Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae

Daiwen Xiao; Yongchang Yang; Hua Liu; Hua Yu; Yingjun Yan; Wenfang Huang; Wei Jiang; Weijin Liao; Qi Hu; Bo Huang

doi:10.1007/s12275-009-0092-z

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Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae: Daiwen Xiao ¹, Yongchang Yang ¹, Hua Liu ¹, Hua Yu ¹, Yingjun Yan ¹, Wenfang Huang ¹, Wei Jiang ¹, Weijin Liao ¹, Qi Hu ², Bo Huang ²; Journal of Microbiology 2009;47(5):646-650
DOI: https://doi.org/10.1007/s12275-009-0092-z
Published online: October 24, 2009

¹Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu 610072, P. R. China, ²Laboratory Medicine Department, Chongqing Medical University, Chongqing 400016, P. R. China¹Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu 610072, P. R. China, ²Laboratory Medicine Department, Chongqing Medical University, Chongqing 400016, P. R. China

Corresponding author: Wenfang Huang , Tel: 86-28-8739-4056,

Received: 24 March 2009 • Accepted: 14 June 2009

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Abstract

A method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF MS) was developed for the rapid identification of Klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. A total of 40 K. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by SELDI-TOF MS. An identification model for K. pneumoniae was established by artificial neural networks (ANNs) with classification accuracy of 100%. The model was blindly tested with 43 K. pneumoniae and 53 control bacteria again. The results showed that the model was successful with accuracy of 96.9%, sensitivity of 100% and specificity of 94.3%. This strategy is potential for rapid identification of K. pneumoniae.

Keywords: identification; K. pneumoniae; SELDI-TOF MS

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Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae

J. Microbiol. 2009;47(5):646-650. Published online October 24, 2009

DOI: https://doi.org/10.1007/s12275-009-0092-z

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