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Research Support, Non-U.S. Gov't
- Molecular Cloning and Expression Analysis of a Delta 6-Fatty Acid Desaturase Gene from Rhizopus stolonifer Strain YF6 Which Can Accumulate High Levels of Gamma-Linolenic Acid
- Xia Wan 1,2, Yinbo Zhang 1, Ping Wang 1, Mulan Jiang 1,2
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Journal of Microbiology 2011;49(1):151-154
DOI: https://doi.org/10.1007/s12275-011-0254-7
Published online: March 3, 2011
1Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China, 2Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Wuhan 430062, P. R. China1Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China, 2Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Wuhan 430062, P. R. China
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Corresponding author:
Mulan Jiang , Tel: +86-27-8683-8791,
Received: 8 July 2010 • Accepted: 5 November 2010
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Abstract
The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Δ6,9,12) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22.4% of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.
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