Growing evidence suggests that the gut microbiome is an important
contributor to metabolic diseases. Alterations in microbial
communities are associated with changes in lipid metabolism,
glucose homeostasis, intestinal barrier functions,
and chronic inflammation, all of which can lead to metabolic
disorders. Therefore, the gut microbiome may represent a
novel therapeutic target for obesity, type 2 diabetes, and nonalcoholic
fatty liver disease. This review discusses how gut microbes
and their products affect metabolic diseases and outlines
potential treatment approaches via manipulation of the
gut microbiome. Increasing our understanding of the interactions
between the gut microbiome and host metabolism
may help restore the healthy symbiotic relationship between
them.
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Fungal development is regulated by a variety of transcription
factors in Aspergillus nidulans. Previous studies demonstrated
that the NF-κB type velvet transcription factors regulate certain
target genes that govern fungal differentiation and cellular
metabolism. In this study, we characterize one of the
VosA/VelB-inhibited developmental genes called vidA, which
is predicted to encode a 581-amino acid protein with a C2H2
zinc finger domain at the C-terminus. Levels of vidA mRNA
are high during the early and middle phases of asexual development
and decrease during the late phase of asexual development
and asexual spore (conidium) formation. Deletion
of either vosA or velB results in increased vidA mRNA accumulation
in conidia, suggesting that vidA transcript accumulation
in conidia is repressed by VosA and VelB. Phenotypic
analysis demonstrated that deletion of vidA causes decreased
colony growth, reduced production of asexual spores,
and abnormal formation of sexual fruiting bodies. In addition,
the vidA deletion mutant conidia contain more trehalose
and β-glucan than wild type. Overall, these results suggest
that VidA is a putative transcription factor that plays a
key role in governing proper fungal growth, asexual and sexual
development, and conidia formation in A. nidulans.
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Fungal development and secondary metabolism are closely
associated via the activities of the fungal NK-kB-type velvet
regulators that are highly conserved in filamentous fungi.
Here, we investigated the roles of the velvet genes in the aflatoxigenic
fungus Aspergillus flavus. Distinct from other Aspergillus
species, the A. flavus genome contains five velvet genes,
veA, velB, velC, velD, and vosA. The deletion of velD blocks
the production of aflatoxin B1, but does not affect the formation
of sclerotia. Expression analyses revealed that vosA and
velB mRNAs accumulated at high levels during the late phase
of asexual development and in conidia. The absence of vosA
or velB decreased the content of conidial trehalose and the
tolerance of conidia to the thermal and UV stresses. In addition,
double mutant analyses demonstrated that VosA and
VelB play an inter-dependent role in trehalose biosynthesis
and conidial stress tolerance. Together with the findings of
previous studies, the results of the present study suggest that
the velvet regulators play the conserved and vital role in sporogenesis,
conidial trehalose biogenesis, stress tolerance, and
aflatoxin biosynthesis in A. flavus.
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Filamentous fungi produce a variety of secondary metabolites of diverse beneficial and detrimental activities to humankind. The genes required for a given secondary metabolite are typically arranged in a gene cluster. There is considerable evidence that secondary metabolite gene regulation is, in part, by transcriptional control through hierarchical levels of transcriptional regulatory elements involved in secondary metabolite cluster regulation. Identification of elements regulating secondary metabolism could potentially provide a means of increasing production of beneficial metabolites, decreasing production of detrimental metabolites, aid in the identification of ‘silent’ natural products and also contribute to a broader understanding of molecular mechanisms by which secondary metabolites are produced. This review summarizes regulation of secondary metabolism associated with transcriptional regulatory elements from a broad view as well as the tremendous advances in discovery of cryptic or novel secondary metabolites by genomic mining.
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Cys
2
His
2
Zinc Finger Transcription Factor BcabaR1 Positively Regulates Abscisic Acid Production in Botrytis cinerea
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