Sequence Analysis of the Gene Encoding H Antigen in Escherichia coli Isolated from Food in Morocco

Samira Badri; Aziz Fassouane; Ingrid Filliol; Mohammed Hassar; Nozha Cohen

doi:10.1007/s12275-010-9182-1

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Sequence Analysis of the Gene Encoding H Antigen in Escherichia coli Isolated from Food in Morocco: Samira Badri ¹, Aziz Fassouane ², Ingrid Filliol ³, Mohammed Hassar ¹, Nozha Cohen ¹; Journal of Microbiology 2010;48(2):184-187
DOI: https://doi.org/10.1007/s12275-010-9182-1
Published online: May 1, 2010

¹Laboratoire de Microbiologie et d'Hygiène des Aliments et de l'Environnement, Institut Pasteur du Maroc, Casablanca 20100, Morocco, ²Laboratoire de biochimie, Département de Biologie, Faculté des Sciences d’Eljadida, Université Chouaib, Doukkali 24000, Morocco, ³Centre national de référence des Escherichia coli et Shigella, Unité de Biodiversité des Bactéries pathogènes émergentes, Institut Pasteur de Paris, Paris 75015, France¹Laboratoire de Microbiologie et d'Hygiène des Aliments et de l'Environnement, Institut Pasteur du Maroc, Casablanca 20100, Morocco, ²Laboratoire de biochimie, Département de Biologie, Faculté des Sciences d’Eljadida, Université Chouaib, Doukkali 24000, Morocco, ³Centre national de référence des Escherichia coli et Shigella, Unité de Biodiversité des Bactéries pathogènes émergentes, Institut Pasteur de Paris, Paris 75015, France

Corresponding author: Samira Badri , Tel: +212-68-30-67-18,

Received: 8 June 2009 • Accepted: 14 October 2009

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Abstract

In order to develop other molecular method useful for typing of motile and non motile Escherichia coli strains, a total of 207 strains of E. coli (133 reference strains, 74 food strains) were characterized by analysis of sequences of their amplified flagellin-encoding (fliC) gene products. The collection of reference strains was used for database building of fliC gene sequences. Application of this identification system to 74 E. coli food isolates revealed a reproducible and clear cut classification with very good correlation to results obtained by HhaI restriction of the amplified flagellin gene. The proposed determination of fliC sequences variations should be helpful for epidemiological studies.

Keywords: E. coli; H-antigen; fliC gene; sequence analysis; PCR-RFLP

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Sequence Analysis of the Gene Encoding H Antigen in Escherichia coli Isolated from Food in Morocco

J. Microbiol. 2010;48(2):184-187. Published online May 1, 2010

DOI: https://doi.org/10.1007/s12275-010-9182-1

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