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- Cloning, Expression, and Characterization of Xylose Reductase with Higher Activity from Candida tropicalis
- Feiwei Zhang , Dairong Qiao , Hui Xu , Chong Liao , Shilin Li , Yi Cao
- J. Microbiol. 2009;47(3):351-357. Published online June 26, 2009
- DOI: https://doi.org/10.1007/s12275-008-0225-9
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- 17 Crossref
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Abstract
- Xylose reductase (XR) is a key enzyme in xylose metabolism because it catalyzes the reduction of xylose to xylitol. In order to study the characteristics of XR from Candida tropicalis SCTCC 300249, its XR gene(xyl1) was cloned and expressed in Escherichia coli BL21 (DE3). The fusion protein was purified effectively by Ni2+-chelating chromatography, and the kinetics of the recombinant XR was investigated. The Km values of the C. tropicalis XR for NADPH and NADH were 45.5 uM and 161.9 uM, respectively, which demonstrated that this XR had dual coenzyme specificity. Moreover, this XR showed the highest catalytic efficiency (kcat=1.44x04 min-1) for xylose among the characterized aldose reductases. Batch fermentation was performed with Saccharomyces serivisiae W303-1A:pYES2XR, and resulted in 7.63 g/L cell mass, 93.67 g/L xylitol, and 2.34 g/Lh xylitol productivity. This XR coupled with its dual coenzyme specificity, high activity, and catalytic efficiency proved its utility in in vitro xylitol production.
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Citations
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- Efficient production of ginsenoside Rh2 from xylose by remodeling metabolism in Saccharomyces cerevisiae
Wanze Zhang, Jiale Zhang, Xiaomeng Zhao, Zhanwei Zhang, Shifan He, Xueke Bian, Haibin Wang, Chuanbo Zhang, Wenyu Lu
Chemical Engineering Journal.2024; 494: 153120. CrossRef - Recent insights, applications and prospects of xylose reductase: a futuristic enzyme for xylitol production
Yogita Lugani, Munish Puri, Balwinder Singh Sooch
European Food Research and Technology.2021; 247(4): 921. CrossRef - Characterization of d-xylose reductase, XyrB, from Aspergillus niger
Agata Terebieniec, Tania Chroumpi, Adiphol Dilokpimol, Maria Victoria Aguilar-Pontes, Miia R. Mäkelä, Ronald P. de Vries
Biotechnology Reports.2021; 30: e00610. CrossRef - Combining Xylose Reductase from Spathaspora arborariae with Xylitol Dehydrogenase from Spathaspora passalidarum to Promote Xylose Consumption and Fermentation into Xylitol by Saccharomyces cerevisiae
Adriane Mouro, Angela A. dos Santos, Denis D. Agnolo, Gabriela F. Gubert, Elba P. S. Bon, Carlos A. Rosa, César Fonseca, Boris U. Stambuk
Fermentation.2020; 6(3): 72. CrossRef - Kinetics and Predicted Structure of a Novel Xylose Reductase from Chaetomium thermophilum
Julian Quehenberger, Tom Reichenbach, Niklas Baumann, Lukas Rettenbacher, Christina Divne, Oliver Spadiut
International Journal of Molecular Sciences.2019; 20(1): 185. CrossRef - Biosynthetic strategies to produce xylitol: an economical venture
Yirong Xu, Ping Chi, Muhammad Bilal, Hairong Cheng
Applied Microbiology and Biotechnology.2019; 103(13): 5143. CrossRef - A halotolerant aldose reductase from Debaryomyces nepalensis: gene isolation, overexpression and biochemical characterization
Bhaskar Paidimuddala, Gopala Krishna Aradhyam, Sathyanarayana N. Gummadi
RSC Advances.2017; 7(33): 20384. CrossRef - Inhibition of Debaryomyces nepalensis xylose reductase by lignocellulose derived by-products
Bhaskar Paidimuddala, Ashish Rathod, Sathyanarayana N. Gummadi
Biochemical Engineering Journal.2017; 121: 73. CrossRef - Bioprospecting and evolving alternative xylose and arabinose pathway enzymes for use in Saccharomyces cerevisiae
