently subjected to a study on polyphasic taxonomy. Pairwise analyses of the 16S rRNA gene sequences indicated that strain YYF0007T had highest 16S rRNA gene sequence similarity (92.2%) to Jiulongibacter sediminis JN- 14-9T. The phylogenomic trees based on the 16S rRNA gene and 269 single-copy orthologous gene clusters (OCs) indicated that strain YYF0007T should be recognized as a novel genus of the family Spirosomaceae. The cells were Gramstain- negative, nonmotile, strictly aerobic, and straight long rods with no flagellum. Optimum growth occurred at 28°C and pH 7.0 with the presence of NaCl concentration 1.0–3.0% (w/v). The strain showed oxidase and catalase activities. The major fatty acids were C16:1ω5c, iso-C15:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The predominant isoprenoid quinone was MK-7. The complete genome size was 4.64 Mb with a DNA G + C content of 44.4%. Further typing of CRISPR-Cas systems in the family Spirosomaceae and the phylum Bacteroidota indicated that it was remarkable for strain YYF0007T featured by such a set of CRISPR-Cas systems. This trait highlights the applications of strain YYF- 0007T in studies on the evolutionary dynamics and bacterial autoimmunity of CRISPR-Cas system as a potential model. The name Marinilongibacter aquaticus gen. nov., sp. nov. is proposed, and the type strain is YYF0007T (= MCCC 1K06017T = GDMCC 1.2428T = JCM 34683T).
-
Citations
Citations to this article as recorded by
- Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects
Jiashun Li, Shuaishuai Wu, Kaidian Zhang, Xueqiong Sun, Wenwen Lin, Cong Wang, Senjie Lin
Microorganisms.2024; 12(1): 118. CrossRef - Unraveling the mechanisms behind sodium persulphate-induced changes in petroleum-contaminated aquifers’ biogeochemical parameters and microbial communities
Yuqi Qi, Jun Zeng, Junshi Tao, Rentao Liu, Renchuan Fu, Chao Yan, Xiao Liu, Na Liu, Yanru Hao
Chemosphere.2024; 351: 141174. CrossRef -
Arcicella gelida sp. nov. and Arcicella lustrica sp. nov., isolated from streams in China and re-examining the taxonomic status of all the genera within the families Spirosomataceae and Cytophagaceae
Huibin Lu, Li Chen, Linpei Huang
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef - Thalassospira aquimaris sp. nov. and Winogradskyella marincola sp. nov. two marine bacteria isolated from an agar-degrading co-culture
Zi-Yue Fu, Dao-Feng Zhang, Meng-Han Huang, Hong-Chuan Wang, Xiao-Ye Chen, Yu-Fang Yao, Yang Yuan, Wen-Jun Li
Antonie van Leeuwenhoek.2024;[Epub] CrossRef - Validation List no. 209. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef - Telluribacter roseus sp. nov., Isolated from the Kumtag Desert Soil
Chu-Ying Feng, Jia-Rui Han, Chun-Yan Lu, Li Gu, Shuai Li, Wen-Hui Lian, Lei Dong, Wen-Jun Li
Current Microbiology.2023;[Epub] CrossRef
- Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects
First
Prev
TOP
