Journal of Microbiology

Production and Characterization of Monoclonal Antibodies to Human Immunodeficiency Virus Type 1 gp120 Envelope Glycoprotein: Choi, Eui Yul , Ryu, Ji Yoon , Lee, Yoon , Ha, Sung Gil , Chung, So Young , Park, Sang Yeol , Nham, Sang Uk , Lee, Young Ik , Park, Jin Seu; J. Microbiol. 1998;36(1):59-65.

Abstract: Monoclonal antibodies to human immunodeficiency virus type 1 envelope glycoprotein gp 120(HIV-1 gp120) were produced and characterized. For immunogen recombinant gp120 polypeptide expressed in bacteria was prepared and injected into mice. From two fusion experiments, twenty hybridomas secreting monoclonal antibodies against the recombinant gp120 were initially screened by immunodot blot analysis. Among the antibodies, 15 of them showed strong reactivities with the recombinant protein expressed in bacteria in Western blot and thus it was tested if these could react with the recombinant protein expressed in insect cells. All of the 15 antibodies immunostained the protein band with varing degrees of reactivities. Next, we tested whether the antibodies recognize authentic gp120 protein expressed in mammalian cells. COS-1 cells were tranfected with the cDNA encoding gp120 protein, and the transiently ecpressed protein were analyzed with the mAbs by Western blot analysis and immunofluorescence microscopy. Six of the monoclonal antibodies reacted with the protein band of authentic gp120 expressed in mammalian cells in the Western blot, and five stained the cell periphery of the transfected COS-1 cells in immunofluorescence. The mAbs described in this study should prove to be useful tools for the biochemical, immunological and structural analysis of HIV-1 gp120 envelope glycoprotein.

Cellular Response of Pseudomonas sp. DJ-12 to the Stresses of Several Aromatic Pollutants: Park, Sang Ho , Ko, Yeon Ja , Oh, Kye Heon , Kim, Chi Kyung; J. Microbiol. 1998;36(2):93-98.

Abstract: Pseudomonas sp. DJ-12 is capable of degrading biphenyl, 4-chlorobiphenyl (4CB), and 4-hydroxybenzoate (4HBA) at relatively low concentrations. The degradability and cellular responses of the organism to the aromatic pollutants at higher concentrations have not been studied. In this study, the survival of the cells, production of stress proteins, and induction of tolerance were examined by exposing cells to various concentrations of the aromatics. The survival rates of the organism were reversely proportional to the concentration of each aromatic during incubation for 6 hs. Stress protein specifically reacting with anti-DnaK monoclonal antibody was commonly produced when cells were treated with each aromatic. Those cells pretreated with each aromatic at lower concentrations exhibited a certain degree of tolerance to higher concentrations of the aromatics. Such cellular responses of the organism to water-soluble 4HBA were more clearly distinguished than those to insoluble 3CB and biphenyl. Therefor, induction of DnaK stress protein in the cells by exposure to the aromatiocs could be used to develop an indicator for pollution by the aromatics.

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