Abstract

A Gram-positive, facultatively aerobic, rod-shaped, nonmotile, terminal spore-forming bacterium, designated strain G20T, was isolated from soil collected in a mountain region of Suwon, South Korea. On the basis of 16S rRNA gene sequence similarity, this strain was shown to be related to Rummeliibacillus pycnus NBRC 101231T (97.4%) and Rummeliibacillus stabekisii KSC-SF6gT (95.7%). DNA-DNA hybridization studies showed 42% and 50% similarity of strain G20T with R. pycnus NBRC 101231Tand R. stabekisii KSCSF6gT, respectively. The DNA G+C content of G20T was 37.8 mol%, the major cellular fatty acids were iso-C15:0 and anteiso-C15:0, and the predominant menaquinones were MK-7 and MK-8. On the basis of phylogenetic, chemotaxonomic, and phenotypic characteristics, we propose this strain to be a novel species and the third member of genus Rummeliibacillus. We suggest the name Rummeliibacillus suwonensis sp. nov. The type strain is G20T (KACC 17316T =KEMB 9005-003T =JCM 19065T).

• Keywords: Rummeliibacillus suwonensis;soil;spore-forming