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HOME > J. Microbiol > Volume 50(2); 2012 > Article
Research Support, Non-U.S. Gov't
Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Wheat Roots by Wheat Germ Agglutinin Labeled with Fluorescein Isothiocyanate
Jian Zhang 1, Jingyang Liu 1, Liyuan Meng 1, Zhongyou Ma 2, Xinyun Tang 1, Yuanyuan Cao 1, Leni Sun 1
Journal of Microbiology 2012;50(2):191-198.
DOI: https://doi.org/10.1007/s12275-012-1472-3
Published online: April 27, 2012
1School of Life Science, Anhui Agricultural University, Hefei 230036 Anhui Province, P. R. China, 2Department of Biology, Anhui Science and Technology University, Bengbu 233100 Anhui Province, P. R. China1School of Life Science, Anhui Agricultural University, Hefei 230036 Anhui Province, P. R. China, 2Department of Biology, Anhui Science and Technology University, Bengbu 233100 Anhui Province, P. R. China
Corresponding author:  Xinyun Tang , Tel: +86-551-5785981, 
Received: 22 September 2011   • Accepted: 7 December 2011
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Abstract

Thirty-two isolates were obtained from wheat rhizosphere by wheat germ agglutinin (WGA) labeled with fluorescein isothiocyanate (FITC). Most isolates were able to produce indole acetic acid (65.6%) and siderophores (59.3%), as well as exhibited phosphate solubilization (96.8%). Fourteen isolates displayed three plant growth-promoting traits. Among these strains, two phosphate-dissolving ones, WS29 and WS31, were evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum Wan33). Strain WS29 and WS31 significantly promoted the development of lateral roots by 34.9% and 27.6%, as well as increased the root dry weight by 25.0% and 25.6%, respectively, compared to those of the control. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, both isolates were determined to belong to the genus Bacillus. The proportion of isolates showing the properties of plant growth-promoting rhizobacteria (PGPR) was higher than in previous reports. The efficiency of the isolation of PGPR strains was also greatly increased by WGA labeled with FITC. The present study indicated that WGA could be used as an effective tool for isolating PGPR strains with high affinity to host plants from wheat roots. The proposed approach could facilitate research on biofertilizers or biocontrol agents.

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    Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Wheat Roots by Wheat Germ Agglutinin Labeled with Fluorescein Isothiocyanate
    J. Microbiol. 2012;50(2):191-198.   Published online April 27, 2012
    DOI: https://doi.org/10.1007/s12275-012-1472-3
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