Journal Articles
- Evolution of a major bovine mastitic genotype (rpoB sequence type 10-2) of Staphylococcus aureus in cows
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Dae-Sung Ko , Danil Kim , Eun-Kyung Kim , Jae-Hong Kim , Hyuk-Joon Kwon
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J. Microbiol. 2019;57(7):587-596. Published online June 27, 2019
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DOI: https://doi.org/10.1007/s12275-019-8699-1
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Abstract
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Staphylococcus aureus is the major pathogen leading to bovine
mastitis globally while livestock-associated methicillin
resistant S. aureus (LA-MRSA) has become a potential threat
to public health. MRSA from bovine mastitis is not common
but a methicillin susceptible S. aureus (MSSA) genotype, rpoB
sequence type (RST)10-2 (RST10-2), is prevalent in Korea.
To date, many genomic sequences from S. aureus have been
elucidated, but the complete genome sequences of RST10-2
MSSA from bovine mastitis has never been reported. In this
study, we determined the complete genome sequence of two
RST10-2 MSSA that differ from each other in staphylococcal
protein A and molecular prophage types [PMB64-1 (t2489/
mPPT0) and PMB81-4 (t127/mPPT1-2-3)] and conducted
a comparative genomics study. The genomic sequences of
PMB64-1 and PMB81-4 were more homologous to the representative
human RST10-2 strains (MSSA476, MW2 etc.)
compared to other RSTs. Most of them shared five common
pseudogenes, along with high amino acid identity of four
variable virulence genes that were identified in this study.
However, PMB64-1 and PMB81-4 acquired different strainspecific
pseudogenes and mobile genetic elements than the
human strains. The unique pseudogene profile and high identity
of the virulence genes were verified in RST10-2 field strains
from bovine mastitis. Thus, bovine mastitic RST10-2 MSSA
may have an evolutionary relationship with the human RST10-
2 community-associated (CA) MSSA and CA-MRSA strains
but may have adapted to cows.
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Citations
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- Rapid Antibacterial Activity Assessment of Chimeric Lysins
Jin-Mi Park, Jun-Hyun Kim, Gun Kim, Hun-Ju Sim, Sun-Min Ahn, Kang-Seuk Choi, Hyuk-Joon Kwon
International Journal of Molecular Sciences.2024; 25(4): 2430. CrossRef - Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli
Eun-Jin Ha, Seung-Min Hong, Seung-Ji Kim, Sun-Min Ahn, Ho-Won Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Antibiotics.2023; 12(12): 1714. CrossRef - Genetic characterization of Staphylococcus aureus isolated from Norway rats in Boston, Massachusetts
Gracen R. Gerbig, Helen Piontkivska, Tara C. Smith, Ruairi White, Jean Mukherjee, Hayley Benson, Marieke Rosenbaum, Jessica H. Leibler
Veterinary Medicine and Science.2023; 9(1): 272. CrossRef - Rapid Screening and Comparison of Chimeric Lysins for Antibacterial Activity against Staphylococcus aureus Strains
Jin-Mi Park, Dae-Sung Ko, Hee-Soo Kim, Nam-Hyung Kim, Eun-Kyoung Kim, Young-Hye Roh, Danil Kim, Jae-Hong Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Antibiotics.2023; 12(4): 667. CrossRef - Comparative genomics of bovine mastitis-origin Staphylococcus aureus strains classified into prevalent human genotypes
Dae-Sung Ko, Nam-Hyung Kim, Eun-Kyung Kim, Eun-Jin Ha, Young-Hye Ro, Danil Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Research in Veterinary Science.2021; 139: 67. CrossRef
- Wild birds and urban pigeons as reservoirs for diarrheagenic Escherichia coli with zoonotic potential
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Clarissa A. Borges , Marita V. Cardozo , Livia G. Beraldo , Elisabete S. Oliveira , Renato P. Maluta , Kaline B. Barboza , Karin Werther , Fernando A. Ávila
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J. Microbiol. 2017;55(5):344-348. Published online March 9, 2017
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DOI: https://doi.org/10.1007/s12275-017-6523-3
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Abstract
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In order to describe the role of wild birds and pigeons in the transmission of shiga toxigenic Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) to humans and other animals, samples were collected from cloacae and oropharynx of free-living wild birds and free-living pigeons. Two STEC (0.8%) and five EPEC strains (2.0%) were isolated from wild birds and four EPEC strains (2.0%) were recovered from pi-geons. Serogroups, sequence types (STs) and virulence genes, such as saa, iha, lpfAO113, ehxA, espA, nleB and nleE, detected in this study had already been implicated in human and ani-mal diseases. Multidrug resistance (MDR) was found in 25.0% of the pigeon strains and in 57.0% of the wild bird strains; the wild birds also yielded one isolate carrying extended-spec-trum β-lactamases (ESBLs) gene blaCTX-M-8. The high varia-bility shown by PFGE demonstrates that there are no preva-lent E. coli clones from these avian hosts. Wild birds and pi-geons could act as carriers of multidrug-resistant STEC and EPEC and therefore may constitute a considerable hazard to human and animal health by transmission of these strains to the environment.
