Abstract

A taxonomic study was carried out on Gsoil 142T, a bacterial strain isolated from the soil collected in a ginseng field in Pocheon province, South Korea. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this bacterium to the Gammaproteobacteria, and it was most closely related to Hydrocarboniphaga effusa ATCC BAA 332T (94.4%, 16S rRNA gene sequence similarity), Nevskia ramosa DSM 11499T (94.1%) and Alkanibacter difficilis MN154.3T (92.0%). Strain Gsoil 142T was a Gram-negative, strictly aerobic, motile, and rod-shaped bacterium. The G+C content of the genomic DNA was 69.9% and predominant ubiquinone was Q-8. Major fatty acids were summed feature 8 (C18:1 w7c and/or w6c, 36.3%), summed feature 3 (iso-C15:0 2-OH and/or C16:1 w7c, 20.6%) and C16:0 (17.4%). The major polar lipids detected in strain Gsoil 142T were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unknown glycolipid. On the basis of polyphasic evidence, it is proposed that strain Gsoil 142T should be placed in a novel genus and species, for which the name Panacagrimonas perspica gen. nov., sp. nov. is proposed. The type strain is Gsoil 142T (=KCTC 12982T =LMG 23239T).

• Keywords: 16S rRNA gene; polyphasic taxonomy; Panacagrimonas perspica gen. nov.; sp. nov.