Journal of Microbiology

Journal Articles

Description of Fervidibacillus gen. nov. with Two Species, Fervidibacillus albus sp. nov., and Fervidibacillus halotolerans sp. nov., Isolated from Tidal Flat Sediments and Emendation of Misclassificed Taxa in the Genus Caldibacillus: Sung&# , Mi&# , Hyun&# , Kae Kyoung Kwon; J. Microbiol. 2023;61(2):175-187. Published online February 17, 2023; DOI: https://doi.org/10.1007/s12275-023-00022-y

56 View
0 Download
3 Web of Science
3 Crossref

Two Gram-stain-positive, motile, endospore-forming, facultatively anaerobic strains, designated MEBiC13591T and MEBiC13594T, were isolated from tidal flat sediment of the Incheon City on the west coast of Korea. Growth of both novel strains was observed at pH 5–9 (optimum, pH 7–7.5), and in 0–8% NaCl (optimum, 2% for MEBiC13591T and 3% for MEBiC13594T). Strains MEBiC13591T and MEBiC13594T grew optimally at 50 °C, (37.5–56.1 °C) and 44 °C (20.7–50.7 °C), respectively. The main cellular fatty acids of strain MEBiC13591T were iso-C15: 0, anteiso-C15: 0, iso-C16: 0, iso-C17: 0 and anteiso-C17: 0, while those for strain MEBiC13594T were C14: 0, iso-C14: 0, iso-C15: 0, anteiso-C15: 0 and C16: 0. In both taxa, the major isoprenoid was MK-7. The genomic DNA G + C contents were 34.1 and 37.0 mol% for MEBiC13591T and MEBiC13594T, respectively. A 16S rRNA gene sequence analysis revealed that the novel strains showed high similarity with members of the genera Aeribacillus (95.0%) and Caldibacillus (93.5–94.5%); however, showed lower than 90% with Caldibacillus debilis TfT. Phylogenetic and Phylogenomic analysis revealed that two novel strains comprised distinct phyletic line with members formerly assigned to Caldibacillus. Based on genomic indices, such as AAI and ANI, members formerly affiliated with Caldibacillus and Bacillus as well as the novel strains should be classified into five independent genera. Based on the phenotypic, genomic and biochemical data, strains MEBiC13591T and MEBiC13594T represent two novel species in the novel genus, for which the names Fervidibacillus albus gen. nov., sp. nov. ( MEBiC13591T [= KCCM 43317T = KCTC 43181T = JCM 33662T = MCCC 1K04565T]), and Fervidibacillus halotolerans sp. nov. ( MEBiC13594T [= KCTC 43182T = JCM 34001T]) are proposed. Three additional genera Caldifermentibacillus, Palidibacillus, and Perspicuibacillus are also proposed by reclassification of the several species with valid names that formerly affiliated with the genera Caldibacillus.

Citations

Citations to this article as recorded by

An update on novel taxa and revised taxonomic status of bacteria isolated from domestic companion and agricultural animals described in 2023
Sara D. Lawhon, Claire R. Burbick, Trinity Krueger, Elena Ruiz-Reyes, Erik Munson, Romney M. Humphries
Journal of Clinical Microbiology.2024;[Epub] CrossRef
Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. Within the OM60/NOR5 Clade, Isolated from Seawater, and Emended Description of the Genus Congregibacter
Hyeonsu Tak, Miri S. Park, Hyerim Cho, Yeonjung Lim, Jang-Cheon Cho
Journal of Microbiology.2024; 62(9): 739. CrossRef
Validation List no. 213. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef

Construction of a genetic linkage map and QTL mapping of agronomic traits in Auricularia auricula-judae: Li-Xin Lu , Fang-Jie Yao , Peng Wang , Ming Fang , You-Min Zhang , Wei-Tong Zhang , Xiang-Hui Kong , Jia Lu; J. Microbiol. 2017;55(10):792-799. Published online September 28, 2017; DOI: https://doi.org/10.1007/s12275-017-7241-6