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Animals.2024; 14(21): 3048. CrossRef - Pathogenic Bacteria in Free-Living Birds, and Its Public Health Significance
Aleksandra Kobuszewska, Beata Wysok
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Shuangyu Li, Xinshuai Liu, Haoyu Zhao, Yuhua Zhang, Zheng Lu, Juan Wang, Ruichao Li, Peng Xie, Yibin Hu, Caiyuan Zhou, Qian Mao, Leilei Sun, Shanshan Li, Wenhui Wang, Fang Wang, Xinyu Liu, Tiantian Liu, Wei Pan, Chengbao Wang, Linzhu Ren
Transboundary and Emerging Diseases.2024;[Epub] CrossRef - Monitoring the genetic variation of some Escherichia coli strains in wild birds and cattle
Ghada A. Ibrahim, Ahmed M. Salah-Eldein, Mayasar I. Al-zaban, Amal S.A. El-Oksh, Elsayyad M. Ahmed, Doaa S. Farid, Enas M. Saad
Onderstepoort Journal of Veterinary Research.2023;[Epub] CrossRef - Identification and characterization of pathogenic and multidrug-resistant bacteria in feral pigeons surrounding a veterinary hospital in Minas Gerais, Brazil
Jordana Almeida Santana, Carolina Pantuzza Ramos, Brendhal Almeida Silva, Graciela Kunrath Lima, Alexandra Tiso Comerlato, Amanda Cristina Araújo, Salene Angelini Colombo, Gustavo Canesso Bicalho, Rodrigo Otávio Silveira Silva
Ciência Rural.2023;[Epub] CrossRef - Characterisation of typical enteropathogenic Escherichia coli (tEPEC) lineages and novel bfpA variants detected in Australian fruit bats (Pteropus poliocephalus)
Fiona McDougall, David Gordon, Roy Robins-Browne, Vicki Bennett-Wood, Wayne S.J. Boardman, Petra L. Graham, Michelle Power
Science of The Total Environment.2023; 902: 166336. CrossRef - Chlamydia psittaci in Faecal Samples of Feral Pigeons (Columba livia forma urbana) in Urban Areas of Lublin city, Poland
Katarzyna Kowalczyk, Angelina Wójcik-Fatla
Current Microbiology.2022;[Epub] CrossRef - Detection of blaOXA-48 and mcr-1 Genes in Escherichia coli Isolates from Pigeon (Columba livia) in Algeria
Lotfi Loucif, Widad Chelaghma, Esma Bendjama, Zineb Cherak, Meriem Khellaf, Asma Khemri, Jean-Marc Rolain
Microorganisms.2022; 10(5): 975. CrossRef - Zoonotic potential of Newcastle disease virus: Old and novel perspectives related to public health
Aziz Ul‐Rahman, Hafiz Muhammad Ishaq, Muhammad Asif Raza, Muhammad Zubair Shabbir
Reviews in Medical Virology.2022;[Epub] CrossRef - Source Areas as a Key Factor Contributing to the Recovery Time of Controlled Feral Pigeon (Columba livia var. domestica) Colonies in Low-Density Urban Locations
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Animals.2022; 12(9): 1056. CrossRef - Antimicrobial Resistance Analysis of Escherichia coli Isolated from Pigeons in Qingdao, Shandong Province, China
Anqi Wang, Changmin Hu
Genes.2022; 13(9): 1510. CrossRef - Epidemiological investigation of recurrent outbreaks of haemolytic uraemic syndrome caused by Shiga toxin-producingEscherichia coliserotype O55:H7 in England, 2014–2018
C. Sawyer, B. Vishram, C. Jenkins, F. Jorgensen, L. Byrne, A. F. W. Mikhail, T. J. Dallman, K. Carroll, L. Ahyow, Q. Vahora, G. Godbole, S. Balasegaram
Epidemiology and Infection.2021;[Epub] CrossRef - Wildlife as Sentinels of Antimicrobial Resistance in Germany?
