Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-09.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Quantification of Toxigenic Microcystis spp. in Freshwaters by Quantitative Real-time PCR Based on the Microcystin Synthetase A Gene
Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 51(1); 2013 > Article
Research Support, Non-U.S. Gov't
Quantification of Toxigenic Microcystis spp. in Freshwaters by Quantitative Real-time PCR Based on the Microcystin Synthetase A Gene
Kyoung-Hee Oh 1, Dong-Hwan Jeong 2, Young-Cheol Cho 1
Journal of Microbiology 2013;51(1):18-24
DOI: https://doi.org/10.1007/s12275-013-2354-z
Published online: March 2, 2013
1Department of Environmental Engineering, Chungbuk National University, Cheongju 361-763, Republic of Korea, 2National Institute of Environmental Research, Incheon 404-708, Republic of Korea1Department of Environmental Engineering, Chungbuk National University, Cheongju 361-763, Republic of Korea, 2National Institute of Environmental Research, Incheon 404-708, Republic of Korea
Corresponding author:  Young-Cheol Cho , Tel: +82-43-261-3577, 
Received: 10 July 2012   • Accepted: 16 November 2012
prev next
  • 3 Views
  • 0 Download
  • 0 Crossref
  • 14 Scopus
Full Article

Abstract

A method to estimate the abundance of toxigenic Microcystis in environmental samples by using quantitative real-time PCR was developed and optimized. The basis of this method is the amplification of a highly conserved region of the mcyA gene within the microcystin synthetase gene cluster. Using this method, the average copy number of mcyA gene per cell in toxigenic Microcystis strains was estimated. The molecular markers and method developed in this study can be used to monitor toxigenic strains of Microcystis in Korean freshwaters, in which harmful cyanobacterial blooms are routinely found.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Quantification of Toxigenic Microcystis spp. in Freshwaters by Quantitative Real-time PCR Based on the Microcystin Synthetase A Gene
    J. Microbiol. 2013;51(1):18-24.   Published online March 2, 2013
    DOI: https://doi.org/10.1007/s12275-013-2354-z
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP