Journal of Microbiology

Journal Article

NOTE] Identification of PhoB Binding Sites of the yibD and ytfK Promoter Regions in Escherichia coli: Yusuke Yoshida , Shinichiro Sugiyama , Tomoya Oyamada , Katsushi Yokoyama , Soo-Ki Kim , Kozo Makino; J. Microbiol. 2011;49(2):285-289. Published online May 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0360-6

Abstract: By using a lacZ operon fusion genomic library of the Escherichia coli O157:H7 Sakai, we identified phosphatestarvation-inducible (psi) promoters located upstream of the yibD and ytfK genes. They have been previously proposed to belong to the phosphate regulon (pho regulon) by Beak and Lee (2006), based on the DNA array and in vivo transcriptional experiments. However, the direct interaction of these promoters with the activator protein of the pho regulon, PhoB, has not been determined. We determined the binding regions of PhoB in these promoter regions by DNase I footprinting. Both regions contained two pho boxes similar to the consensus sequence for PhoB binding.

Research Support, Non-U.S. Gov't

Expression of Escherichia coli DcuS-R Two-Component Regulatory System is Regulated by the Secondary Internal Promoter Which is Activated by CRP-cAMP: Tomoya Oyamada , Katsushi Yokoyama , Michiko Morinaga , Masashi Suzuki , Kozo Makino; J. Microbiol. 2007;45(3):234-240.; DOI: https://doi.org/2537 [pii]

Abstract: The DcuS-R two-component system of Escherichia coli senses C4-dicarboxylates of the medium and regulates expression of the genes related to utilization of them. It is known that phospho-DcuR induces expression of genes such as the dcuB-fumB operon, the frdABCD operon, and the dctA gene. We analyzed promoters of the dcuS-R operon to elucidate the transcriptional regulation system. We found a novel internal promoter within the dcuS gene that is regulated by the transcriptional regulator, CRP-cAMP, in both aerobic and anaerobic conditions.

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