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Frontiers in Veterinary Science.2021;[Epub] CrossRef - Characterization of multidrug-resistant avian pathogenic Escherichia coli: an outbreak in canaries
Angela Hitomi Kimura, Vanessa Lumi Koga, Luís Eduardo de Souza Gazal, Benito Guimarães de Brito, Kelly Cristina Tagliari de Brito, Armando Navarro-Ocaña, Gerson Nakazato, Renata Katsuko Takayama Kobayashi
Brazilian Journal of Microbiology.2021; 52(2): 1005. CrossRef - Antimicrobial resistance in Escherichia coli isolates from frugivorous (Eidolon helvum) and insectivorous (Nycteris hispida) bats in Southeast Nigeria, with detection of CTX-M-15 producing isolates
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Comparative Immunology, Microbiology and Infectious Diseases.2021; 75: 101613. CrossRef - First isolation of atypical enteropathogenic Escherichia coli from geese (Anser anser domestica) and first description of atypical EPEC from turkeys and pigeons in Hungary
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BMC Veterinary Research.2021;[Epub] CrossRef - In vitro transduction of antimicrobial resistance genes into Escherichia coli isolates from backyard poultry in Mexico
Juan Martín Talavera-González, Martín Talavera-Rojas, Edgardo Soriano-Vargas, Jesús Vázquez-Navarrete, Celene Salgado-Miranda
Canadian Journal of Microbiology.2021; 67(5): 415. CrossRef - Unveiling the Gut Microbiota and Resistome of Wild Cotton Mice,Peromyscus gossypinus, from Heavy Metal- and Radionuclide-Contaminated Sites in the Southeastern United States
Jesse C. Thomas, Troy J. Kieran, John W. Finger, Natalia J. Bayona-Vásquez, Adelumola Oladeinde, James C. Beasley, John C. Seaman, J. Vaun McArthur, Olin E. Rhodes, Travis C. Glenn, Kim M. Handley
Microbiology Spectrum.2021;[Epub] CrossRef - Carriage and Subtypes of Foodborne Pathogens Identified in Wild Birds Residing near Agricultural Lands in California: a Repeated Cross-Sectional Study
N. Navarro-Gonzalez, S. Wright, P. Aminabadi, A. Gwinn, T. V. Suslow, M. T. Jay-Russell, Andrew J. McBain
Applied and Environmental Microbiology.2020;[Epub] CrossRef - Shiga toxin‐producingEscherichia coliin the animal reservoir and food in Brazil
Alice Gonzalez, Aloysio Cerqueira
Journal of Applied Microbiology.2020; 128(6): 1568. CrossRef - Phänotypische Antibiotikaresistenzen von Bakterienisolaten aus Zier-, Zoo- und falknerisch gehaltenen Greifvögeln
Leonie Steger, Monika Rinder, Rüdiger Korbel
Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere.2020; 48(04): 260. CrossRef - Multidrug-resistant Escherichia coli and Salmonella spp. isolated from pigeons
Shah Jungy Ibna Karim, Mahfuzul Islam, Tahmina Sikder, Rubaya Rubaya, Joyanta Halder, Jahangir Alam
Veterinary World.2020; 13(10): 2156. CrossRef - Recent Updates on Outbreaks of Shiga Toxin-Producing Escherichia coli and Its Potential Reservoirs
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Olivia M. Smith, William E. Snyder, Jeb P. Owen
Biological Reviews.2020; 95(3): 652. CrossRef - Domestic and game pigeons as reservoirs for Escherichia coli harbouring antimicrobial resistance genes
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Preventive Veterinary Medicine.2019; 162: 136. CrossRef - Feral pigeon (Columba livia var. domestica) management in low-density urban areas: prevention is better than cure
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Research Support, Non-U.S. Gov'ts
- Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples
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Dharmendra Kumar Soni , Durg Vijai Singh , Suresh Kumar Dubey
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J. Microbiol. 2015;53(9):653-660. Published online August 1, 2015
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DOI: https://doi.org/10.1007/s12275-015-5243-9
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Abstract
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Listeria monocytogenes, a life-threatening pathogen, poses
severe risk during pregnancy, may cause abortion, fetal death
or neonatal morbidity in terms of septicemia and meningitis.