Sun-Mi Lee, Taylor Jellison, Hal S. Alper
Applied Microbiology and Biotechnology.2016; 100(5): 2487. CrossRef - The yeast Scheffersomyces amazonensis is an efficient xylitol producer
Raquel M. Cadete, Monaliza A. Melo-Cheab, Adriana L. Viana, Evelyn S. Oliveira, César Fonseca, Carlos A. Rosa
World Journal of Microbiology and Biotechnology.2016;[Epub] CrossRef - Identification and characterization of d-arabinose reductase and d-arabinose transporters from Pichia stipitis
Seiya Watanabe, Yuki Utsumi, Shigeki Sawayama, Yasuo Watanabe
Bioscience, Biotechnology, and Biochemistry.2016; 80(11): 2151. CrossRef - Sequence analysis of KmXYL1 genes and verification of thermotolerant enzymatic activities of xylose reductase from four Kluyveromyces marxianus strains
Jae-Bum Park, Jin-Seong Kim, Seung-Won Jang, Deok-Ho Kweon, Eock Kee Hong, Won Cheol Shin, Suk-Jin Ha
Biotechnology and Bioprocess Engineering.2016; 21(5): 581. CrossRef - Cloning, expression, and characterization of a novel xylose reductase from Rhizopus oryzae
Min Zhang, Shao‐tong Jiang, Zhi Zheng, Xing‐jiang Li, Shui‐zhong Luo, Xue‐feng Wu
Journal of Basic Microbiology.2015; 55(7): 907. CrossRef - Metabolic engineering strategies for improving xylitol production from hemicellulosic sugars
Buli Su, Mianbin Wu, Jianping Lin, Lirong Yang
Biotechnology Letters.2013; 35(11): 1781. CrossRef - Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777
Biao Zhang, Ling Zhang, Dongmei Wang, Xiaolian Gao, Jiong Hong
Journal of Industrial Microbiology & Biotechnology.2011; 38(12): 2001. CrossRef - Purification and biochemical characterization of a moderately halotolerant NADPH dependent xylose reductase from Debaryomyces nepalensis NCYC 3413
Sawan Kumar, Sathyanarayana N. Gummadi
Bioresource Technology.2011; 102(20): 9710. CrossRef - Cosubstrate effect on xylose reductase and xylitol dehydrogenase activity levels, and its consequence on xylitol production by Candida tropicalis
Elena Tamburini, Ercolina Bianchini, Alessandro Bruni, Giuseppe Forlani
Enzyme and Microbial Technology.2010; 46(5): 352. CrossRef
- Efficient production of ginsenoside Rh2 from xylose by remodeling metabolism in Saccharomyces cerevisiae
- Electrochemical Reduction of Xylose to Xylitol by Whole Cells or Crude Enzyme of Candida peltata
- Sun Mi Park , Byung In Sang , Dae Won Park , Doo Hyun Park
- J. Microbiol. 2005;43(5):451-455.
- DOI: https://doi.org/2276 [pii]
- 38 View
- 0 Download
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Abstract
- In this study, whole cells and a crude enzyme of Candida peltata were applied to an electrochemical bioreactor, in order to induce an increment of the reduction of xylose to xylitol. Neutral red was utilized as an electron mediator in the whole cell reactor, and a graphite-Mn(IV) electrode was used as a catalyst in the enzyme reactor in order to induce the electrochemical reduction of NAD+ to NADH. The efficiency with which xylose was converted to xylitol in the electrochemical bioreactor was five times higher than that in the conventional bioreactor, when whole cells were employed as a biocatalyst. Meanwhile, the xylose to xylitol reduction efficiency in the enzyme reactor using the graphite-Mn (IV) electrode and NAD+ was twice as high as that observed in the conventional bioreactor which utilized NADH as a reducing power. In order to use the graphite-Mn(IV) electrode as a catalyst for the reduction of NAD+ to NADH, a bioelectrocatalyst was engineered, namely, oxidoreductase (e.g. xylose reductase). NAD+ can function in this biotransformation procedure without any electron mediator or a second oxidoreductase for NAD+/NADH recycling
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