A New Quorum-Sensing Inhibitor Attenuates Virulence and Decreases Antibiotic Resistance in Pseudomonas aeruginosa

Yu-Xiang Yang; Zhen-Hua Xu; Yu-Qian Zhang; Jing Tian; Li-Xing Weng; Lian-Hui Wang

doi:10.1007/s12275-012-2149-7

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Research Support, Non-U.S. Gov't
A New Quorum-Sensing Inhibitor Attenuates Virulence and Decreases Antibiotic Resistance in Pseudomonas aeruginosa: Yu-Xiang Yang ¹, Zhen-Hua Xu ¹, Yu-Qian Zhang ¹, Jing Tian ², Li-Xing Weng ¹, Lian-Hui Wang ²; Journal of Microbiology 2012;50(6):987-993
DOI: https://doi.org/10.1007/s12275-012-2149-7
Published online: December 30, 2012

¹Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P. R. China, ²Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu 210046, P. R. China¹Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P. R. China, ²Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu 210046, P. R. China

Corresponding author: Lian-Hui Wang , Tel: +86-21-65642817,

Received: 19 March 2012 • Accepted: 19 July 2012

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Abstract

Quorum sensing (QS) has been a novel target for the treatment of infectious diseases. Here structural analogs of Pseudomonas aeruginosa autoinducer N-acyl homoserine lactone (AHL) were investigated for QS inhibitor (QSI) activity and a novel QSI was discovered, N-decanoyl-L-homoserine benzyl ester (C2). Virulence assays showed that C2 downregulated total protease and elastase activities, as well as the production of rhamnolipid, that are controlled by QS in P. aeruginosa wild-type strain PAO1 without affecting growth. C2 was also shown to inhibit swarming motility of PAO1. Using a microdilution checkerboard method, we identified synergistic interactions between C2 and several antibiotics, tobramycin, gentamycin, cefepime, and meropenem. Data from real-time RT-PCR suggested that C2 inhibited the expression of lasR (29.67%), lasI (21.57%), rhlR (28.20%), and rhlI (29.03%).

Keywords: Pseudomonas aeruginosa; quorum sensing; inhibitor; virulence factor; swarming; antimicrobial susceptibility

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A New Quorum-Sensing Inhibitor Attenuates Virulence and Decreases Antibiotic Resistance in Pseudomonas aeruginosa

J. Microbiol. 2012;50(6):987-993. Published online December 30, 2012

DOI: https://doi.org/10.1007/s12275-012-2149-7

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