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- Antimicrobial Efficacy of Allium cepa and Zingiber officinale Against the Milk‑Borne Pathogen Listeria monocytogenes
- Abirami Arasu , Nagaram Prabha , Durga Devi , Praveen Kumar Issac , Khaloud Mohammed Alarjani , Dunia A. Al Farraj , Reem A. Aljeidi , Dina S. Hussein , Magesh Mohan , Jehad Zuhair Tayyeb , Ajay Guru , Jesu Arockiaraj
- J. Microbiol. 2023;61(11):993-1011. Published online December 4, 2023
- DOI: https://doi.org/10.1007/s12275-023-00086-w
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Abstract
- Listeria monocytogenes is an important food-borne pathogen that causes listeriosis and has a high case fatality rate despite its low incidence. Medicinal plants and their secondary metabolites have been identified as potential antibacterial substances, serving as replacements for synthetic chemical compounds. The present studies emphasize two significant medicinal plants, Allium cepa and Zingiber officinale, and their efficacy against L. monocytogenes. Firstly, a bacterial isolate was obtained from milk and identified through morphology and biochemical reactions. The species of the isolate were further confirmed through 16S rRNA analysis. Furthermore, polar solvents such as methanol and ethanol were used for the extraction of secondary metabolites from A. cepa and Z. officinale. Crude phytochemical components were identified using phytochemical tests, FTIR, and GC–MS. Moreover, the antibacterial activity of the crude extract and its various concentrations were tested against L. monocytogenes. Among all, A. cepa in methanolic extracts showed significant inhibitory activity. Since, the A. cepa for methanolic crude extract was used to perform autography to assess its bactericidal activity. Subsequently, molecular docking was performed to determine the specific compound inhibition. The docking results revealed that four compounds displayed strong binding affinity with the virulence factor Listeriolysin-O of L. monocytogenes. Based on the above results, it can be concluded that the medicinal plant A. cepa has potential antibacterial effects against L. monocytogenes, particularly targeting its virulence.
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Citations
Citations to this article as recorded by
- Cultural Perspectives on the Sustainable Use and Added Value of Plant-Based Food Dyes—A Case Study from Bulgaria
Mihail Chervenkov, Teodora Ivanova, Yulia Bosseva, Dessislava Dimitrova
Sustainability.2024; 16(20): 9049. CrossRef
- Cultural Perspectives on the Sustainable Use and Added Value of Plant-Based Food Dyes—A Case Study from Bulgaria
- Biofilm characterization of Fusarium solani keratitis isolate: increased resistance to antifungals and UV light
- Itzel Margarita Córdova-Alcántara , Diana Laura Venegas-Cortés , María Ángeles Martínez-Rivera , Néstor Octavio Pérez , Aida Verónica Rodriguez-Tovar
- J. Microbiol. 2019;57(6):485-497. Published online May 27, 2019
- DOI: https://doi.org/10.1007/s12275-019-8637-2
- 42 View
- 0 Download
- 39 Web of Science
- 40 Crossref
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Abstract
- Fusarium solani has drawn phytopathogenic, biotechnological, and medical interest. In humans, it is associated with localized infections, such as onychomycosis and keratomycosis, as well as invasive infections in immunocompromised patients. One pathogenicity factor of filamentous fungi is biofilm formation. There is still only scarce information about the in vitro mechanism of the formation and composition of F. solani biofilm. In this work, we describe the biofilm formed by a clinical keratomycosis isolate in terms of its development, composition and susceptibility to different antifungals and ultraviolet light (UV) at different biofilm formation stages. We found five biofilm formation stages using scanning electron microscopy: adherence, germination, hyphal development, maturation, and cell detachment. Using epifluorescence microscopy with specific fluorochromes, it was elucidated that the extracellular matrix consists of carbohydrates, proteins, and extracellular DNA. Specific inhibitors for these molecules showed significant biofilm reductions. The antifungal susceptibility against natamycin, voriconazole, caspofungin, and amphotericin B was evaluated by metabolic activity and crystal violet assay, with the F. solani biofilm preformation to 24 h increased in resistance to natamycin, voriconazole, and caspofungin, while the biofilm preformation to 48 h increased in resistance to amphotericin B. The preformed biofilm at 24 h protected and reduced UV light mortality. F. solani isolate could produce a highly structured extra biofilm; its cellular matrix consists of carbohydrate polymers, proteins, and eDNA. Biofilm confers antifungal resistance and decreases its susceptibility to UV light. The fungal biofilm functions as a survival strategy against antifungals and environmental factors.
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Citations
Citations to this article as recorded by- Trans-kingdom fungal pathogens infecting both plants and humans, and the problem of azole fungicide resistance
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Journal of Applied Microbiology.2023;[Epub] CrossRef - Evaluation of the performance of TiO2 thin films doped with silver nanoparticles as a protective coating for metal prostheses
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Surface and Coatings Technology.2023; 458: 129349. CrossRef - Effect of Voriconazole on Biofilm of Filamentous Species Isolated from Keratitis
Mahsa Fattahi, Reza Ghasemi, Olga Pinegina, Mohammad Mahdi Bahrami, Mahdi Hosseini, Ensieh Lotfali
Archives of Clinical Infectious Diseases.2022;[Epub] CrossRef - Advances in application of ultraviolet irradiation for biofilm control in water and wastewater infrastructure
Xueru Luo, Baoping Zhang, Yinghua Lu, Yang Mei, Liang Shen
Journal of Hazardous Materials.2022; 421: 126682. CrossRef - Biofilm Formation by Chromoblastomycosis Fungi Fonsecaea pedrosoi and Phialophora verrucosa: Involvement with Antifungal Resistance
Ingrid S. Sousa, Thaís P. Mello, Elaine P. Pereira, Marcela Q. Granato, Celuta S. Alviano, André L. S. Santos, Lucimar F. Kneipp
Journal of Fungi.2022; 8(9): 963. CrossRef - In vitro ability of Fusarium keratoplasticum to form biofilms in venous catheter
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Survey of Ophthalmology.2022; 67(3): 758. CrossRef - Biofilm Formation and Structure in the Filamentous Fungus Fusarium graminearum, a Plant Pathogen
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