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Biocontrol activity of volatile organic compounds from Streptomyces alboflavus TD-1 against Aspergillus flavus growth and aflatoxin production
Mingguan Yang , Laifeng Lu , Jing Pang , Yiling Hu , Qingbin Guo , Zhenjing Li , Shufen Wu , Huanhuan Liu , Changlu Wang
J. Microbiol. 2019;57(5):396-404.   Published online May 6, 2019
DOI: https://doi.org/10.1007/s12275-019-8517-9
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  • 43 Web of Science
  • 42 Crossref
AbstractAbstract
Aspergillus flavus is a saprophytic fungus that contaminates crops with carcinogenic aflatoxin. In the present work, the antifungal effects of volatile organic compounds (VOCs) from Streptomyces alboflavus TD-1 against A. flavus were investigated. VOCs from 8-day-old wheat bran culture of S. alboflavus TD-1 displayed strong inhibitory effects against mycelial growth, sporulation, and conidial germination of A. flavus. Severely misshapen conidia and hyphae of A. flavus were observed by scanning electron microscopy after exposure to VOCs for 6 and 12 h, respectively. Rhodamine 123 staining of mitochondria indicated that mitochondria may be a legitimate antifungal target of the VOCs from S. alboflavus TD-1. Furthermore, the VOCs effectively inhibited aflatoxin B1 production by downregulating genes involved in aflatoxin biosynthesis. Dimethyl trisulfide and benzenamine may play important roles in the suppression of A. flavus growth and production of aflatoxin. The results indicate that VOCs from S. alboflavus TD-1 have tremendous potential to be developed as a useful bio-pesticide for controlling A. flavus.

Citations

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    Physiological and Molecular Plant Pathology.2024; 134: 102440.     CrossRef
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    Zhenhui Jin, Yi-Cheng Wang
    Food Research International.2024; 190: 114550.     CrossRef
  • Suppression of Thielaviopsis ethacetica wilt and root rot, the emerging pathogen on bell pepper (Capsicum annuum), and plant growth promotion properties by the safety indigenous Streptomyces SBcT04
    Loan Le-Thi, Kim-Diep Tran, Hoai-Hieu Vo, Tu Nguyen-Van, Tam Nguyen-Thi, Anh-Truc Nong-Thi, Ngoc-Duong Vu, Nha-Hoa Phan
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    Piotr Siupka, Frederik Teilfeldt Hansen, Aleksandra Schier, Simone Rocco, Trine Sørensen, Zofia Piotrowska-Seget
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    Zahaed Evangelista-Martínez, Erika Anahí Contreras-Leal, Luis Fernando Corona-Pedraza, Élida Gastélum-Martínez
    Egyptian Journal of Biological Pest Control.2020;[Epub]     CrossRef
  • Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors
    Xianfeng Ren, Qi Zhang, Wen Zhang, Jin Mao, Peiwu Li
    Toxins.2020; 12(1): 24.     CrossRef
  • The Aspergilli and Their Mycotoxins: Metabolic Interactions With Plants and the Soil Biota
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    Gareth Thomas, David Withall, Michael Birkett
    Microbial Biotechnology.2020; 13(5): 1366.     CrossRef
  • Inhibitory Effects of Eurotium cristatum on Growth and Aflatoxin B1 Biosynthesis in Aspergillus flavus
    Qiannan Zhao, Yue Qiu, Xin Wang, Yuanyuan Gu, Yuzhu Zhao, Yidi Wang, Tianli Yue, Yahong Yuan
    Frontiers in Microbiology.2020;[Epub]     CrossRef
  • Effect of chlorogenic acid on controlling kiwifruit postharvest decay caused by Diaporthe sp.
    Danfeng Zhang, Wanling Bi, Kai Kai, Yingwang Ye, Jia Liu
    LWT.2020; 132: 109805.     CrossRef
  • Genome Mining Revealed a High Biosynthetic Potential for Antifungal Streptomyces sp. S-2 Isolated from Black Soot
    Piotr Siupka, Artur Piński, Dagmara Babicka, Zofia Piotrowska-Seget
    International Journal of Molecular Sciences.2020; 21(7): 2558.     CrossRef
  • Functional Application of Sulfur-Containing Spice Compounds
    Jinxin Xie, Benjian Liao, Ri-Yuan Tang
    Journal of Agricultural and Food Chemistry.2020; 68(45): 12505.     CrossRef
  • Efficacy of volatile compounds from Streptomyces philanthi RL‐1‐178 as a biofumigant for controlling growth and aflatoxin production of the two aflatoxin‐producing fungi on stored soybean seeds
    S. Boukaew, P. Prasertsan
    Journal of Applied Microbiology.2020; 129(3): 652.     CrossRef
  • Microenvironmental Interplay Predominated by Beneficial Aspergillus Abates Fungal Pathogen Incidence in Paddy Environment
    Xiaoyan Fan, Haruna Matsumoto, Yue Wang, Yang Hu, Yufei Liu, Hongda Fang, Bartosz Nitkiewicz, Sharon Yu Ling Lau, Qiangwei Wang, Hua Fang, Mengcen Wang
    Environmental Science & Technology.2019; 53(22): 13042.     CrossRef
Root-associated bacteria influencing mycelial growth of Tricholoma matsutake (pine mushroom)
Seung-Yoon Oh , Young Woon Lim
J. Microbiol. 2018;56(6):399-407.   Published online June 1, 2018
DOI: https://doi.org/10.1007/s12275-018-7491-y
  • 51 View
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  • 27 Crossref
AbstractAbstract
Tricholoma matsutake is an ectomycorrhizal fungus usually associated with Pinus densiflora in South Korea. Fruiting bodies (mushrooms) of T. matsutake are economically important due to their attractive aroma; yet, T. matsutake is uncultivatable and its habitat is rapidly being eradicated due to global climate change. Root-associated bacteria can influence the growth of ectomycorrhizal fungi that co-exist in the host rhizosphere and distinctive bacterial communities are associated with T. matsutake. In this study, we investigated how these bacterial communities affect T. matsutake growth by isolating bacteria from the roots of P. densiflora colonized by ectomycorrhizae of T. matsutake and co-culturing rootassociated bacteria with T. matsutake isolates. Thirteen species of bacteria (27 isolates) were found in pine roots, all belonging to the orders Bacillales or Burkholderiales. Two species in the genus Paenibacillus promoted the growth of T. matsutake in glucose poor conditions, likely using soluble metabolites. In contrast, other bacteria suppressed the growth of T. matsutake using both soluble and volatile metabolites. Antifungal activity was more frequent in glucose poor conditions. In general, pine rhizospheres harbored many bacteria that had a negative impact on T. matsutake growth and the few Paenibacillus species that promoted T. matsutake growth. Paenibacillus species, therefore, may represent a promising resource toward successful cultivation of T. matsutake.

