Journal of Microbiology

Article

Extracellular products-mediated interspecific interaction between Pseudomonas aeruginosa and Escherichia coli: Yang Yuan , Jing Li , Jiafu Lin , Wenjuan Pan , Yiwen Chu , Balakrishnan Prithiviraj , Yidong Guo , Xinrong Wang , Kelei Zhao; J. Microbiol. 2021;59(1):29-40. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0478-0

485 View
2 Download
11 Web of Science
10 Crossref

The Gram-negative pathogen Pseudomonas aeruginosa adopts several elaborate strategies to colonize a wide range of natural or clinical niches and to overcome the neighboring bacterial competitors in polymicrobial communities. However, the relationship and interaction mechanism of P. aeruginosa with other bacterial pathogens remains largely unexplored. Here we explore the interaction dynamics of P. aeruginosa and Escherichia coli, which frequently coinfect the lungs of immunocompromised hosts, by using a series of on-plate proximity assays and RNA-sequencing. We show that the extracellular products of P. aeruginosa can inhibit the growth of neighboring E. coli and induce a large-scale of transcriptional reprogramming of E. coli, especially in terms of cellular respiration- related primary metabolisms and membrane components. In contrast, the presence of E. coli has no significant effect on the growth of P. aeruginosa in short-term culture, but causes a dysregulated expression of genes positively controlled by the quorum-sensing (QS) system of P. aeruginosa during subsequent pairwise culture. We further demonstrate that the divergent QS-regulation of P. aeruginosa may be related to the function of the transcriptional regulator PqsR, which can be enhanced by E. coli culture supernatant to increase the pyocyanin production by P. aeruginosa in the absence of the central las-QS system. Moreover, the extracellular products of E. coli promote the proliferation and lethality of P. aeruginosa in infecting the Caenorhabditis elegans model. The current study provides a general characterization of the extracellular products-mediated interactions between P. aeruginosa and E. coli, and may facilitate the understanding of polymicrobial infections.

Citations

Citations to this article as recorded by

YXL-13 attenuates virulence in Pseudomonas aeruginosa mutants and clinical isolates by inhibiting quorum sensing regulators LasR and RhlR
Lei Wang, Rui Luo, Xinlin Yan, Mengliang Han, Mingjie Zhang, Junhai Xiao, Kelei Zhao, Feng Lin
Microbial Pathogenesis.2026; 212: 108298. CrossRef
Metabolic interactions between bacterial co-isolates from catheter-associated urinary tract infections
Dmytro Sokol, Olena Rzhepishevska, Iryna Marynova, Tor Monsen, Henrik Antti, Madeleine Ramstedt
Scientific Reports.2026;[Epub] CrossRef
SERS-based approaches in the investigation of bacterial metabolism, antibiotic resistance, and species identification
Zhun Nie, Zhijun Huang, Zhongying Wu, Yanlong Xing, Fabiao Yu, Rui Wang
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.2025; 336: 126051. CrossRef
Impact of Clostridium butyricum HADIG-CB003 dietary supplementation on the gut microbiota of Kunming mice
Yilin Pang, Xianxian Xu, Daizhi Li, Yue Ge, Jiamin Liang, Xiaojun Xiong, Shaohuai Wen, Xiaokai Li, Yueqiu Wang, Xiongying Li, Guoqiang Tan, Yuanshan Lin
Applied Microbiology and Biotechnology.2025;[Epub] CrossRef
A fluorescence-based protocol to monitor bacterial antagonism
Justin M. Luu, Cristian V. Crisan, Morgan L. Pettis, Anayancy Ramos Facio, Timothy D. Read, Joanna B. Goldberg, Justin R. Kaspar
Microbiology Spectrum.2025;[Epub] CrossRef
The bacterial spectrum of spinal infections based on blood culture, tissue culture, and molecular methods: a systematic review and meta-analysis
Rui Zhang, Mengdie Wang, Xinyu Liu, Feifei Yang, Xinyuan Xu, Liye Zi, Zhi Liang, Xiaorong Liu, Hongbing Gao, Xuesong Chen, Guozhong Zhou
Scientific Reports.2025;[Epub] CrossRef
Rapid culture-free species identification and phenotypic antibiotic susceptibility test for polymicrobial infection by single-cell Raman spectroscopy
Miao Yu, Liliang Wang, Yiwen Xu, Jiyong Yang, Changsong Zhang, Ting Wang, Siyu Meng, Jingkai Wang, Qiang Zhao, Zhiqiang Zhang, Huijie Hu, Huan Zeng, Xianchao Zhou, Aiguo Shen, Junjie Tu, Chongyu Zhang, Jian He, Yuguo Tang, Yizhi Song
Chemical Engineering Journal.2025; 526: 171537. CrossRef
Pigments from pathogenic bacteria: a comprehensive update on recent advances
Kusumita Acharya, Swarna Shaw, Sudipta Paul Bhattacharya, Shatarupa Biswas, Suman Bhandary, Arijit Bhattacharya
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef
Selective detection of two bacterial species in a single collision system targeting metabolic products
Jun Lin, Qingwen Wang, Huike Tian, Qing Xin, Dong Zhang
Microchemical Journal.2024; 206: 111572. CrossRef
Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
Hongyan Cai, Jiaying Yu, Ying Qiao, Ying Ma, Jiang Zheng, Mao Lin, Qingpi Yan, Lixing Huang
Microorganisms.2022; 10(12): 2307. CrossRef

First
Prev
Page of 1
Next
Last

Search