NOTE] The Activity of Phosphoinositide-Specific Phospholipase C Is Required for Vegetative Growth and Cell Wall Regeneration in Coprinopsis cinerea

Young Taek Oh; Chun-Seob Ahn; Kyung-Jin Lee; Jeong-Geun Kim; Hyeon-Su Ro; Jae Won Kim; Chang-Won Lee

doi:10.1007/s12275-012-2004-x

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NOTE] The Activity of Phosphoinositide-Specific Phospholipase C Is Required for Vegetative Growth and Cell Wall Regeneration in Coprinopsis cinerea: Young Taek Oh , Chun-Seob Ahn , Kyung-Jin Lee , Jeong-Geun Kim , Hyeon-Su Ro , Jae Won Kim , Chang-Won Lee; Journal of Microbiology 2012;50(4):689-692.
DOI: https://doi.org/10.1007/s12275-012-2004-x
Published online: August 25, 2012

Department of Microbiology, College of Natural Science/ Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Microbiology, College of Natural Science/ Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea

Corresponding author: Jae Won Kim , Tel: +82-55-772-1322/+82-55-772-1325,
Chang-Won Lee , Tel: +82-55-772-1322/+82-55-772-1325,

Received: 3 January 2012 • Accepted: 7 May 2012

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Abstract

Three isotypes of phosphoinositide-specific phospholipase C designated CcPLC1, CcPLC2, and CcPLC3 were identified in Coprinopsis cinerea, through a search of the genome sequence database. The functional role of the PI-PLCs were studied by using U73122, which specifically inhibits the activity of PI-PLC. The specificity of the inhibitor effect was confirmed by using an inactive structural analog U73433. The inhibition of PI-PLCs activity resulted in severely retarded germination of basidiospores and oidia, reduced hyphal growth, knobbly hyphal tips with many irregular side branches, and aberrant (branch-like structure) clamp cells. Furthermore, U73122 definitely inhibited cell wall formation. Here we report that PI-PLCs play important roles in various aspects of C. cinerea biology.

Keywords: phosphoinositide-specific phospholipase C (PI-PLC);Coprinopsis cinerea;U73122

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NOTE] The Activity of Phosphoinositide-Specific Phospholipase C Is Required for Vegetative Growth and Cell Wall Regeneration in Coprinopsis cinerea

J. Microbiol. 2012;50(4):689-692. Published online August 25, 2012

DOI: https://doi.org/10.1007/s12275-012-2004-x

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