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    Alec Mlambo, Mcebisi Maphosa
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Fumigant Activity of Volatiles from Streptomyces alboflavus TD-1 against Fusarium moniliforme Sheldon
Zhifang Wang , Changlu Wang , Fengjuan Li , Zhenjing Li , Mianhua Chen , Yurong Wang , Xi Qiao , Hong Zhang
J. Microbiol. 2013;51(4):477-483.   Published online August 30, 2013
DOI: https://doi.org/10.1007/s12275-013-2586-y
  • 36 View
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  • 35 Scopus
AbstractAbstract
The fumigant activity of volatiles generated by Streptomyces alboflavus TD-1 against Fusarium moniliforme Sheldon was investigated. The results showed that the mycelial growth, sporulation, and spore germination of F. moniliforme were significantly suppressed, and that membrane permeability was disrupted in the presence of the volatiles. Gas chromatography-mass Spectrometry analysis revealed 31 kinds of volatile organic compound from the volatiles. Among them, two earthy-smelling substances, namely, 2-methylisoborneol (50.97%) and trans-1,10-dimethyl-trans-9-decalinol (3.10%) were found. The most abundant compound, 2-methylisoborneol, exhibited inhibitory activity against F. moniliforme by fumigation. All these results suggested that S. alboflavus TD-1 can be a promising starter for the inhibition of F. moniliforme through fumigant action.
Research Support, Non-U.S. Gov't
Distinctive Endophytic Fungal Assemblage in Stems of Wild Rice (Oryza granulata) in China with Special Reference to Two Species of Muscodor (Xylariaceae)
Zhi-lin Yuan , Zhen-zhu Su , Li-juan Mao , Yang-qing Peng , Guan-mei Yang , Fu-cheng Lin , Chu-long Zhang
J. Microbiol. 2011;49(1):15-23.   Published online March 3, 2011
DOI: https://doi.org/10.1007/s12275-011-0213-3
  • 36 View
  • 0 Download
  • 30 Scopus
AbstractAbstract
Ecological niches in the rhizosphere and phyllosphere of grasses capable of sustaining endophytes have been extensively studied. In contrast, little information regarding the identity and functions of endophytic fungi in stems is available. In this study, we investigated the taxonomic affinities, diversity, and host specificities of culturable endophytes in stems of wild rice (Oryza granulata) in China. Seventy-four isolates were recovered. Low recovery rate (11.7%) indicated that there were relatively few sites for fungal infection. Identification using morphology, morphospecies sorting, and molecular techniques resulted in classification into 50 taxa, 36 of which were recovered only once. Nucleotide sequence similarity analysis indicated that 30% of the total taxa recovered were highly divergent from known species and thus may represent lineages new to science. Most of the taxa were classified as members of the classes Sordariomycetes or Dothideomycetes (mainly in Pleosporales). The presence of Arthrinium and Magnaporthaceae species, most often associated with poaceous plants, suggested a degree of host specificity. A polyphasic approach was employed to identify two Muscodor taxa based on (i) ITS and RPB2 phylogenies, (ii) volatile compounds produced, and (iii) an in vitro bioassay of antifungal activity. This to our knowledge is only the second report regarding the isolation of Muscodor spp. in China. Therefore, we hypothesize that wild plants represent a huge reservoir of unknown fungi. The prevalence, novelty, and species-specificity of unique isolates necessitate a reevaluation of their contribution to ecosystem function and fungal biodiversity.

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