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- The Role of Wheat Germ Agglutinin in the Attachment of Pseudomonas sp. WS32 to Wheat Root
- Jian Zhang , Liyuan Meng , Yuanyuan Cao , Huiping Chang , Zhongyou Ma , Leni Sun , Ming Zhang , Xinyun Tang
- J. Microbiol. 2014;52(12):1020-1024. Published online November 29, 2014
- DOI: https://doi.org/10.1007/s12275-014-4089-x
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Abstract
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Wheat germ agglutinin (WGA), which is secreted on the
surface of wheat root, has been defined as a protein that reversibly
and non-enzymatically binds to specific carbohydrates.
However, little attention has been paid to the function
of WGA in the attachment of bacteria to their host plants.
The aim of this study was to investigate the role of WGA in
the attachment of Pseudomonas sp. WS32 to wheat roots.
Wheat roots were initially treated with double-distilled water,
WGA-H (WGA solution that was heated at 100°C for 15 min)
and WGA, independently. Subsequently, the roots were coincubated
with cell solutions (109 cells/ml). A dilution plate
method
using a solid nutrient medium was employed to determine the adsorption of WS32 to wheat roots. WGA was labeled with fluorescein isothiocyanate and detected using the fluorescent in situ hybridization (FISH) technique. The number of adsorptive WS32 cells on wheat roots was significantly increased when the wheat roots were pretreated with WGA, compared with the control treatment (p = 0.01). However, WGA-H failed to increase the amount of bacterial cells that attached to the wheat roots because of the loss of its physiological activity. The FISH assay also revealed that more cells adhered to WGA-treated wheat roots than to control or WGA-H-treated roots. The results indicated that WGA can mediate Pseudomonas strain WS32’s adherence to wheat seedling roots. The findings of this study provide a better understanding of the processes involved in plant-microbe interactions. -
Citations
Citations to this article as recorded by
- Isolation and purification of wheat germ agglutinin and analysis of its properties
Han Wang
IOP Conference Series: Earth and Environmental Science.2017; 100: 012021. CrossRef
- Isolation and purification of wheat germ agglutinin and analysis of its properties
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