Journal of Microbiology

Journal Article

The Observation of PlcA Mutation and Localization in Aspergillus nidulans: Chun-Seob Ahn , Young Taek Oh , Jeong-Geun Kim , Kap-Hoon Han , Chang-Won Lee , Jae Won Kim; J. Microbiol. 2014;52(7):590-596. Published online June 28, 2014; DOI: https://doi.org/10.1007/s12275-014-3651-x

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To know the function of the plcA gene, which encodes a putative phosphoinositide-specific phospholipase C, in a model filamentous fungus Aspergillus nidulans, it was disrupted thorough homologous recombination and examined. The germination rate of ΔplcA was reduced by approximately 65% and germination of ΔplcA at a lower temperature (25°C) was much slower than germination under normal conditions (37°C), suggesting the plcA is responsible for cold-sensitivity. The hyphal growth of ΔplcA was slightly reduced at 37°C and conspicuously reduced at 25°C. While germinating ΔplcA formed giant swollen spores, and generated short and thick hyphae. The results of the nuclear examination of ΔplcA showed nuclear division with missegregation, and the rate of nuclear division was lower than that of wild type at both 25°C and 37°C. The results of this study showed that plcA is localized to the nucleus through intracellular calcium signaling in A. nidulans. The abnormal nuclear division, resulting from plcA gene deletion, affects conidiation in asexual development. Taken together, these results suggested that plcA is required for normal vegetative growth, morphogenesis, conidiation, and nuclear division
in A. nidulans.

Citations

Citations to this article as recorded by

Regulators of the Asexual Life Cycle of Aspergillus nidulans
Ye-Eun Son, Jae-Hyuk Yu, Hee-Soo Park
Cells.2023; 12(11): 1544. CrossRef
The Kinetochore Protein Spc105, a Novel Interaction Partner of LaeA, Regulates Development and Secondary Metabolism in Aspergillus flavus
Qing-Qing Zhi, Lei He, Jie-Ying Li, Jing Li, Zhen-Long Wang, Guang-Yao He, Zhu-Mei He
Frontiers in Microbiology.2019;[Epub] CrossRef

Research Support, Non-U.S. Gov't

Safety Evaluation of Lactobacillus paracasei subsp. paracasei LC-01, Probiotic Bacterium: Hao Zhang , Yu Wang , Jing Sun , Zirui Guo , Huiyuan Guo , Fazheng Ren; J. Microbiol. 2013;51(5):633-638. Published online October 31, 2013; DOI: https://doi.org/10.1007/s12275-013-3336-x

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Abstract

The safety of Lactobacillus paracasei subsp. paracasei LC-01 was evaluated for its use as a potential probiotic. In our in vitro study, the antibiotic resistance and the ability to produce biogenic amine were determined. The results showed that the strain was sensitive to all tested antibiotics and did not produce biogenic amine except for tyramine. The oral toxicity of this strain was evaluated in Balb/C mice. One hundred mice were divided into 10 groups. Four groups were administered 0, 108, 109, or 1010 CFU/mouse per day dissolved in saline solution respectively, for 28 days. Three groups were injected intraperitoneally with 109 CFU/mouse dissolved in saline solution, and were killed 2, 5, and 10 days after injection. The last 3 groups were injected with the vehicle as controls respectively. The results showed that oral administration of the strain had no adverse effects on mouse body weight and that there was no treatment-associated bacterial translocation. Intraperitoneal administration caused a significant translocation to liver, spleen and kidney. However, this translocation did not cause illness or death throughout the experiment. The results suggest that L. paracasei subsp. paracasei LC-01 is likely to be safe for human consumption.

Citations

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Genome sequence and evaluation of safety and probiotic potential of Lacticaseibacillus paracasei LC86 and Lacticaseibacillus casei LC89
Ting Chen, Yunjiao Zhao, Yixuan Fan, Yao Dong, Zhonghui Gai
Frontiers in Microbiology.2025;[Epub] CrossRef
Lactobacillus paracasei R3 Alleviates Tumor Progression in Mice with Colorectal Cancer
Tao Chen, Baoxia Li, Kangdi Zheng, Yan Liu, Zhao Zhang, Huimei Hu, Guoqiang Qian, Jianwei Jiang
Current Microbiology.2024;[Epub] CrossRef
Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99
Ying Wu, Zhiyi Wu, Yinyin Gao, Yixuan Fan, Yao Dong, Yinan Zhang, Zhonghui Gai, Shaobin Gu
Scientific Reports.2024;[Epub] CrossRef
Assessment of the Safety and Probiotic Properties of Enterococcus faecium B13 Isolated from Fermented Chili
Jingmin Xiao, Cai Chen, Zhuxian Fu, Shumin Wang, Fan Luo
Microorganisms.2024; 12(5): 994. CrossRef
Lactic acid bacteria with a strong antioxidant function isolated from “Jiangshui,” pickles, and feces
Yue Hu, Yan Zhao, Xu Jia, Dan Liu, Xinhe Huang, Cheng Wang, Yanhua Zhu, Changwu Yue, Shanshan Deng, Yuhong Lyu
Frontiers in Microbiology.2023;[Epub] CrossRef
Assessment of the Safety of Lactobacillus casei IMV B-7280 Probiotic Strain on a Mouse Model
Lazarenko L.M., Babenko L.P., Gichka S.G., Sakhno L.O., Demchenko O.M., Bubnov R.V., Sichel L.M., Spivak M.Ya.
Probiotics and Antimicrobial Proteins.2021; 13(6): 1644. CrossRef
Health benefits and technological effects of Lacticaseibacillus casei-01: An overview of the scientific literature
Tatiana Colombo Pimentel, Larissa Ramalho Brandão, Matthaws Pereira de Oliveira, Whyara Karoline Almeida da Costa, Marciane Magnani
Trends in Food Science & Technology.2021; 114: 722. CrossRef
Lactobacillus casei LC01 Regulates Intestinal Epithelial Permeability through miR-144 Targeting of OCLN and ZO1
Qiuke Hou, Yongquan Huang, Yan Wang, Liu Liao, Zhaoyang Zhu, Wenjie Zhang, Yongshang Liu, Peiwu Li, Xinlin Chen, Fengbin Liu
Journal of Microbiology and Biotechnology.2020; 30(10): 1480. CrossRef
Combinations of cereal β-glucans and probiotics can enhance the anti-inflammatory activity on host cells by a synergistic effect
Mattia Pia Arena, Pasquale Russo, Vittorio Capozzi, Ana Rascón, Giovanna E. Felis, Giuseppe Spano, Daniela Fiocco
Journal of Functional Foods.2016; 23: 12. CrossRef

