Journal of Microbiology

Journal Article

Detection of colistin-resistant populations prior to antibiotic exposure in KPC-2-producing Klebsiella pneumoniae clinical isolates: Jungyu Seo , Yu Mi Wi , Jong Min Kim , Yae-Jean Kim , Kwan Soo Ko; J. Microbiol. 2021;59(6):590-597. Published online March 29, 2021; DOI: https://doi.org/10.1007/s12275-021-0610-1

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Although colistin is frequently regarded as the antibiotic of last resort in treating carbapenem-resistant Klebsiella pneumoniae, colistin heteroresistance may in part be associated with antibiotic treatment failure. However, we do not know how widespread the colistin heteroresistance is in carbapenem- resistant K. pneumoniae isolates. In this study, we performed colistin disc diffusion assays, E-tests, and population analysis profiling for KPC-2-producing K. pneumoniae isolates to identify colistin heteroresistance. Although no colistin- resistant colonies were detected by the disc diffusion test and E-test, a colistin-resistant subpopulation was identified in population analysis profiling in all colistin-susceptible, KPC-2-producing K. pneumoniae isolates. Colistin-resistant subpopulations were also identified even when isolates had no colistin exposure. The ratio of colistin-resistant subpopulations to the total population increased as the exposure concentration of colistin increased. In in vitro time-kill assays, regrowth was observed in all isolates after 2 h upon exposure to colistin. We identified common amino acid alterations in PhoQ, PhoP, and PmrB in colistin-resistant subpopulations from some isolates, but no substitutions were found in most resistant subpopulations from other isolates. In all colistin-resistant subpopulations, overexpression of PhoQ and PbgP was observed. In this study, we demonstrated that colistin heteroresistance may be common in KPC-2-producing K. pneumoniae isolates, which could not be detected in the disc diffusion method and E-test. Colistin heteroresistance may cause colistin treatment failure in part and may evolve into resistance. Thus, development of more reliable diagnostic methods is required to detect colistin heteroresistance.

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High prevalence of polymyxin-heteroresistant carbapenem-resistant Klebsiella pneumoniae and its within-host evolution to resistance among critically ill scenarios
Xiaoli Wang, Tianjiao Meng, Yunqi Dai, Hong-Yu Ou, Meng Wang, Bin Tang, Jingyong Sun, Decui Cheng, Tingting Pan, Ruoming Tan, Hongping Qu
Infection.2024;[Epub] CrossRef
Development of colistin resistance via heteroresistance modeling in Klebsiella pneumoniae: A diagnostic study
Jungyu Seo, Kwan Soo Ko
Precision and Future Medicine.2024; 8(1): 10. CrossRef
Conversion to colistin susceptibility by tigecycline exposure in colistin-resistant Klebsiella pneumoniae and its implications to combination therapy
Suyeon Park, Jihyun Choi, Dongwoo Shin, Ki Tae Kwon, Si-Ho Kim, Yu Mi Wi, Kwan Soo Ko
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Insight into Antibiotic Synergy Combinations for Eliminating Colistin Heteroresistant Klebsiella pneumoniae
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Yifan Wang, Xinqian Ma, Lili Zhao, Yukun He, Wenyi Yu, Shining Fu, Wentao Ni, Zhancheng Gao
Frontiers in Microbiology.2022;[Epub] CrossRef
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Tropical Medicine and Infectious Disease.2022; 7(12): 414. CrossRef
Antibiotic Heteroresistance in Klebsiella pneumoniae
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International Journal of Molecular Sciences.2021; 23(1): 449. CrossRef
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Research Support, Non-U.S. Gov'ts

Trichoderma reesei Sch9 and Yak1 regulate vegetative growth, conidiation, and stress response and induced cellulase production: Xinxing Lv† , Weixin Zhang† , Guanjun Chen , Weifeng Liu; J. Microbiol. 2015;53(4):236-242. Published online January 31, 2015; DOI: https://doi.org/10.1007/s12275-015-4639-x

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Abstract

Protein kinases are key players in controlling many basic cellular processes in almost all the organisms via mediating signal transduction processes. In the present study, we characterized the cellulolytic Trichoderma reesei orthologs of Saccharomyces cerevisiae Sch9 and Yak1 by sequence alignment and functional analysis. The T. reesei Trsch9Δ and Tryak1Δ mutant strains displayed a decreased growth rate on different carbon sources and produced less conidia. The absence of these two kinases also resulted in different but abnormal polarized apical growth as well as sensitivity to various stresses. In addition, disruption of the genes Trsch9 or Tryak1 resulted in perturbation of cell wall integrity. Interestingly, while the induced production of cellulases was slightly compromised in the Trsch9Δ strain, the extracellular production of cellulases was significantly improved in the absence of Yak1. The results indicate that TrSch9 and TrYak1 play an important role in filamentous growth, stress response and induced production of cellulases in T. reesei.