50 View
0 Download
13 Crossref

Abstract

Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A. auricula-judae and amplified in A14-5, A18- 119, and the mapping population. The map consisted of 11 linkage groups (LGs) spanning 854 cM, with an average interval length of 6.57 cM. A testcross population was derived from crossing between the monokaryon A184-57 (from the wild strain A184 as a tester strain) and the mapping population. Important agronomic trait-related QTLs, including mycelium growth rate on potato dextrose agar for the mapping population, mycelium growth rate on potato dextrose agar and sawdust for the testcross population, growth period (days from inoculation to fruiting body harvesting), and yield for the testcross population, were identified using the composite interval mapping method. Six mycelium growth raterelated QTLs were identified on LG1 and LG4, two growth period-related QTLs were identified on LG2, and three yieldrelated QTLs were identified on LG2 and LG6. The results showed no linkage relationship between mycelium growth rate and growth period. The present study provides a foundation for locating genes for important agronomic characteristics in A. auricula-judae in the future.

Citations

Citations to this article as recorded by

Exploring the potential of black fungus, Auricularia auricula, as a feed additive in African catfish, Clarias gariepinus, farming
Lee Seong Wei, Alvin Amos Adrian Susin, Albaris B. Tahiluddin, Liew Vui Kien, Wendy Wee
Heliyon.2024; 10(13): e33810. CrossRef
Evaluation of Drought Tolerance and Trehalose Response in Auricularia heimuer
Jian Sun, Fangjie Yao, Lixin Lu, Youmin Zhang, Ming Fang, Xiaoxu Ma, Kaisheng Shao, Xu Sun
Horticulturae.2024; 10(12): 1312. CrossRef
Medicinal Value, Genetic Diversity, and Genetic Relationship Analysis of Auricularia cornea (Agaricomycetes) Based on ITS, ISSR, and SRAP Markers
Ping Du, Hong-Yan He, Ni-Ya Wu, Tianxu Cao, Bao-Kai Cui
International Journal of Medicinal Mushrooms.2024; 26(5): 43. CrossRef
Velvet Family Members Regulate Pigment Synthesis of the Fruiting Bodies of Auricularia cornea
Xiaoxu Ma, Lixin Lu, Youmin Zhang, Ming Fang, Kaisheng Shao, Xu Sun, Fangjie Yao, Peng Wang
Journal of Fungi.2023; 9(4): 412. CrossRef
The polysaccharides from Auricularia auricula alleviate non-alcoholic fatty liver disease via modulating gut microbiota and bile acids metabolism
Yifan Shu, Yujie Huang, Wei Dong, Xia Fan, Yi Sun, Guijie Chen, Xiaoxiong Zeng, Hong Ye
International Journal of Biological Macromolecules.2023; 246: 125662. CrossRef
Detection of quantitative trait loci underlying fruiting body and yield-related traits in Hericium erinaceus
Wenbing Gong, Xiaoya Song, Chunliang Xie, Yingjun Zhou, Zuohua Zhu, Chao Xu, Yuande Peng
Scientia Horticulturae.2022; 293: 110729. CrossRef
Analysis of the Genome Sequence of Strain GiC-126 of Gloeostereum incarnatum with Genetic Linkage Map
Wan-Zhu Jiang, Fang-Jie Yao, Ming Fang, Li-Xin Lu, You-Min Zhang, Peng Wang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao, Asif Ali Khan, Yun-Hui Wei
Mycobiology.2021; 49(4): 406. CrossRef
SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus
Youn-Lee Oh, In-Geol Choi, Kab-Yeul Jang, Min-Seek Kim, Min ji Oh, Ji-Hoon Im
Mycobiology.2021; 49(6): 589. CrossRef
Genetic linkage map construction and quantitative trait loci mapping of agronomic traits in Gloeostereum incarnatum
Wan-Zhu Jiang, Fang-Jie Yao, Li-Xin Lu, Ming Fang, Peng Wang, You-Min Zhang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao
Journal of Microbiology.2021; 59(1): 41. CrossRef
Polysaccharides from Auricularia auricula: Preparation, structural features and biological activities
Nana Chen, Hao Zhang, Xin Zong, Siyu Li, Jiaojiao Wang, Yizhen Wang, Mingliang Jin
Carbohydrate Polymers.2020; 247: 116750. CrossRef
Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map
Ming Fang, Xiaoe Wang, Ying Chen, Peng Wang, Lixin Lu, Jia Lu, Fangjie Yao, Youmin Zhang
Journal of Fungi.2020; 6(1): 37. CrossRef
A Resequencing-Based Ultradense Genetic Map of Hericium erinaceus for Anchoring Genome Sequences and Identifying Genetic Loci Associated With Monokaryon Growth
Wenbing Gong, Chunliang Xie, Yingjun Zhou, Zuohua Zhu, Yahui Wang, Yuande Peng
Frontiers in Microbiology.2020;[Epub] CrossRef
Comparative transcriptome analysis reveals relationship of three major domesticated varieties of Auricularia auricula-judae
Yuhui Zhao, Liang Wang, Dongshan Zhang, Rong Li, Tianyou Cheng, Yibi Zhang, Xueju Liu, Gary Wong, Yuguo Tang, Hui Wang, Shan Gao
Scientific Reports.2019;[Epub] CrossRef