Journal Article

Possible Translocation of Periodontal Pathogens into the Lymph Nodes Draining the Oral Cavity: G. Amodini Rajakaruna , Makoto Umeda , Keisuke Uchida , Asuka Furukawa , Bae Yuan , Yoshimi Suzuki , Ebe Noriko , Yuichi Izumi , Yoshinobu Eishi; J. Microbiol. 2012;50(5):827-836. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2030-8

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Abstract: Numerous publications have reported the presence of periodontopathogenic bacteria in peripheral and central vascular lesions. However, it is unclear how this bacterial translocation occurs. The objective of this study was to investigate whether periodontopathic bacteria are translocated to lymph nodes proximal to the oral cavity. Obtaining lymph node samples is not ethically feasible unless they are excised as part of the surgical management of patients with cancer. This study analyzed formalin-fixed and paraffin-embedded lymph nodes, histologically negative for cancer cell invasion, that were excised from 66 patients with histories of head and neck cancer. Real-time PCR was performed to amplify the 16S ribosomal DNA fragments from Porphyromonas gingivalis, Treponema denticola, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, and Prevotella intermedia. The relationship between bacterial detection and cancer severity, gender, and the use of anti-cancer therapy was examined by Fisher’s exact test. P. gingivalis, T. forsythia, and P. intermedia were present in 17%, 8%, and 8% of the samples of submandibular and submental lymph nodes, respectively. There were no significant relationships between bacterial detection and the cancer disease status, patient gender or use of anticancer therapy. According to these data, it appears that the translocation of periodontopathic bacteria may occur via lymphatic drainage, irrespective of the cancer disease status, gender or anticancer therapy.

Research Support, Non-U.S. Gov't

Translocation of Green Fluorescent Protein to Cyanobacterial Periplasm Using Ice Nucleation Protein: Wipa Chungjatupornchai , Sirirat Fa-aroonsawat; J. Microbiol. 2009;47(2):187-192. Published online May 2, 2009; DOI: https://doi.org/10.1007/s12275-008-0188-x

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Abstract: The translocation of proteins to cyanobacterial cell envelope is made complex by the presence of a highly differentiated membrane system. To investigate the protein translocation in cyanobacterium Synechococcus PCC 7942 using the truncated ice nucleation protein (InpNC) from Pseudomonas syringae KCTC 1832, the green fluorescent protein (GFP) was fused in frame to the carboxyl-terminus of InpNC. The fluorescence of GFP was found almost entirely as a halo in the outer regions of cells which appeared to correspond to the periplasm as demonstrated by confocal laser scanning microscopy, however, GFP was not displayed on the outermost cell surface. Western blotting analysis revealed that InpNC-GFP fusion protein was partially degraded. The N-terminal domain of InpNC may be susceptible to protease attack; the remaining C-terminal domain conjugated with GFP lost the ability to direct translocation across outer membrane and to act as a surface display motif. The fluorescence intensity of cells with periplasmic GFP was approximately 6-fold lower than that of cells with cytoplasmic GFP. The successful translocation of the active GFP to the periplasm may provide a potential means to study the property of cyanobacterial periplasmic substances in response to environmental changes in a non-invasive manner.

Journal Article

Growth and Physiological Properties of Wild Type and Mutants of Halomonas subglaciescola DH-1 in Saline Environment: Hye Jeong Ryu , Yoo Jung Jeong , Doo Hyun Park; J. Microbiol. 2004;42(3):174-180.; DOI: https://doi.org/2093 [pii]

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Abstract: A halophilic bacterium was isolated from fermented seafood. The 16S rDNA sequence identity between the isolate and Halomonas subglaciescola AJ306801 was above 95%. The isolate that did not grow in the condition without NaCl or in the condition with other sodium (Na^+) or chloride ions (Cl^-) instead of NaCl was named H. subglaciescola DH-1. Two mutants capable of growing without NaCl were obtained by random mutagenesis, of which their total soluble protein profiles were compared with those of the wild type by two-dimensional electrophoresis. The external compatible solutes (betaine and choline) and cell extract of the wild type did not function as osmoprotectants, and these parameters within the mutants did not enhance their growth in the saline environment. In the proton translocation test, rapid acidification of the reactant was not detected for the wild type, but it was detected for the mutant in the condition without NaCl. From these results, we derived the hypothesis that NaCl may be absolutely required for the energy metabolism of H. subglaciescola DH-1 but not for its osmoregulation, and the mutants may have another modified proton translocation system that is independent of NaCl, except for those mutants with an NaCl-dependent system.

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