A Novel Ribonuclease with Potent HIV-1 Reverse Transcriptase Inhibitory Activity from Cultured Mushroom Schizophyllum commune

Yong-Chang Zhao; Guo-Qing Zhang; Tzi-Bun Ng; He-Xiang Wang

doi:10.1007/s12275-011-1098-x

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A Novel Ribonuclease with Potent HIV-1 Reverse Transcriptase Inhibitory Activity from Cultured Mushroom Schizophyllum commune: Yong-Chang Zhao ¹, Guo-Qing Zhang ^2,3, Tzi-Bun Ng ⁴, He-Xiang Wang ³; Journal of Microbiology 2011;49(5):803-808
DOI: https://doi.org/10.1007/s12275-011-1098-x
Published online: November 9, 2011

¹Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, P. R. China, ²Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, P. R. China, ³State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, P. R. China, ⁴Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China¹Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, P. R. China, ²Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, P. R. China, ³State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, P. R. China, ⁴Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China

Corresponding author: He-Xiang Wang , Tel: +86-10-627-32578,

Received: 2 March 2011 • Accepted: 25 April 2011

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Abstract

A 20-kDa ribonuclease (RNase) was purified from fresh fruiting bodies of cultured Schizophyllum commune mushrooms. The RNase was not adsorbed on Affi-gel blue gel but adsorbed on DEAE-cellulose and CM-cellulose. It exhibited maximal RNase activity at pH 6.0 and 70°C. It demonstrated the highest ribonucleolytic activity toward poly (U) (379.5 μ/mg), the second highest activity toward poly (C) (244.7 μ/mg), less activity toward poly (A) (167.4 μ/mg), and much weaker activity toward poly (G) (114.5 μ/mg). The RNase inhibited HIV-1 reverse transcriptase with an IC50 of 65 μM. No effect on [3H-methyl]-thymidine uptake by lymphoma MBL2 cells and leukemia L1210 cells was observed at 100 μM concentration of the RNase. A comparison of RNases from S. commune and Volvariella volvacea revealed that they demonstrated some similarities in N-terminal amino acid sequence, optimum pH and polyhomoribonucleotide specificity. However, some differences in chromatographic behavior and molecular mass were observed.

Keywords: Schizophyllum commune; mushroom; purification; ribonuclease

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A Novel Ribonuclease with Potent HIV-1 Reverse Transcriptase Inhibitory Activity from Cultured Mushroom Schizophyllum commune

J. Microbiol. 2011;49(5):803-808. Published online November 9, 2011

DOI: https://doi.org/10.1007/s12275-011-1098-x

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