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Production of lipocortin-1_1-185 using a recombinant of escherichia coli
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HOME > J. Microbiol > Volume 35(2); 1997 > Article
Production of lipocortin-1_1-185 using a recombinant of escherichia coli
Lee, Kyung Il , Oh, Kyung Hee , Lee, Jung Hyun , Na, Do Sun 1, Lee, Kye Joon
Journal of Microbiology 1997;35(2):123-126

Department of Microbiology, College of Natural Sciences, Seoul National University; ¹Medical School, Ul San UniversityDepartment of Microbiology, College of Natural Sciences, Seoul National University; ¹Medical School, Ul San University
Corresponding author:  Lee, Kye Joon ,
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Abstract

The aim of the present study was to optimize culture condition for the expression of lipocortin 1_1-185 in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1_1-185 gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28℃ for seed culture and 40℃ for main culture and the optimum pH was 7.0. The production of Lipocortin-1_1-185 was closely associated with cell growth and related to plasmid amplification.

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    Production of lipocortin-1_1-185 using a recombinant of escherichia coli
    J. Microbiol. 1997;35(2):123-126.
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