Application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Monitor Effect of Biocontrol Agents on Rhizosphere Microbial Community of Hot Pepper (Capsicum annuum L.)

Young Tae Kim; Myoungho Cho; Je Yong Jeong; Hyang Burm Lee; Seung Bum Kim

doi:10.1007/s12275-010-0126-6

Articles

Page Path: HOME > J. Microbiol > Volume 48(5); 2010 > Article

Research Support, Non-U.S. Gov't
Application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Monitor Effect of Biocontrol Agents on Rhizosphere Microbial Community of Hot Pepper (Capsicum annuum L.): Young Tae Kim ¹, Myoungho Cho ¹, Je Yong Jeong ², Hyang Burm Lee ², Seung Bum Kim ¹; Journal of Microbiology 2010;48(5):566-572.
DOI: https://doi.org/10.1007/s12275-010-0126-6
Published online: November 3, 2010

¹Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea, ²Environmental Microbiology Lab, Division of Applied Bioscience and Biotechnology, Chonnam National University, Gwangju 500-757, Republic of Korea

Corresponding author: Seung Bum Kim , Tel: +82-42-821-6412,

Received: 2 April 2010 • Accepted: 14 June 2010

prev next

311 Views
0 Download
8 Crossref
11 Scopus

Full Article

Download PDF

Abstract

Microbial communities in hot pepper (Capsicum annuum L.) cultivation fields under different cultivation methods were investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis. Rhizosphere soil and leaf samples were collected from control, conventional and nature-friendly cultivation fields between May and July, 2009. Two Bacillus subtilis strains were applied to nature-friendly cultivation fields as biocontrol agents during the sampling period. Relative abundances of bacteria and plant pathogenic fungi related T-RFs were also measured to monitor the effect of biocontrol agents on potential plant pathogenic fungi. In the principal component analysis (PCA) based on T-RFLP profiles, the microbial communities from rhizosphere soil samples in July, including bacteria and fungi, showed distinct difference between nature-friendly cultivation fields and other cultivation fields. However, there was no correlation between cultivation methods and leaf microbial communities at any sampling period. Changes in the abundance of bacteria related T-RF in the rhizosphere of nature-friendly cultivation fields were observed clearly two months after application of biocontrol agent, while the abundance of plant pathogenic fungi related T-RFs significantly decreased.

Keywords: terminal restriction fragment length polymorphism;nature-friendly cultivation;B. subtilis;biocontrol

Figure & Data

References

Citations

Citations to this article as recorded by

Fusarium Fungi Pathogens, Identification, Adverse Effects, Disease Management, and Global Food Security: A Review of the Latest Research
Theodora Ijeoma Ekwomadu, Mulunda Mwanza
Agriculture.2023; 13(9): 1810. CrossRef
Impact of biocontrol microbes on soil microbial diversity in ginger (Zingiber officinaleRoscoe)
Zhiqiang Huang, Bowen Liu, Yong Yin, Fang Liang, Deshan Xie, Tiantian Han, Yongzeng Liu, Bin Yan, Qian Li, Yong Huang, Qing Liu
Pest Management Science.2021; 77(12): 5537. CrossRef
Biocontrol of strawberry fruit infected by Botrytis cinerea: Effects on the microbial communities on fruit assessed by next‐generation sequencing
Andre Freire Cruz, Geleta Dugassa Barka, Justine Sylla, Annette Reineke
Journal of Phytopathology.2018; 166(6): 403. CrossRef
Effects of the biocontrol agent Bacillus amyloliquefaciens SN16‐1 on the rhizosphere bacterial community and growth of tomato
Tingting Wan, Huihui Zhao, Wei Wang
Journal of Phytopathology.2018; 166(5): 324. CrossRef
Effect of biocontrol agent Bacillus amyloliquefaciens SN16-1 and plant pathogen Fusarium oxysporum on tomato rhizosphere bacterial community composition
Tingting Wan, Huihui Zhao, Wei Wang
Biological Control.2017; 112: 1. CrossRef
Determination of fungal community diversity in fresh and traditional Chinese fermented pepper by pyrosequencing
Lingyan Zhao, Yong Li, Liwen Jiang, Fangming Deng, Wolfgang Kneifel
FEMS Microbiology Letters.2016; 363(24): fnw273. CrossRef
Leaf Microbiota of Strawberries as Affected by Biological Control Agents
Justine Sylla, Beatrix W. Alsanius, Erika Krüger, Annette Reineke, Stephan Strohmeier, Walter Wohanka
Phytopathology®.2013; 103(10): 1001. CrossRef
Introduction of Aureobasidium pullulans to the Phyllosphere of Organically Grown Strawberries with Focus on Its Establishment and Interactions with the Resident Microbiome
Justine Sylla, Beatrix Alsanius, Erika Krüger, Annette Reineke, Monika Bischoff-Schaefer, Walter Wohanka
Agronomy.2013; 3(4): 704. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Monitor Effect of Biocontrol Agents on Rhizosphere Microbial Community of Hot Pepper (Capsicum annuum L.)

J. Microbiol. 2010;48(5):566-572. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-0126-6

Related articles