The present study aimed at characterizing L. monocytogenes
isolated from pregnant women based on serotyping, antibiotic
susceptibility, virulence genes, in vivo pathogenicity test and
ERIC- and REP-PCR fingerprint analyses. The results revealed
that out of 3700 human clinical samples, a total of 30 (0.81%)
isolates [12 (0.80%) from placental bit (1500), 18 (0.81%) from
vaginal swab (2200)] were positive for L. monocytogenes. All
the isolates belonged to serogroup 4b, and were + ve for
virulence genes tested i.e. inlA, inlC, inlJ, plcA, prfA, actA,
hlyA, and iap. Based on the mice inoculation tests, 20 isolates
showed 100% and 4 isolates 60% relative virulence while
6 isolates were non-pathogenic. Moreover, 2 and 10 isolates
were resistant to ciprofloxacin and cefoxitin, respectively,
while the rest susceptible to other antibiotics used in this
study. ERIC- and REP-PCR collectively depicted that the isolates
from placental bit and vaginal swab had distinct PCR
fingerprints except a few isolates with identical patterns. This
study demonstrates prevalence of pathogenic strains mostly
resistant to cefoxitin and/or ciprofloxacin. The results indicate
the importance of isolating and characterizing the pathogen
from human clinical samples as the pre-requisite for accurate
epidemiological investigations.
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Citations
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Infection and Drug Resistance.2024; Volume 17: 229. CrossRef - Systematic review ofListeria monocytogenesfrom food and clinical samples in Chinese mainland from 2010 to 2019
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- Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis
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Xue Song , Jing Guo , Wen-xiu Ma , Zhi-yuan Ji , Li-fang Zou , Gong-you Chen , Hua-song Zou
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J. Microbiol. 2015;53(5):330-336. Published online May 3, 2015
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DOI: https://doi.org/10.1007/s12275-015-4589-3
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Abstract
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To identify novel virulence genes, a mutant library of Xanthomonas
citri subsp. citri 29-1 was produced using EZ-Tn5
transposon and the mutants were inoculated into susceptible
grapefruit. Forty mutants with altered virulence phenotypes
were identified. Nine of the mutants showed a complete loss
of citrus canker induction, and the other 31 mutants resulted
in attenuated canker symptoms. Southern blot analysis revealed
that each of the mutants carried a single copy of Tn5.
The flanking sequence was identified by plasmid rescue and
18 different ORFs were identified in the genome sequence.
Of these 18 ORFs, seven had not been previously associated
with the virulence of X. citri subsp. citri and were therefore
confirmed by complementation analysis. Real-time PCR analysis
showed that the seven genes were upregulated when
the bacteria were grown in citrus plants, suggesting that the
expression of these genes was essential for canker development.
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- Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation
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eLife.2024;[Epub] CrossRef - The Methyltransferase HemK Regulates the Virulence and Nutrient Utilization of the Phytopathogenic Bacterium Xanthomonas citri Subsp. citri
Yu Shi, Xiaobei Yang, Xiaoxin Ye, Jiaying Feng, Tianfang Cheng, Xiaofan Zhou, Ding Xiang Liu, Linghui Xu, Junxia Wang
International Journal of Molecular Sciences.2022; 23(7): 3931. CrossRef -
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