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- Restriction and Transcription Maps of Mitochondrial DNA of Trimorphomyces papilionaceus
- Jeoung, Won Jin , Hong, Soon Gyu , Kang, Young Won , Jung, Hack Sung
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Journal of Microbiology 1995;33(2):149-153
Department of Microbiology, College of Natural Sciences; Research Center for Molecular Microbiology, Seoul National UniversityDepartment of Microbiology, College of Natural Sciences; Research Center for Molecular Microbiology, Seoul National University
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Corresponding author:
Jung, Hack Sung ,
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Abstract
Mitochondrial DNA has been isolated from Trimorphomyces papilionaceus. By analyzing DNA fragments digested by restriction enzymes, a restriction site map has been constructured. The mtDNA of T. papilionaceus amounts to 48.5 kb in size and is circular in structure. Entire mitochondrial DNA was cloned in E coli plasmids and Northern blot hybridization was done using cloned and subcloned DNAs as probes. Based on hybridization results of mitochondrial RNA transcripts, a transcription map was prepared.
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