Research Support, Non-U.S. Gov't

Enhancement of Butanol Tolerance and Butanol Yield in Clostridium acetobutylicum Mutant NT642 Obtained by Nitrogen Ion Beam Implantation: Xiao-Bo Liu , Qiu-Ya Gu , Xiao-Bin Yu , Wei Luo; J. Microbiol. 2012;50(6):1024-1028. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2289-9

32 View
0 Download
29 Scopus

Abstract: As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15.4 g/L and 22.3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyperyield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum.

Production of lipocortin-1_1-185 using a recombinant of escherichia coli: Lee, Kyung Il , Oh, Kyung Hee , Lee, Jung Hyun , Na, Do Sun , Lee, Kye Joon; J. Microbiol. 1997;35(2):123-126.

40 View
0 Download

Abstract: The aim of the present study was to optimize culture condition for the expression of lipocortin 1_1-185 in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1_1-185 gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28℃ for seed culture and 40℃ for main culture and the optimum pH was 7.0. The production of Lipocortin-1_1-185 was closely associated with cell growth and related to plasmid amplification.

Growth Properties of the Iron-reducing Bacteria, Shewanella putrefaciens IR-1 and MR-1 Coupling to Reduction of Fe(III) to Fe(II): Doo Hyun Park , Byung Hong Kim; J. Microbiol. 2001;39(4):273-278.

35 View
0 Download

Abstract: Shewanella putrefaciens IR-1 and MR-1 were cultivated by using various combinations of electron donor-acceptor, lactate-Fe(III), lactate-nitrate, pyruvate-Fe(III), pyruvate-nitrate, H_2 -acetate-Fe(III) and H_2 -acetate-nitrate. Both strains grew fermentatively on pyruvate but not on lactate without an electron acceptor. In culture with Fe(III), both strains grew on pyruvate and lactate but not on H_2 -acetate-CO_2 . In cultivation with nitrate, both strains grew on pyruvate, lactate and on H_2 -acetate-CO_2 . The growth yields of IR-1 on pyruvate, pyruvate-Fe(III) and lactate-Fe(III) were about 3.4, 3.5, and 3.6 (g cell/M substrate), respectively, but the yields on lactate-nitrate, pyruvate-nitrate and H_2 -acetate-CO_2 ?trate were about 6.8, 5.9, and 9.4 (g cell/M substrate), respectively. From the growth properties of both strains on media with Fe(III) as an electron acceptor, the bacterial growth was confirmed not to be increased by addition of Fe(III) as an electron acceptor to the growth medium, which indicates a possibility that the dissimilatory reduction of Fe(III) to Fe(II) may not be coupled to free energy production.

First
Prev
Page of 1
Next
Last

Search