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- Meiotic prophase roles of Pds5 in recombination and chromosome condensation in budding yeast
- Jeong Hwan Joo , Hyun Ah Kang , Keun Pil Kim , Soogil Hong
- J. Microbiol. 2022;60(2):177-186. Published online February 1, 2022
- DOI: https://doi.org/10.1007/s12275-022-1635-9
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- 3 Web of Science
- 3 Crossref
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Abstract
-
Genetic variation in eukaryotes is mediated during meiosis by
the exchange of genetic material between homologous chromosomes
to produce recombinant chromosomes. Cohesin is
essential to promote proper chromosome segregation, chromosome
morphogenesis, and recombination in meiotic cells.
Cohesin consists of three main subunits–Smc1, Smc3, and the
kleisin subunit Mcd1/Scc1 (Rec8 in meiosis)–and cohesin accessory
factors. In Saccharomyces cerevisiae, the cohesin regulatory
subunit Pds5 plays a role in homolog pairing, meiotic
axis formation, and interhomolog recombination. In this
study, we examine the prophase functions of Pds5 by performing
physical analysis of recombination and three-dimensional
high-resolution microscopy analysis to identify its roles in
meiosis-specific recombination and chromosome morphogenesis.
To investigate whether Pds5 plays a role in mitoticlike
recombination, we inhibited Mek1 kinase activity, which
result
ed in switching to sister template bias by Rad51-dependent recombination. Reductions in double-strand breaks and crossover products and defective interhomolog recombination occurred in the absence of Pds5. Furthermore, recombination intermediates, including single-end invasion and double-Holliday junction, were reduced in the absence of Pds5 with Mek1 kinase inactivation compared to Mek1 kinase inactivation cells. Interestingly, the absence of Pds5
result
ed in increasing numbers of chromosomes with hypercompaction of the chromosome axis. Thus, we suggest that Pds5 plays an essential role in recombination by suppressing the pairing of sister chromatids and abnormal compaction of the chromosome axis. -
Citations
Citations to this article as recorded by
- RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination
Jeong H Joo, Soogil Hong, Mika T Higashide, Eui-Hwan Choi, Seobin Yoon, Min-Su Lee, Hyun Ah Kang, Akira Shinohara, Nancy Kleckner, Keun P Kim
Nucleic Acids Research.2024; 52(7): 3794. CrossRef - Cohesin is required for meiotic spindle assembly independent of its role in cohesion in C. elegans
Karen P. McNally, Elizabeth A. Beath, Brennan M. Danlasky, Consuelo Barroso, Ting Gong, Wenzhe Li, Enrique Martinez-Perez, Francis J. McNally, Sarit Smolikove
PLOS Genetics.2022; 18(10): e1010136. CrossRef - Yeast polyubiquitin unit regulates synaptonemal complex formation and recombination during meiosis
Min-Kyung Jo, Kiwon Rhee, Keun Pil Kim, Soogil Hong
Journal of Microbiology.2022; 60(7): 705. CrossRef
- RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination
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