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International Journal of Biological Macromolecules.2025; 307: 141944. CrossRef
Transcriptomics and co-expression network analysis revealing candidate genes for the laccase activity of Trametes gibbosa
Jie Chen, Yi Ye, Yujie Chi, Xin Hao, Qingquan Zhao
BMC Microbiology.2023;[Epub] CrossRef
Kinase POGSK-3β modulates fungal plant polysaccharide-degrading enzyme production and development
Ting Zhang, Han-Zhi Li, Wen-Tong Li, Di Tian, Yuan-Ni Ning, Xue Liang, Jing Tan, Yan-Hao Zhao, Xue-Mei Luo, Jia-Xun Feng, Shuai Zhao
Applied Microbiology and Biotechnology.2023; 107(11): 3605. CrossRef
Assessing the intracellular primary metabolic profile of Trichoderma reesei and Aspergillus niger grown on different carbon sources
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Frontiers in Fungal Biology.2022;[Epub] CrossRef
AGC/AKT Protein Kinase SCH9 Is Critical to Pathogenic Development and Overwintering Survival in Magnaporthe oryzae
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Journal of Fungi.2022; 8(8): 810. CrossRef
High-dose rapamycin exerts a temporary impact on T. reesei RUT-C30 through gene trFKBP12
Ai-Ping Pang, Haiyan Wang, Funing Zhang, Xin Hu, Fu-Gen Wu, Zhihua Zhou, Wei Wang, Zuhong Lu, Fengming Lin
Biotechnology for Biofuels.2021;[Epub] CrossRef
Candida glabrata Yap6 Recruits Med2 To Alter Glycerophospholipid Composition and Develop Acid pH Stress Resistance
Pei Zhou, Xiaoke Yuan, Hui Liu, Yanli Qi, Xiulai Chen, Liming Liu, Isaac Cann
Applied and Environmental Microbiology.2020;[Epub] CrossRef
Biocontrol potential of Trichoderma asperellum mutants T39 and T45 and their growth promotion of poplar seedlings
Ruiting Guo, Zhiying Wang, Chang Zhou, Ying Huang, Haijuan Fan, Yucheng Wang, Zhihua Liu
Journal of Forestry Research.2020; 31(3): 1035. CrossRef
Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa
Maria Augusta C. Horta, Nils Thieme, Yuqian Gao, Kristin E. Burnum-Johnson, Carrie D. Nicora, Marina A. Gritsenko, Mary S. Lipton, Karthikeyan Mohanraj, Leandro José de Assis, Liangcai Lin, Chaoguang Tian, Gerhard H. Braus, Katherine A. Borkovich, Monika
Frontiers in Microbiology.2019;[Epub] CrossRef
The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles
Vera Novy, Fredrik Nielsen, Bernhard Seiboth, Bernd Nidetzky
Biotechnology for Biofuels.2019;[Epub] CrossRef
STK-12 acts as a transcriptional brake to control the expression of cellulase-encoding genes in Neurospora crassa
Liangcai Lin, Shanshan Wang, Xiaolin Li, Qun He, J. Philipp Benz, Chaoguang Tian, Katherine A. Borkovich
PLOS Genetics.2019; 15(11): e1008510. CrossRef
Involvement of BcYak1 in the Regulation of Vegetative Differentiation and Adaptation to Oxidative Stress of Botrytis cinerea
Qianqian Yang, Jianan Zhang, Jicheng Hu, Xue Wang, Binna Lv, Wenxing Liang
Frontiers in Microbiology.2018;[Epub] CrossRef
Role of Trichoderma reesei mitogen-activated protein kinases (MAPKs) in cellulase formation
Mingyu Wang, Meiling Zhang, Ling Li, Yanmei Dong, Yi Jiang, Kuimei Liu, Ruiqin Zhang, Baojie Jiang, Kangle Niu, Xu Fang
Biotechnology for Biofuels.2017;[Epub] CrossRef
A copper-responsive promoter replacement system to investigate gene functions in Trichoderma reesei: a case study in characterizing SAGA genes
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The Aspergillus fumigatus SchASCH9 kinase modulates SakAHOG1 MAP kinase activity and it is essential for virulence
Patrícia Alves de Castro, Thaila Fernanda dos Reis, Stephen K. Dolan, Adriana Oliveira Manfiolli, Neil Andrew Brown, Gary W. Jones, Sean Doyle, Diego M. Riaño‐Pachón, Fábio Márcio Squina, Camila Caldana, Ashutosh Singh, Maurizio Del Poeta, Daisuke Hagiwar
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Effects of Exopolysaccharide Production on Liquid Vegetative Growth, Stress Survival, and Stationary Phase Recovery in Myxococcus xanthus: Wei Hu , Jing Wang , Ian McHardy , Renate Lux , Zhe Yang , Yuezhong Li , Wenyuan Shi; J. Microbiol. 2012;50(2):241-248. Published online April 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1349-5

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Abstract: Exopolysaccharide (EPS) of Myxococcus xanthus is a wellregulated cell surface component. In addition to its known functions for social motility and fruiting body formation on solid surfaces, EPS has also been proposed to play a role in multi-cellular clumping in liquid medium, though this phenomenon has not been well studied. In this report, we confirmed that M. xanthus clumps formed in liquid were correlated with EPS levels and demonstrated that the EPS encased cell clumps exhibited biofilm-like structures. The clumps protected the cells at physiologically relevant EPS concentrations, while cells lacking EPS exhibited significant reduction in long-term viability and resistance to stressful conditions. However, excess EPS production was counterproductive to vegetative growth and viable cell recovery declined in extended late stationary phase as cells became trapped in the matrix of clumps. Therefore, optimal EPS production by M. xanthus is important for normal physiological functions in liquid.

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